ChemicalBook > CAS DataBase List > BCECF-AM

BCECF-AM

Product Name
BCECF-AM
CAS No.
117464-70-7
Chemical Name
BCECF-AM
Synonyms
BCECF-AM;BCECF--1M;BCECF--1M 1MG;Bcecf-AmSolution;bcecf-acetoxymethyl;BCECF-Acetoxymethyl ester;BCECF/AM - CAS 117464-70-7 - Calbiochem;BCECF.AM ESTER, 1 MG/ML IN*ANHYDROUS DMSO;2,7-BIS(2-CARBOXYETHYL)-5,6-CARBOXYFLUORESCEIN;2',7'-bis(2-carboxyethyl)-5(6)-*carboxyfluorescei
CBNumber
CB5111514
Molecular Formula
C42H40O21
Formula Weight
880.75
MOL File
117464-70-7.mol
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BCECF-AM Property

Boiling point:
692.41°C (rough estimate)
Density 
1.2837 (rough estimate)
refractive index 
1.6500 (estimate)
Flash point:
85 °C
storage temp. 
-20°C
form 
White to off-white solid
color 
Orange to red
CAS DataBase Reference
117464-70-7(CAS DataBase Reference)
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
22-26-36
WGK Germany 
3
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P321Specific treatment (see … on this label).

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P362Take off contaminated clothing and wash before reuse.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
216254
Product name
BCECF/AM
Packaging
1mg
Price
$193
Updated
2024/03/01
Cayman Chemical
Product number
15922
Product name
BCECF-Acetoxymethyl ester
Purity
≥95%
Packaging
1mg
Price
$117
Updated
2024/03/01
Cayman Chemical
Product number
15922
Product name
BCECF-Acetoxymethyl ester
Purity
≥95%
Packaging
5mg
Price
$521
Updated
2024/03/01
Cayman Chemical
Product number
15922
Product name
BCECF-Acetoxymethyl ester
Purity
≥95%
Packaging
25mg
Price
$1540
Updated
2021/12/16
TRC
Product number
B119183
Product name
BCECF/AM
Packaging
1mg
Price
$220
Updated
2021/12/16
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BCECF-AM Chemical Properties,Usage,Production

Description

BCECF AM ester is a widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at 490 nm vs. the emission intensity when excited at 440 nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF AM can also be used to investigate intracellular changes in other ions, including potassium.

Chemical Properties

pale yellow to yellow powder

Uses

Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.

General Description

Material is an isomeric mixture of lactones, acetates, and AM esters

Biological Activity

Non-charged, non-fluorescent pH indicator. Readily and rapidly diffuses through cell membranes.

BCECF-AM Preparation Products And Raw materials

Raw materials

Preparation Products

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BCECF-AM Suppliers

3B Pharmachem (Wuhan) International Co.,Ltd.
Tel
821-50328103-801 18930552037
Fax
86-21-50328109
Email
3bsc@sina.com
Country
China
ProdList
15839
Advantage
69
Beijing Ouhe Technology Co., Ltd
Tel
010-82967028 13552068683
Fax
+86-10-82967029
Email
2355560935@qq.com
Country
China
ProdList
12390
Advantage
60
Dalian Meilun Biotech Co., Ltd.
Tel
0411-62910999 13889544652
Email
meilunui@163.com
Country
China
ProdList
4727
Advantage
58
ShangHai YuanYe Biotechnology Co., Ltd.
Tel
021-61312847 13636370518
Fax
021-55068248
Email
shyysw007@163.com
Country
China
ProdList
4940
Advantage
60
Artis Chemistry (Shanghai) Co. Ltd.
Tel
86-21-60936353
Fax
86-21-60936352
Country
China
ProdList
335
Advantage
58
Thermo Fisher Scientific
Tel
800-810-5118
Fax
+86-10-84193589
Email
cnchemical@thermofisher.com
Country
China
ProdList
17770
Advantage
75
Shanghai Aladdin Bio-Chem Technology Co.,LTD
Tel
400-400-6206333 18521732826
Fax
021-50323701
Email
market@aladdin-e.com
Country
China
ProdList
25003
Advantage
65
Chengdu HuaXia Chemical Reagent Co. Ltd
Tel
400-1166-196 13458535857
Fax
QQ:800101999
Email
cdhxsj@163.com
Country
China
ProdList
13350
Advantage
58
Finetech Industry Limited
Tel
027-87465837 19945049750
Fax
027-8777-2287
Email
sales@finetechnology-ind.com
Country
China
ProdList
9648
Advantage
58
Sigma-Aldrich
Tel
021-61415566 800-8193336
Email
orderCN@merckgroup.com
Country
China
ProdList
51456
Advantage
80

117464-70-7, BCECF-AMRelated Search:


  • 2',7'-BIS-(2-CARBOXYETHYL)-5-(AND-6)-CARBOXYFLUORESCEIN, ACETOXYMETHYL ESTER
  • 2,7-BIS(2-CARBOXYETHYL)-5,6-CARBOXYFLUORESCEIN
  • 2',7'-BIS(2-CARBOXYETHYL)-5(6)-CARBOXYFLUORESCEIN ACETOXYMETHYL ESTER
  • 2',7'-BIS-(CARBOXYETHYL)-5(6')-CARBOXYFLUORESCEIN ACETOXYMETHYL ESTER
  • 2',7'-bis(2-carboxyethyl)-5(6)-*carboxyfluorescei
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  • Bcecf-AmSolution
  • 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester mixed isomers
  • bcecf-acetoxymethyl
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  • BCECF-AM solution, Spiro(isobenzofuran-1(3H),9μ-(9H)xanthene)-2μ,7μ-dipropanoic acid
  • 2',7'-Bis(2-acetoxymethoxycarbonylethyl)-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid acetoxymethyl ester
  • 2,7-Bis[2-(acetoxymethoxycarbonyl)ethyl]-3,6-dihydroxy-3'-oxospiro[9H-xanthene-9,1'(3'H)-isobenzofuran]-6'-carboxylic acid acetoxymethyl ester
  • 2',7'-Biscarboxyethyl-6-carboxyfluorescein acetoxymethyl ester
  • BCECF-AM
  • 3',6'-bis(Acetyloxy)-5(or6)-[[(acetyloxy)methoxy]carbonyl]-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoicacid2',7'-bis[(acetyloxy)methyl]ester
  • BCECF/AM - CAS 117464-70-7 - Calbiochem
  • BCECF-Acetoxymethyl ester
  • BCECF--1M
  • BCECF--1M 1MG
  • SPIRO(ISOBENZOFURAN-1(3H),9'-(9H)XANTHENE)-2',7'-DIPROPANOIC ACID
  • 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxyMet
  • 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethy
  • 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester
  • 117464-70-7
  • C42H40O21
  • C39H36O19
  • Cell Signaling and Neuroscience
  • BioChemical
  • Cell Biology
  • Ion Probes
  • Ion Channels
  • Monovalent Ion Channels
  • Voltage-gated Ion Channels