XBA I

Product Name
XBA I
Chemical Name
XBA I
Synonyms
XBA I
CBNumber
CB5137507
Molecular Formula
C97H123N38O58P9
Formula Weight
3028
MOL File
Mol file
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XBA I Property

storage temp. 
-20°C
form 
solution
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
XBAI-RO
Product name
Xba I
Purity
from Xanthomonas campestris
Packaging
1000units
Price
$43.3
Updated
2024/03/01
Sigma-Aldrich
Product number
XBAI-RO
Product name
Xba I
Purity
from Xanthomonas campestris
Packaging
5000units
Price
$183
Updated
2024/03/01
Sigma-Aldrich
Product number
XBAI-RO
Product name
Xba I
Purity
from Xanthomonas campestris
Packaging
20000units
Price
$539
Updated
2022/05/15
Usbiological
Product number
X0989
Product name
Xba I
Packaging
3000U
Price
$286
Updated
2021/12/16
Biorbyt Ltd
Product number
orb94057
Product name
Xba I
Packaging
17500units
Price
$302.6
Updated
2021/12/16
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XBA I Chemical Properties,Usage,Production

Uses

The restriction enzyme Xba I has been used for the digestion of genomic DNA.

General Description

Xba I recognizes the sequence T↓*CTAGA and generates fragments with 5′-cohesive termini. The enzyme needs at least two nucleotides around the target sequence before it will cut the cleavage site.

Compatible ends
Xba I ends are compatible with fragments generated by Avr I, Nhe I, and Spe I.

Isoschizomers
The enzyme is not known to have isoschizomers.

Methylation sensitivity
Xba I digestion of DNA is inhibited by the dam gene product of E. coli, which methylates the 6N position of adenine in the sequence GATC. The enzyme is also inhibited by 5-methylcytosine or 5-hydroxymethylcytosine at the site (*) indicated on the recognition sequence.

Activity in SuRE/Cut Buffer System
Buffer printed in bold face type is the buffer recommended for optimal activity:
A B L M H
100% 75-100% 75-100% 75-100% 100%

Relative activity in complete PCR mix
Relative activity in PCR mix (Taq DNA Polymerase buffer) is 60%. The PCR mix contained ?DNA, primers, 10 mM Tris-HCl (pH 8.3, +20°C), 50 mM KCl, 1.5 mM MgCl2, 200 ?M dNTPs, 2.5 U Taq DNA polymerase. The mix was subjected to 25 amplification cycles. Activity in reaction buffer of Pwo SuperYield DNA Polymerase PCR Mix is 25%. When supplemented with GC-RICH Solution, activity increases to 100%.

Incubation temperature
+37°C


Number of cleavage sites on different DNAs
λ Ad2 SV40 ?X174 M13mp7 M13mp18 pBR322 pBR328 pUC18
1 5 0 0 0 1 0 0 1

PFGE tested
Xba I has been tested in Pulsed-Field Gel Electrophoresis (on bacterial chromosomes). For cleavage of genomic DNA (E. coli C 600) embedded in agarose for PFGE analysis, we recommend 10 U enzyme per ?g DNA and approximately 4 hour incubation time.

Ligation and recutting assay
Xba I fragments obtained by complete digestion of 1 ?g pUC18 DNA are ligated with 1 U T4 DNA Ligase in a volume of 10 ?l by incubation for 16 hours at +4°C in 66 mM Tris-HCl, 5 mM MgCl2, 5 mM Dithiothreitol, 1 mM ATP, pH 7.5 (at +20°C) resulting in >90% recovery of pUC18 DNA.
Subsequent re-cutting with Xba I yields >95% of the typical pattern of pUC18 × Xba I fragments.

XBA I Preparation Products And Raw materials

Raw materials

Preparation Products

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XBA I Suppliers

FERMENTAS Inc.
Tel
--
Fax
--
Country
United States
ProdList
335
Advantage
82
Amersham Biosciences, Inc.
Tel
--
Fax
--
Country
United States
ProdList
1270
Advantage
85
Nacalai Tesque, Inc.
Tel
--
Fax
--
Email
info-tech@nacalai.co.jp
Country
Japan
ProdList
6046
Advantage
75
Roche Applied Science
Tel
--
Fax
--
Email
biochemts.us@roche.com
Country
United States
ProdList
696
Advantage
85
Qbiogene
Tel
--
Fax
--
Email
orders@qbiogene.com
Country
United States
ProdList
925
Advantage
69
MP Biomedicals, Inc.
Tel
--
Fax
--
Country
United States
ProdList
6561
Advantage
81