ChemicalBook > CAS DataBase List > DPN I

DPN I

Product Name
DPN I
CAS No.
81295-14-9
Chemical Name
DPN I
Synonyms
DPN I;DPN I RESTRICTION ENZYME;restriction endonuclease Dpn I;RESTRICTION ENDONUCLEASES DPN I;dpn i from diplococcus pneumoniae;restriction endonuclease dpn I from*diplococcus P;RESTRICTION ENDONUCLEASE DPN I FROM*DIPL OCOCCUS PNE
CBNumber
CB0256555
Formula Weight
0
MOL File
Mol file
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DPN I Property

storage temp. 
-20°C
form 
buffered aqueous glycerol solution
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Safety

RIDADR 
UN3373 - class 6.2 Biological substance, Category B
WGK Germany 
3
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
DPNI-RO
Product name
Dpn I
Purity
from Diplococcus pneumoniae
Packaging
1000units
Price
$357
Updated
2024/03/01
American Custom Chemicals Corporation
Product number
ENZ0008473
Product name
Dpn I
Purity
95.00%
Packaging
5MG
Price
$451.58
Updated
2021/12/16
Biorbyt Ltd
Product number
orb93993
Product name
Dpn I
Packaging
200units
Price
$74.8
Updated
2021/12/16
Biorbyt Ltd
Product number
orb93993
Product name
Dpn I
Packaging
1000units
Price
$302.6
Updated
2021/12/16
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DPN I Chemical Properties,Usage,Production

Uses

In PCR-mediated mutagenesis, DpnI nuclease has been used for the digestion of wild type DNA after PCR.

General Description

Isoschizomers
The enzyme is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II and Sau3A I.
Methylation sensitivity
The enzyme is only inhibited by the occurrence of 5-methylcytosine at the indicated site (*) if no 6-methyladenine is present.
Typical ligation and recutting assay
Dpn I fragments obtained by complete digestion of 1μg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in 10μl buffer that contains 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Dpn I (yielding the typical pattern of pBR322 × Dpn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.

DPN I Preparation Products And Raw materials

Raw materials

Preparation Products

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DPN I Suppliers

China 51 Germany 1 Switzerland 1 United States 7 Global 60
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81295-14-9, DPN IRelated Search:

TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)DYSPROSIUM(III) Benzyl isocyanide Tosylmethyl isocyanide N-BUTYLISOCYANIDE COBALT(II) ACETYLACETONATE 1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE PHENYLSELENOL Tris(2,4-pentanedionato)chroMiuM(III) Cupric acetylacetonate TERT-BUTYL ISOCYANIDE METHYL ISOCYANOACETATE 2,4-PENTANEDIONE, SILVER DERIVATIVE Aluminum acetylacetonate TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III) SALCOMINE Ethyl isocyanoacetate Ferric acetylacetonate α-Nicotinamide adenine dinucleotide

  • RESTRICTION ENDONUCLEASES DPN I
  • DPN I
  • DPN I RESTRICTION ENZYME
  • restriction endonuclease dpn I from*diplococcus P
  • RESTRICTION ENDONUCLEASE DPN I FROM*DIPL OCOCCUS PNE
  • dpn i from diplococcus pneumoniae
  • restriction endonuclease Dpn I
  • 81295-14-9
  • Restriction Endonucleases
  • Molecular Biology Enzymes
  • Molecular Biology
  • BioChemical
  • Restriction EnzymesEnzyme Class Index
  • 3.1.x.x Acting on esters
  • 3.x.x.x Hydrolases
  • Molecular Biology
  • Molecular Biology Enzymes
  • Molecular Biology Tested
  • Restriction EndonucleasesApplication Index