ChemicalBook > CAS DataBase List > SPH I

SPH I

Product Name
SPH I
CAS No.
85270-15-1
Chemical Name
SPH I
Synonyms
APA I;SPH I;Einecs 286-569-8;APA I RESTRICTION ENZYME;SPH I RESTRICTION ENZYME;restriction endonuclease Sph I;RESTRICTION ENDONUCLEASE APA I;RESTRICTION ENDONUCLEASES APA I;RESTRICTION ENDONUCLEASES SPH I;apa i from acetobacter pasteurianus
CBNumber
CB0280961
Formula Weight
0
MOL File
Mol file
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SPH I Property

storage temp. 
−20°C
form 
buffered aqueous glycerol solution
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Safety

WGK Germany 
1
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Usbiological
Product number
A2298-63
Product name
Apa I
Packaging
5000U
Price
$327
Updated
2021/12/16
Biorbyt Ltd
Product number
orb93990
Product name
Apa I
Packaging
25000units
Price
$486.2
Updated
2021/12/16
Biorbyt Ltd
Product number
orb94025
Product name
Sph I
Packaging
250units
Price
$74.8
Updated
2021/12/16
Biorbyt Ltd
Product number
orb93990
Product name
Apa I
Packaging
5000units
Price
$151.3
Updated
2021/12/16
Biorbyt Ltd
Product number
orb94025
Product name
Sph I
Packaging
1250Units
Price
$302.6
Updated
2021/12/16
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SPH I Chemical Properties,Usage,Production

General Description

Compatible ends
Apa I ends are not compatible with those generated by any other known restriction enzymes.

Isoschizomers
Apa I is an isoschizomer to Bsp 120 I and Psp OMI.

Methylation sensitivity
Apa I is inhibited by 5′-methylcytosine at the sites indicated (*) in the recognition sequence.

Activity in SuRE/Cut Buffer System
Buffer printed in bold face type is the buffer recommended for optimal activity:
ABLMH
100%10-25%50-75%50-75%0-10%

Relative activity in complete PCR mix
Relative activity in PCR mix (Taq DNA Polymerase buffer) is 100%. The PCR mix contained target DNA, primers, 10 mM Tris-HCl (pH 8.3, +20°C), 50 mM KCl, 1.5 mM MgCl2, 200 μM dNTPs, 2.5 U Taq DNA polymerase. The mix was subjected to 25 amplification cycles. Activity in reaction buffer of Pwo SuperYield DNA Polymerase PCR Mix is 10%. When supplemented with GC-RICH Solution activity is increased to >100%.

Incubation temperature
+30°C

Unit definition
One unit is the enzyme activity that completely cleaves 1 μg ? × Hind III fragments in 1 hour at +30°C in a total volume of 25 μl SuRE/Cut Buffer A.

Heat inactivation
The enzyme can be heat inactivated by incubating it for 15 minutes at +65°C.

Number of cleavage sites on different DNAs
λAd2SV40φ X174M13mp7M13mp18pBR322pBR328pUC18
112 1000000

Ligation and recutting assay
Apa I fragments obtained by complete digestion of 1 μg λ × Hind III fragments are ligated with 1 U T4 DNA Ligase in a volume of 10 μl by incubation for 16 hours at +4°C in 66 mM Tris-HCl, 5 mM MgCl2, 5 mM Dithiothreitol, 1 mM ATP, pH 7.5 (at +20°C) resulting in >95% recovery of 1 μg λ × Hind III fragments.
Subsequent re-cutting with Apa I yields >95% of the typical pattern of λ × Hind III× Apa I fragments.

SPH I Preparation Products And Raw materials

Raw materials

Preparation Products

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SPH I Suppliers

China 46 Germany 2 Japan 1 Switzerland 1 United Kingdom 1 United States 12 Global 63
3B Pharmachem (Wuhan) International Co.,Ltd.
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85270-15-1, SPH IRelated Search:

METHYL ISOCYANOACETATE Cupric acetylacetonate Ethyl isocyanoacetate TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) Aluminum acetylacetonate Ferric acetylacetonate 2,4-PENTANEDIONE, SILVER DERIVATIVE DICHLORO(ETHYLENEDIAMINE)PLATINUM(II) SALCOMINE TERT-BUTYL ISOCYANIDE 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE N-BUTYLISOCYANIDE TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) COBALT(II) ACETYLACETONATE Benzyl isocyanide Tosylmethyl isocyanide PHENYLSELENOL Sp

  • Einecs 286-569-8
  • restriction endonuclease apa I from*acetobacter P
  • Nuclease, restriction endodeoxyribo-, SphI
  • RESTRICTION ENDONUCLEASE APA I FROM*ACET OBACTER PAS
  • RESTRICTION ENDONUCLEASE SPH I FROM*STRE PTOMYCES
  • apa i from acetobacter pasteurianus
  • sph i from streptomyces phaeochromogenes
  • RESTRICTION ENDONUCLEASE APA I
  • restriction endonuclease Sph I
  • APA I RESTRICTION ENZYME
  • APA I
  • SPH I
  • SPH I RESTRICTION ENZYME
  • RESTRICTION ENDONUCLEASES APA I
  • RESTRICTION ENDONUCLEASES SPH I
  • 85270-15-1
  • Restriction Endonucleases
  • Molecular Biology Enzymes
  • Molecular Biology
  • BioChemical
  • 3.1.x.x Acting on esters
  • 3.x.x.x Hydrolases
  • Molecular Biology
  • Molecular Biology Enzymes
  • Molecular Biology Tested
  • Restriction EndonucleasesApplication Index
  • Restriction EnzymesEnzyme Class Index