ChemicalBook > CAS DataBase List > Riboflavin

Riboflavin

Product Name
Riboflavin
CAS No.
83-88-5
Chemical Name
Riboflavin
Synonyms
VITAMIN B2;RIBOFLAVINE;(-)-RIBOFLAVIN;Flavaxin;Riboflavin Vitamin B2;Riboflavin Universal;Vitamin B2 (Riboflavine);VIT B2;LACTOFLAVIN;Riboflavin CRS
CBNumber
CB4383318
Molecular Formula
C17H20N4O6
Formula Weight
376.36
MOL File
83-88-5.mol
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Riboflavin Property

Melting point:
290 °C (dec.)(lit.)
alpha 
-135 º (c=5, 0.05 M NaOH)
Boiling point:
504.93°C (rough estimate)
Density 
1.2112 (rough estimate)
refractive index 
-135 ° (C=0.5, JP Method)
Flash point:
9℃
storage temp. 
2-8°C
solubility 
Very slightly soluble in water, practically insoluble in ethanol (96 per cent). Solutions deteriorate on exposure to light, especially in the presence of alkali. It shows polymorphism (5.9).
form 
Powder
pka
1.7(at 25℃)
color 
Yellow to orange
PH
5.5-7.2 (0.07g/l, H2O, 20°C)
Odor
Slight odour
PH Range
6
Water Solubility 
0.07 g/L (20 ºC)
Sensitive 
Light Sensitive
Merck 
14,8200
BRN 
97825
BCS Class
1
Stability:
Stable, but light-sensitive. Incompatible with strong oxidizing agents, reducing agents, bases, calcium, metallic salts. May be moisture sensitive.
InChIKey
AUNGANRZJHBGPY-SCRDCRAPSA-N
LogP
-2.009 (est)
CAS DataBase Reference
83-88-5(CAS DataBase Reference)
NIST Chemistry Reference
Riboflavine(83-88-5)
EPA Substance Registry System
Riboflavin (83-88-5)
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Safety

Hazard Codes 
F,T
Risk Statements 
11-23/24/25-39/23/24/25
Safety Statements 
24/25-45-36/37-16
RIDADR 
UN1230 - class 3 - PG 2 - Methanol, solution
WGK Germany 
1
RTECS 
VJ1400000
F 
8-10-21
TSCA 
Yes
HS Code 
29362300
Hazardous Substances Data
83-88-5(Hazardous Substances Data)
Toxicity
LD50 in rats (g/kg): >10 orally, 5.0 s.c., 0.56 i.p. (Unna, Greslin)
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H226Flammable liquid and vapour

H370Causes damage to organs

Precautionary statements

P210Keep away from heat/sparks/open flames/hot surfaces. — No smoking.

P280Wear protective gloves/protective clothing/eye protection/face protection.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
V-067
Product name
(−)-Riboflavin
Purity
100?μg/mL (1% ammonium acetate in 50:50 methanol:water), certified reference material, ampule of 1?mL, Cerilliant?
Packaging
1mL
Price
$129
Updated
2024/03/01
Sigma-Aldrich
Product number
PHR1054
Product name
Riboflavin
Purity
Pharmaceutical Secondary Standard; Certified Reference Material
Packaging
1g
Price
$99.3
Updated
2024/03/01
Sigma-Aldrich
Product number
555682
Product name
Riboflavin-CAS83-88-5-Calbiochem
Purity
Awatersolublevitamininvolvedinthesynthesisofflavinmononucleotide(FMN)
Packaging
10g
Price
$48
Updated
2024/03/01
Sigma-Aldrich
Product number
5.00257
Product name
Riboflavin
Purity
E 101 (i)
Packaging
2.5kg
Price
$1364
Updated
2024/03/01
Sigma-Aldrich
Product number
47861
Product name
Riboflavin (B2)
Purity
analytical standard
Packaging
1000mg
Price
$23.2
Updated
2024/03/01
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Riboflavin Chemical Properties,Usage,Production

Description

A water-soluble B fraction was found in the 1920s to contain a yellow, fluorescent growth factor called riboflavin in England and vitamin G in the United States. In the early 1930s, several groups found the coenzyme forms of riboflavin 50-phosphate (flavin mononucleotide) and the further conjugate with adenylic acid (flavin adenine dinucleotide).

Chemical Properties

VITAMIN B2 (Riboflavin). Some earlier designations for this substance included vitamin G, lactoflavin, hepatoflavin, ovoflavin, verdoflavin. The chemical name is 6,7-dimethyl-9-d-l’ribityl isolloxazine. Riboflavin is a complex pigment with a green fluorescence.

