ChemicalBook > CAS DataBase List > DPN I

DPN I

Product Name
DPN I
CAS No.
81295-14-9
Chemical Name
DPN I
Synonyms
DPN I;DPN I RESTRICTION ENZYME;restriction endonuclease Dpn I;RESTRICTION ENDONUCLEASES DPN I;dpn i from diplococcus pneumoniae;restriction endonuclease dpn I from*diplococcus P;RESTRICTION ENDONUCLEASE DPN I FROM*DIPL OCOCCUS PNE
CBNumber
CB0256555
Formula Weight
0
MOL File
Mol file
More
Less

DPN I Property

storage temp. 
-20°C
form 
buffered aqueous glycerol solution
More
Less

Safety

RIDADR 
UN3373 - class 6.2 Biological substance, Category B
WGK Germany 
3
More
Less

Hazard and Precautionary Statements (GHS)

More
Less

N-Bromosuccinimide Price

Sigma-Aldrich
Product number
DPNI-RO
Product name
Dpn I
Purity
from Diplococcus pneumoniae
Packaging
1000units
Price
$357
Updated
2024/03/01
American Custom Chemicals Corporation
Product number
ENZ0008473
Product name
Dpn I
Purity
95.00%
Packaging
5MG
Price
$451.58
Updated
2021/12/16
Biorbyt Ltd
Product number
orb93993
Product name
Dpn I
Packaging
200units
Price
$74.8
Updated
2021/12/16
Biorbyt Ltd
Product number
orb93993
Product name
Dpn I
Packaging
1000units
Price
$302.6
Updated
2021/12/16
More
Less

DPN I Chemical Properties,Usage,Production

Uses

In PCR-mediated mutagenesis, DpnI nuclease has been used for the digestion of wild type DNA after PCR.

General Description

Isoschizomers
The enzyme is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II and Sau3A I.
Methylation sensitivity
The enzyme is only inhibited by the occurrence of 5-methylcytosine at the indicated site (*) if no 6-methyladenine is present.
Typical ligation and recutting assay
Dpn I fragments obtained by complete digestion of 1μg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in 10μl buffer that contains 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Dpn I (yielding the typical pattern of pBR322 × Dpn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.

