SPH I
- Product Name
- SPH I
- CAS No.
- 85270-15-1
- Chemical Name
- SPH I
- Synonyms
- APA I;SPH I;Einecs 286-569-8;APA I RESTRICTION ENZYME;SPH I RESTRICTION ENZYME;restriction endonuclease Sph I;RESTRICTION ENDONUCLEASE APA I;RESTRICTION ENDONUCLEASES APA I;RESTRICTION ENDONUCLEASES SPH I;apa i from acetobacter pasteurianus
- CBNumber
- CB0280961
- Formula Weight
- 0
- MOL File
- Mol file
SPH I Property
- storage temp.
- −20°C
- form
- buffered aqueous glycerol solution
Safety
- WGK Germany
- 1
N-Bromosuccinimide Price
- Product number
- A2298-63
- Product name
- Apa I
- Packaging
- 5000U
- Price
- $327
- Updated
- 2021/12/16
- Product number
- orb93990
- Product name
- Apa I
- Packaging
- 25000units
- Price
- $486.2
- Updated
- 2021/12/16
- Product number
- orb94025
- Product name
- Sph I
- Packaging
- 250units
- Price
- $74.8
- Updated
- 2021/12/16
- Product number
- orb93990
- Product name
- Apa I
- Packaging
- 5000units
- Price
- $151.3
- Updated
- 2021/12/16
- Product number
- orb94025
- Product name
- Sph I
- Packaging
- 1250Units
- Price
- $302.6
- Updated
- 2021/12/16
SPH I Chemical Properties,Usage,Production
General Description
Compatible ends
Apa I ends are not compatible with those generated by any other known restriction enzymes.
Isoschizomers
Apa I is an isoschizomer to Bsp 120 I and Psp OMI.
Methylation sensitivity
Apa I is inhibited by 5′-methylcytosine at the sites indicated (*) in the recognition sequence.
Activity in SuRE/Cut Buffer System
Buffer printed in bold face type is the buffer recommended for optimal activity:
ABLMH
100%10-25%50-75%50-75%0-10%
Relative activity in complete PCR mix
Relative activity in PCR mix (Taq DNA Polymerase buffer) is 100%. The PCR mix contained target DNA, primers, 10 mM Tris-HCl (pH 8.3, +20°C), 50 mM KCl, 1.5 mM MgCl2, 200 μM dNTPs, 2.5 U Taq DNA polymerase. The mix was subjected to 25 amplification cycles. Activity in reaction buffer of Pwo SuperYield DNA Polymerase PCR Mix is 10%. When supplemented with GC-RICH Solution activity is increased to >100%.
Incubation temperature
+30°C
Unit definition
One unit is the enzyme activity that completely cleaves 1 μg ? × Hind III fragments in 1 hour at +30°C in a total volume of 25 μl SuRE/Cut Buffer A.
Heat inactivation
The enzyme can be heat inactivated by incubating it for 15 minutes at +65°C.
Number of cleavage sites on different DNAs
λAd2SV40φ X174M13mp7M13mp18pBR322pBR328pUC18
112 1000000
Ligation and recutting assay
Apa I fragments obtained by complete digestion of 1 μg λ × Hind III fragments are ligated with 1 U T4 DNA Ligase in a volume of 10 μl by incubation for 16 hours at +4°C in 66 mM Tris-HCl, 5 mM MgCl2, 5 mM Dithiothreitol, 1 mM ATP, pH 7.5 (at +20°C) resulting in >95% recovery of 1 μg λ × Hind III fragments.
Subsequent re-cutting with Apa I yields >95% of the typical pattern of λ × Hind III× Apa I fragments.
SPH I Preparation Products And Raw materials
Raw materials
Preparation Products
SPH I Suppliers
- Tel
- 821-50328103-801 18930552037
- Fax
- 86-21-50328109
- 3bsc@sina.com
- Country
- China
- ProdList
- 15848
- Advantage
- 69
- Tel
- 021-61415566 800-8193336
- orderCN@merckgroup.com
- Country
- China
- ProdList
- 51471
- Advantage
- 80
- Tel
- +86-0371-86658258 +8613203830695
- Fax
- 0371-86658258
- laboratory@coreychem.com
- Country
- China
- ProdList
- 30250
- Advantage
- 58
- Tel
- 021-61998208 18217752821
- Fax
- 021-37680378
- sales@klbio.cn
- Country
- China
- ProdList
- 9618
- Advantage
- 58
- Tel
- --
- Fax
- --
- sales@advtechind.com
- Country
- United States
- ProdList
- 6106
- Advantage
- 58
- Tel
- 1-516-855-7709
- Fax
- 1-631-938-8127
- info@creative-enzymes.com
- Country
- United States
- ProdList
- 8748
- Advantage
- 58