Chemical Properties

Yellow to orange/yellow crystalline powd

Physical properties

Riboflavin is moderately soluble in water (10–13 mg/dl) and ethanol but insoluble in ether, chloroform, and acetone. It is soluble but unstable under alkaline conditions.
The catalytic functions of riboflavin are carried out primarily at positions N-1, N-5, and C-4 of the isoalloxazine nucleus. In addition, the methyl group at C-8 participates in covalent bonding with enzyme proteins. The flavin coenzymes are highly versatile redox cofactors because they can participate in either one- or two electron redox reactions
Riboflavin antagonists include analogs of the isoalloxazine ring (e.g., diethylri boflavin, dichlororiboflavin) and the ribityl side chain (e.g., d-araboflavin, d-galactoflavin, 7-ethylriboflavin).

Originator

Hyflavin ,Endo,US,1948

Occurrence

(-)-Riboflavin is a nutritional factor found in milk, eggs, malted barley, liver, kidney, heart, leafy vegetables. Richest natural source is yeast. Minute amounts present in all plant and animal cells. Vitamin (enzyme cofactor).

Uses

Vitamin B2 (riboflavin) is produced by yeast from glucose, urea, and mineral salts in an aerobic fermentation.

Uses

Nutritional factor found in milk, eggs, malted barley, liver, kidney, heart, leafy vegetables. Richest natural source is yeast. Minute amounts present in all plant and animal cells. Vitamin (enzyme cofactor).

Uses

Vitamin B2; Vitamin cofactor; LD50(rat) 560 mg/kg ip

Uses

riboflavin (Vitamin B2) is used in skin care preparations as an emollient. It can be found in sun care products as a suntan enhancer. Medicinally, it is used for the treatment of skin lesions.

Uses

Riboflavin is the water-soluble vitamin b2 required for healthy skin and the building and maintaining of body tissues. it is a yellow to orange-yellow crystalline powder. it acts as a coenzyme and carrier of hydrogen. it is stable to heat but may dissolve and be lost in cooking water. it is relatively stable to storage. sources include leafy vegetables, cheese, eggs, and milk.

Definition

ChEBI: D-Ribitol in which the hydroxy group at position 5 is substituted by a 7,8-dimethyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl moiety. It is a nutritional factor found in milk, eggs, malted barley, liver, kidney, heart, and leafy vege ables, but the richest natural source is yeast. The free form occurs only in the retina of the eye, in whey, and in urine; its principal forms in tissues and cells are as flavin mononucleotide and flavin-adenine dinucleotide.

Manufacturing Process

100 g of riboflavin and 3 of potassium carbonate are suspended in 500 cc of the aqueous formaldehyde solution and the mixture is stirred at 30°C for 8 hours. At the end of this period, 5 cc of glacial acetic acid and 1 liter of methanol are added, with stirring. The solution is freed from undissolved material by filtration and the clear solution is poured slowly at about 20°C to 22°C with vigorous stirring into 8 liters of anhydrous acetone. The resultant precipitate is filtered off, washed repeatedly with anhydrous acetone and with ether, and then dried at room temperature and with vacuum. The resultant dried powder is dissolved in hot water at 95°C to give an aqueous solution of 20% by weight. This solution is kept in the dark at room temperature for 3 to 4 weeks, after which time a large amount of material crystallizes out of the solution. This crystallized material is removed by filtration and recrystallized from hot water. A small amount of dark red insoluble material is filtered from the hot solution. This recrystallization step is repeated four times. The resultant end product is monomethylol riboflavin, which crystallized in small orange clusters. It has a melting point of 232°C to 234°C with decomposition, and it becomes dark when heated above 225°C.

brand name

Flavaxin (Sterling Winthrop).

Therapeutic Function

Enzyme cofactor vitamin source

General Description

The conflicting results were eventually found to be due,in part, to deficiencies in study animals not just of vitamin B2,but also vitamin B3 (niacin), the cause of human forms of pellagra,and/or vitamin B6 (pyridoxine), another cause of dermatitis.Likewise, treatments with vitamin B2 were inconsistentbecause the early sources of this vitamin contained otherB vitamins. Vitamin B2 was eventually isolated from eggwhites in 1933 and produced synthetically in 1935. Thename riboflavine was officially accepted in 1960; althoughthe term was in common use before then. In 1966, IUPACchanged it to riboflavin, which is in common use today.Riboflavin is synthesized by all green plants and by mostbacteria and fungi. Therefore, riboflavin is found, at least insmall amounts, in most foods. Foods that are naturally highin riboflavin include milk and other dairy products, meat,eggs, fatty fish, and dark green vegetables.
Chemically, riboflavin is an N-glycoside of flavin, alsoknown as lumichrome, and the sugar, ribitol .Flavin is derived from the Latin word flavus for “yellow”because of the yellow color of its crystals and yellow fluorescenceunder UV light. Riboflavin is heat stable but easilydegraded by light. Its systematic names are 7,8-dimethyl-10-ribitylisoalloxazine and 7,8-dimethyl-10-(D-ribo-2,3,4,5-tetrahydroxypentyl)isoalloxazine.

