O-GLYCOSIDASE
- Product Name
- O-GLYCOSIDASE
- CAS No.
- 9032-92-2
- Chemical Name
- O-GLYCOSIDASE
- Synonyms
- O-Glycanase;EC 3.2.1.97;O-GLYCOSIDASE;O-GLYCAN PEPTIDE HYDROLASE;endo-α-n-acetylgalactosaminidase;O-GLYCOSIDASE, EXPRESSED IN E. COLI;ENDO-ALPHA-N-ACETYLGALACTOSAMINIDASE;o-glycosidase from streptococcus pneumoniae;Endo-α-N-acetylgalactosaminidase, O-Glycanase;O-Glycosidase from Streptococcus pneumoniae, Recombinant
- CBNumber
- CB4337902
- Formula Weight
- 0
- MOL File
- Mol file
O-GLYCOSIDASE Property
- storage temp.
- 2-8°C
- form
- buffered aqueous solution
Safety
- WGK Germany
- 3
N-Bromosuccinimide Price
- Product number
- G1163
- Product name
- O-Glycosidase from Streptococcus pneumoniae
- Purity
- recombinant, expressed in E. coli, buffered aqueous solution
- Packaging
- 0.04unit
- Price
- $625
- Updated
- 2024/03/01
O-GLYCOSIDASE Chemical Properties,Usage,Production
Uses
O-Glycosidase is used to release the Gal-β(1,3)-GaINAc unit from O-glycans (glycoproteins or glycopeptides). Bindings to serine as well as to threonine are hydrolyzed. Substituents at the disaccharide (e.g., sialic acid) prevent the hydrolysis and, therefore, have to be removed chemically or enzymatically (e.g., with neuraminidase from Arthrobacter or from Vibrio cholerae).
O-Glycosidase has been used to selectively deglycosylated chondroitin sulfate proteoglycans (CSPGs).
General Description
O-Glycan-peptide hydrolase
O-Glycopeptide endo-D-galactosyl-N-acetyl-α-galactosamino hydrolase
O-Glycosidase is isolated from the culture filtrate of Diplococcus pneumoniae
Biochem/physiol Actions
Releases unsubstituted Ser- and Thr-linked β-Gal-(1→3)-α-GalNAc (Core 1 type O-glycan) from glycoproteins. Substitutions of the disaccharide core with sialic acid, lactosamine (galactose-N-acetyl glucosamine), or fucose will block hydrolysis and prevent the liberation of the oligosaccharide from the protein. Pretreament with glycolytic enzymes to remove substituent saccharides from the O-glycan may be needed prior to cleavage using O-glycosidase..
O-GLYCOSIDASE Preparation Products And Raw materials
Raw materials
Preparation Products
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