SP6 RNA POLYMERASE
- Product Name
- SP6 RNA POLYMERASE
- Chemical Name
- SP6 RNA POLYMERASE
- Synonyms
- SP6 RNA;SP6 RNA POLYMERASE
- CBNumber
- CB4778598
- Formula Weight
- 0
- MOL File
- Mol file
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SP6 RNA POLYMERASE Property
- storage temp.
- -20°C
- form
- solution
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N-Bromosuccinimide Price
Sigma-Aldrich
- Product number
- RPOLSP6-RO
- Product name
- SP6 RNA Polymerase
- Purity
- from Escherichia coli BL 21/pSR3
- Packaging
- 1000units
- Price
- $187
- Updated
- 2024/03/01
Sigma-Aldrich
- Product number
- RPOLSP6-RO
- Product name
- SP6 RNA Polymerase
- Purity
- from Escherichia coli BL 21/pSR3
- Packaging
- 5000units
- Price
- $715
- Updated
- 2024/03/01
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SP6 RNA POLYMERASE Chemical Properties,Usage,Production
Uses
SP6 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from an SP6 promoter. The synthesis can be performed with labeled NTPs to generate highly labeled RNA. Synthesized RNA can be used in many applications, including:
- RNA or DNA blotting techniques
- In situ hybridization
- RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing.
- Synthesis of capped RNA in vitro with addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA can be introduced into cells to suppress the expression of the corresponding genes.
- The synthesis of antisense RNA probes
General Description
With this system, homogeneously labeled single-stranded RNA can be generated. Transcripts can be non-radioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [α-32P] or [α-35S] labeled nucleotides.
Contents:
- SP6 RNA Polymerase, ≥20 U/μl, in buffer, pH 7.9
- Transcription Buffer, 10x concentrated
SP6 RNA POLYMERASE Preparation Products And Raw materials
Raw materials
Preparation Products
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SP6 RNA POLYMERASE Suppliers
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- 021-61415566 800-8193336
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- China
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- 021-34675735 19147740836
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SP6 RNA POLYMERASERelated Search:
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