Biochem/physiol Actions

Riboflavin serves as a precursor for the active enzyme cofactors riboflavin 5′-monophosphate (also called flavin mononucleotide or FMN) and flavin adenine dinucleotide (FAD). Riboflavin deficiency in the diet results in a well-defined syndrome known as ariboflavinosis, Riboflavin exhibits protective effects against tumor development and cardiovascular disease. Its deficiency often affects metabolism involving redox reactions. Riboflavin is found essential for iron absorption, gastrointestinal development, neurogenesis, corneal vascularization and corneal opacity.

Clinical Use

Severe riboflavin deficiency is known as ariboflavinosis, andtreatment or prevention of this condition is the only provenuse of riboflavin. Ariboflavinosis is most commonly associatedwith multiple vitamin deficiency as a result of alcoholismin developed countries. Because of the large numberof enzymes requiring riboflavin as a coenzyme, deficienciescan lead to a wide range of abnormalities. In adults seborrheicdermatitis, photophobia, peripheral neuropathy, anemia, andoropharyngeal changes including angular stomatitis, glossitis,and cheilosis, are often the first signs of riboflavin deficiency.In children, cessation of growth can also occur. As the deficiencyprogresses, more severe pathologies develop untildeath ensues. Riboflavin deficiency may also produce teratogeniceffects and alter iron handling leading to anemia.

Safety Profile

Poison by intravenous route. Moderately toxic by intraperitoneal and subcutaneous routes. Mutation data reported. When heated to decomposition it emits toxic fumes of NOx.

Environmental Fate

Physicochemical Properties
Riboflavin has the appearance of a yellow to orange amorphous solid and imparts an orange color to the B vitamin tablets. Riboflavin has a melting point of 290°C, a density of 1.65, and a refractive index of 135°. The pKa is 9.888 and log P is 0.095. Riboflavin has solubility in water of 0.1 g l-1.
Exposure Routes and Pathways
The route of exposure is oral. Dietary sources of riboflavin and its coenzymes include broccoli, spinach, asparagus, enriched flour, yeast, eggs, milk, cheese, mackerel, trout, poultry, liver, and kidneys.
Toxicokinetics
Riboflavin, which is only moderately water soluble, is absorbed from the gastrointestinal tract but is limited to about 27 mg at any one time from an oral dose given to an adult. Hence, mega doses would not be expected to increase significantly the total amount absorbed. It is hepatically metabolized, protein bound, and widely distributed to tissue; however, little is stored in the liver, spleen, heart, and kidneys. Riboflavin is excreted renally as metabolites, which have been oxidatively cleaved in the ribityl side chain and converted to hydroxymethyls in the ring methyl functions. Riboflavin in excess of daily body needs is excreted unchanged in the urine. Riboflavin exhibits biphasic pharmacokinetics with initial and terminal half-lives of 1.4 and 14 h, respectively.

Properties and Applications

yellow

Purification Methods

It crystallises from H2O as a yellow-orange powder in three different forms with differing amounts of H2O. It melts if placed in an oil bath at 250o, but decomposes at 280o if heated at a rate of 5o/minute. It is also purified by crystallisation from 2M acetic acid, then extracted with CHCl3 to remove lumichrome impurity. [Smith & Metzler J Am Chem Soc 85 3285 1963.] Its solubility in H2O is 1g in 3-15L depending on the crystal structure. Its solubility in EtOH at 25o is 4.5mg in 100mL. Store it in the dark because it is decomposed to lumichrome by UV light. [Pearson The Vitamins vol V pp1-96 1967 and vol VII pp 1-96 1972, Gy.gy and Pearson eds, Academic Press, Beilstein 26 IV 2542.]

Riboflavin Preparation Products And Raw materials

Raw materials

Barbituric acid Sodium dithionite Alloxan Bone glue Starch 3,4-Dimethylaniline corn syrup

Preparation Products

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View Lastest Price from Riboflavin manufacturers

HangZhou RunYan Pharma Technology Co.,LTD.
Product
Riboflavin, Vitamin B2 83-88-5
Price
US $0.00-0.00/g
Min. Order
10g
Purity
99% HPLC
Supply Ability
10000
Release date
2024-09-20
Hubei Lidu New Material Technology Co., Ltd
Product
RIBOFLAVIN 83-88-5
Price
US $0.00/kg
Min. Order
1kg
Purity
0.99
Supply Ability
20tons
Release date
2024-10-30
Hebei Zhuanglai Chemical Trading Co.,Ltd
Product
Vitamin B2 Riboflavin 83-88-5
Price
US $180.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
20ton
Release date
2024-05-31

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