DPN I

Product Name: DPN I
CAS No.: 81295-14-9
Chemical Name: DPN I
Synonyms: DPN I;DPN I RESTRICTION ENZYME;restriction endonuclease Dpn I;RESTRICTION ENDONUCLEASES DPN I;dpn i from diplococcus pneumoniae;restriction endonuclease dpn I from*diplococcus P;RESTRICTION ENDONUCLEASE DPN I FROM*DIPL OCOCCUS PNE
CBNumber: CB0256555
Formula Weight: 0
MOL File: Mol file

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DPN I Property

storage temp.: -20°C
form: buffered aqueous glycerol solution

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Safety

RIDADR: UN3373 - class 6.2 Biological substance, Category B
WGK Germany: 3

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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: DPNI-RO
Product name: Dpn I
Purity: from Diplococcus pneumoniae
Packaging: 1000units
Price: $357
Updated: 2024/03/01

American Custom Chemicals Corporation

Product number: ENZ0008473
Product name: Dpn I
Purity: 95.00%
Packaging: 5MG
Price: $451.58
Updated: 2021/12/16

Biorbyt Ltd

Product number: orb93993
Product name: Dpn I
Packaging: 200units
Price: $74.8
Updated: 2021/12/16

Biorbyt Ltd

Product number: orb93993
Product name: Dpn I
Packaging: 1000units
Price: $302.6
Updated: 2021/12/16

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DPN I Chemical Properties,Usage,Production

Uses

In PCR-mediated mutagenesis, DpnI nuclease has been used for the digestion of wild type DNA after PCR.

General Description

Isoschizomers
The enzyme is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II and Sau3A I.
Methylation sensitivity
The enzyme is only inhibited by the occurrence of 5-methylcytosine at the indicated site (*) if no 6-methyladenine is present.
Typical ligation and recutting assay
Dpn I fragments obtained by complete digestion of 1μg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in 10μl buffer that contains 66mM Tris-HCl, 5mM MgCl₂, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Dpn I (yielding the typical pattern of pBR322 × Dpn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.

DPN I Preparation Products And Raw materials

Raw materials

Preparation Products

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DPN I Suppliers

China 51 Germany 1 Switzerland 1 United States 7 Global 60

3B Pharmachem (Wuhan) International Co.,Ltd.

Tel: 821-50328103-801 18930552037
Fax: 86-21-50328109
Email: 3bsc@sina.com
Country: China
ProdList: 15839
Advantage: 69

Sigma-Aldrich

Tel: 021-61415566 800-8193336
Email: orderCN@merckgroup.com
Country: China
ProdList: 51456
Advantage: 80

Career Henan Chemica Co

Tel: +86-0371-86658258 +8613203830695
Fax: 0371-86658258
Email: laboratory@coreychem.com
Country: China
ProdList: 30240
Advantage: 58

Shanghai Kanglang Biotechnology Co., Ltd.

Tel: 021-61998208 18217752821
Fax: 021-37680378
Email: sales@klbio.cn
Country: China
ProdList: 9618
Advantage: 58

Wuhan Chemstan Biotechnology Co., Ltd.

Tel: 027-65317797 15926423062
Fax: QQ：422450190
Email: 422450190@qq.com
Country: China
ProdList: 10359
Advantage: 58

Shanghai Yuchun Biotechnology Co., Ltd

Tel: 021-021-52273538 17321241593
Fax: 13564515386
Email: 1503472510@qq.com
Country: China
ProdList: 5261
Advantage: 58

81295-14-9, DPN IRelated Search:

TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) Benzyl isocyanide Tosylmethyl isocyanide N-BUTYLISOCYANIDE COBALT(II) ACETYLACETONATE 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE PHENYLSELENOL Tris(2,4-pentanedionato)chroMiuM(III) Cupric acetylacetonate TERT-BUTYL ISOCYANIDE METHYL ISOCYANOACETATE 2,4-PENTANEDIONE, SILVER DERIVATIVE Aluminum acetylacetonate TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) SALCOMINE Ethyl isocyanoacetate Ferric acetylacetonate α-Nicotinamide adenine dinucleotide

RESTRICTION ENDONUCLEASES DPN I

DPN I

DPN I RESTRICTION ENZYME

restriction endonuclease dpn I from*diplococcus P

RESTRICTION ENDONUCLEASE DPN I FROM*DIPL OCOCCUS PNE

dpn i from diplococcus pneumoniae

restriction endonuclease Dpn I

81295-14-9

Restriction Endonucleases

Molecular Biology Enzymes

Molecular Biology

BioChemical

Restriction EnzymesEnzyme Class Index

3.1.x.x Acting on esters

3.x.x.x Hydrolases

Molecular Biology

Molecular Biology Enzymes

Molecular Biology Tested

Restriction EndonucleasesApplication Index