DPN I Preparation Products And Raw materials

Raw materials

Preparation Products

More
Less

DPN I Suppliers

China 51 Germany 1 Switzerland 1 United States 7 Global 60
3B Pharmachem (Wuhan) International Co.,Ltd.
Tel
821-50328103-801 18930552037
Fax
86-21-50328109
Email
3bsc@sina.com
Country
China
ProdList
15848
Advantage
69
Sigma-Aldrich
Tel
021-61415566 800-8193336
Email
orderCN@merckgroup.com
Country
China
ProdList
51471
Advantage
80
Career Henan Chemica Co
Tel
+86-0371-86658258 +8613203830695
Fax
0371-86658258
Email
laboratory@coreychem.com
Country
China
ProdList
30250
Advantage
58
Shanghai Kanglang Biotechnology Co., Ltd.
Tel
021-61998208 18217752821
Fax
021-37680378
Email
sales@klbio.cn
Country
China
ProdList
9618
Advantage
58
Wuhan Chemstan Biotechnology Co., Ltd.
Tel
027-65317797 15926423062
Fax
QQ：422450190
Email
422450190@qq.com
Country
China
ProdList
10351
Advantage
58
Shanghai Yuchun Biotechnology Co., Ltd
Tel
021-021-52273538 17321241593
Fax
13564515386
Email
1503472510@qq.com
Country
China
ProdList
5261
Advantage
58
Shanghai Haoran Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
470003480@QQ.COM
Country
CHINA
ProdList
6968
Advantage
58
Shanghai Yaji Biotechnology Co., Ltd.
Tel
--
Fax
--
Country
CHINA
ProdList
6857
Advantage
58
Hangzhou Capsule Technology Co., Ltd.
Tel
--
Fax
--
Email
biofire@163.com
Country
CHINA
ProdList
143
Advantage
58
Beijing Dongge Boye Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
1861586486@qq.com
Country
CHINA
ProdList
6652
Advantage
58
Shanghai Qiming Biological Technology Co., Ltd.
Tel
--
Fax
--
Country
CHINA
ProdList
6503
Advantage
58
Beijing Xinhua Lvyuan Technology Co., Ltd.
Tel
--
Fax
--
Email
724124204@qq.com
Country
CHINA
ProdList
1962
Advantage
58
Shanghai Lianmai Biological Engineering Co., Ltd.
Tel
--
Fax
--
Email
shlmai@163.com
Country
CHINA
ProdList
6700
Advantage
58
Shanghai Yuqi Biological Technology Co., Ltd.
Tel
--
Fax
--
Email
847627550@qq.com
Country
CHINA
ProdList
6087
Advantage
58
BEIJING BOMED GENETIC TECHNOLOGY CO., LTD.
Tel
13366569263
Email
biomedweb@163.com
Country
CHINA
ProdList
80
Advantage
58
Shanghai Minmin Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
1914109725@qq.com
Country
CHINA
ProdList
6530
Advantage
58
NEB (Beijing)
Tel
--
Fax
--
Email
info@neb-china.com
Country
CHINA
ProdList
63
Advantage
58
Beijing Fubo Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
info@f-biology.com
Country
CHINA
ProdList
6039
Advantage
58
Shanghai Binzhi Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
htbinbio@163.com
Country
CHINA
ProdList
2327
Advantage
58
Beijing Shengke Boyuan Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
978560024@qq.com
Country
CHINA
ProdList
6011
Advantage
58
Beijing Bai Ao Si Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
yangmei@bioneeds.net
Country
CHINA
ProdList
6999
Advantage
58
Beijing Huaxia Ocean Technology Co., Ltd.
Tel
--
Fax
--
Email
huaxiabio@163.com
Country
CHINA
ProdList
6122
Advantage
58
Shanghai Kemin Biological Technology Co., Ltd.
Tel
--
Fax
--
Email
2633804423@qq.com
Country
CHINA
ProdList
3497
Advantage
58
Beijing Bi Orange Blue Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
bo49855987@163.com
Country
CHINA
ProdList
6827
Advantage
58
Shanghai Beinuo Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
beinuobio@163.com
Country
CHINA
ProdList
6580
Advantage
58
Wuhan Boot Biotechnology Co., Ltd.
Tel
--
Fax
--
Country
CHINA
ProdList
6829
Advantage
58
Beijing Baiao Innovation Technology Co., Ltd.
Tel
--
Fax
--
Email
3007606172@qq.com
Country
CHINA
ProdList
6005
Advantage
58
Beijing Bomais Technology Development Co., Ltd.
Tel
--
Fax
--
Email
15210716102@139.com
Country
CHINA
ProdList
6573
Advantage
58
Shanghai Hao Biological Technology Co., Ltd.
Tel
--
Fax
--
Email
market@xlswkj.com
Country
CHINA
ProdList
6805
Advantage
58
Shanghai Zhenyu Biological Technology Co., Ltd.
Tel
--
Fax
--
Email
2836767067@qq.com
Country
CHINA
ProdList
6970
Advantage
58
Sangon Biotech (Shanghai) Co., Ltd.
Tel
--
Fax
--
Email
market3@sangon.com
Country
CHINA
ProdList
6952
Advantage
58
Shanghai Shengzhao Biological Technology Co., Ltd.
Tel
--
Fax
--
Email
bussiness@shengzhaobio.com
Country
CHINA
ProdList
330
Advantage
58
Nanjing Sai Ruirui Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
sales@shrbio.com
Country
CHINA
ProdList
6873
Advantage
58
Shanghai Hao Biological Technology Co., Ltd.
Tel
--
Fax
--
Email
shxiyuanbio@163.com
Country
CHINA
ProdList
6921
Advantage
58
Boao Pike
Tel
--
Fax
--
Email
lyn@biopike.com.cn
Country
CHINA
ProdList
6629
Advantage
58
Shenzhen Branch Science and Technology Co., Ltd.
Tel
--
Fax
--
Email
zikerbio@163.com
Country
CHINA
ProdList
6478
Advantage
58
Beijing Dingguo Changsheng Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
xiaoyang@dlcs100.com
Country
CHINA
ProdList
2452
Advantage
58
Wuhan Aibotek Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
tech@abclonal.com
Country
CHINA
ProdList
6796
Advantage
58
Shanghai Yuda Industrial Co., Ltd.
Tel
--
Fax
--
Email
lucy@runwelltac.com
Country
CHINA
ProdList
6107
Advantage
58
Shanghai Mengze Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
luxionggang@126.com
Country
CHINA
ProdList
6393
Advantage
58
Beijing Zhijie Fangyuan Technology Co., Ltd.
Tel
--
Fax
--
Email
15810200961@163.com
Country
CHINA
ProdList
6984
Advantage
58
Suzhou Ruinuode Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
2090855807@qq.com
Country
CHINA
ProdList
6063
Advantage
58
Xiamen Huijia Biological Technology Co., Ltd.
Tel
--
Fax
--
Country
CHINA
ProdList
6979
Advantage
58
Shanghai Hengfei Biological Technology Co., Ltd.
Tel
--
Fax
--
Email
13851015143@163.com
Country
CHINA
ProdList
6146
Advantage
58
Guangzhou Weijia Technology Co., Ltd.
Tel
--
Fax
--
Email
WHIGA22@126.COM
Country
CHINA
ProdList
6748
Advantage
58
Xiamen Research Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
1562893815@qq.com
Country
CHINA
ProdList
6942
Advantage
58
Shanghai Caiyou Industrial Co., Ltd.
Tel
--
Fax
--
Email
15821073967@163.com
Country
CHINA
ProdList
6894
Advantage
58
Shanghai Haoyang Biological Technology Co., Ltd.
Tel
--
Fax
--
Email
596799915@QQ.COM
Country
CHINA
ProdList
2905
Advantage
58
Beijing Dakco is Biotechnology Co., Ltd.
Tel
--
Fax
--
Email
bj_info@dakewe.net
Country
CHINA
ProdList
6348
Advantage
58
Beyotime Biotechnology
Tel
--
Fax
--
Email
info@beyotime.com
Country
CHINA
ProdList
4658
Advantage
58

81295-14-9, DPN IRelated Search:

TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) Benzyl isocyanide Tosylmethyl isocyanide N-BUTYLISOCYANIDE COBALT(II) ACETYLACETONATE 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE PHENYLSELENOL Tris(2,4-pentanedionato)chroMiuM(III) Cupric acetylacetonate TERT-BUTYL ISOCYANIDE METHYL ISOCYANOACETATE 2,4-PENTANEDIONE, SILVER DERIVATIVE Aluminum acetylacetonate TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) SALCOMINE Ethyl isocyanoacetate Ferric acetylacetonate α-Nicotinamide adenine dinucleotide

  • RESTRICTION ENDONUCLEASES DPN I
  • DPN I
  • DPN I RESTRICTION ENZYME
  • restriction endonuclease dpn I from*diplococcus P
  • RESTRICTION ENDONUCLEASE DPN I FROM*DIPL OCOCCUS PNE
  • dpn i from diplococcus pneumoniae
  • restriction endonuclease Dpn I
  • 81295-14-9
  • Restriction Endonucleases
  • Molecular Biology Enzymes
  • Molecular Biology
  • BioChemical
  • Restriction EnzymesEnzyme Class Index
  • 3.1.x.x Acting on esters
  • 3.x.x.x Hydrolases
  • Molecular Biology
  • Molecular Biology Enzymes
  • Molecular Biology Tested
  • Restriction EndonucleasesApplication Index