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GMBS

Product Name
GMBS
CAS No.
80307-12-6
Chemical Name
GMBS
Synonyms
4-Maleimidobutyric acid N-hydroxysuccinimide ester;2,5-Dioxopyrrolidin-1-yl 4-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)butanoate;GMBS;GMBS Crosslinker;CIVENTICHEM CV-2656;4-Maleimidobutyric acid NHS;4-Maleimidobutyric acid-NHS ester;(GMBS):4-Maleimidobutyric acid NHS;N-(4-Maleimibutyryloxy)succinimide;N-SUCCINIMIDYL 4-MALEIMIDOBUTYRATE
CBNumber
CB5267080
Molecular Formula
C12H12N2O6
Formula Weight
280.23
MOL File
80307-12-6.mol
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GMBS Property

Melting point:
123-129 °C
Boiling point:
461.3±47.0 °C(Predicted)
Density 
1.49±0.1 g/cm3(Predicted)
storage temp. 
2-8°C
solubility 
chloroform: 100 mg/mL
form 
powder
pka
-2.27±0.20(Predicted)
color 
White to Pale Yellow
BRN 
6427689
Stability:
Hygroscopic, Moisture Sensitive
InChI
InChI=1S/C12H12N2O6/c15-8-3-4-9(16)13(8)7-1-2-12(19)20-14-10(17)5-6-11(14)18/h3-4H,1-2,5-7H2
InChIKey
PVGATNRYUYNBHO-UHFFFAOYSA-N
SMILES
N1(CCCC(ON2C(=O)CCC2=O)=O)C(=O)C=CC1=O
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-36
WGK Germany 
3
10-21
HS Code 
29280000
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P405Store locked up.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
63175
Product name
4-Maleimidobutyric acid N-hydroxysuccinimide ester
Purity
≥98.0% (HPLC)
Packaging
25mg
Price
$102
Updated
2025/07/31
Sigma-Aldrich
Product number
63175
Product name
4-Maleimidobutyric acid N-hydroxysuccinimide ester
Purity
≥98.0% (HPLC)
Packaging
100mg
Price
$370
Updated
2025/07/31
Sigma-Aldrich
Product number
442630-M
Product name
4-Maleimidobutyric acid N-hydroxysuccinimide ester
Packaging
100mg
Price
$316
Updated
2025/07/31
TCI Chemical
Product number
S0399
Product name
N-Succinimidyl 4-Maleimidobutyrate [Cross-linking Reagent]
Purity
>98.0%(HPLC)(N)
Packaging
100mg
Price
$36
Updated
2025/07/31
TCI Chemical
Product number
S0399
Product name
N-Succinimidyl 4-Maleimidobutyrate [Cross-linking Reagent]
Purity
>98.0%(HPLC)(N)
Packaging
1g
Price
$196
Updated
2025/07/31
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GMBS Chemical Properties,Usage,Production

Chemical Properties

White Solid

Uses

A short, sulfhydryl and amino reactive heterobifunctional crosslinking reagent. Introduces maleimide groups into IgM

Uses

A heterobifunctional cross-linking reagent with amine and sulfhydryl reactivity. Useful in enzyme immunoassays

reaction suitability

reagent type: linker

Synthesis

6066-82-6

116965-29-8

80307-12-6

General procedure for the synthesis of 4-maleimidobutanoic acid-N-hydroxysuccinimide ester from N-hydroxysuccinimide and 4-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)butyryl chloride: N-maleimidobutanoic acid (99 g, 0.54 mol) and acetonitrile (300 ml) were placed in a 500 ml three-necked flask and thionyl chloride was slowly added ( 93.6 g, 0.82 mol). The reaction mixture was heated to reflux during which a large amount of gas release was observed. After the reaction was refluxed for 4 h, the mixture became clarified and acetonitrile and excess thionyl chloride were subsequently removed by concentration. 200 ml of acetonitrile was added to remove the residual acid to give the crude product N-maleimidobutyryl chloride. The crude product was dissolved in 200 ml of acetonitrile and added dropwise to 300 ml of acetonitrile solution containing N-hydroxysuccinimide (69 g, 0.6 mol) and tri-n-butylamine (148 g, 0.8 mol), during which the temperature was controlled to be no more than 20 °C using an ice water bath. The titration process lasted for 2 hours and after completion the reaction was continued at room temperature. Upon completion of the reaction, the acetonitrile was removed by concentration and the residue was dissolved in 400 ml of dichloromethane and washed sequentially with 200 ml of dichloromethane, 100 ml of 1N hydrochloric acid and 200 ml of saturated brine solution to remove the tri-n-butylamine hydrochloride and an excess of tri-n-butylamine. After drying, 139 g of white solid was obtained, which was recrystallized by mixed solvent of ethyl acetate and petroleum ether to give a final white solid of 104.2 g. The HPLC purity was 98.9% and the yield was 69.1%.

References

[1] Patent: CN105037237, 2017, B. Location in patent: Paragraph 0075-0078

GMBS Preparation Products And Raw materials

Raw materials

Preparation Products

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GMBS Suppliers

J & K SCIENTIFIC LTD.
Tel
18210857532; 18210857532
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
Advantage
76
Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
4006356688 18621169109
Fax
86-21-61259102
Email
market03@meryer.com
Country
China
ProdList
40228
Advantage
62
3B Pharmachem (Wuhan) International Co.,Ltd.
Tel
821-50328103-801 18930552037
Fax
86-21-50328109
Email
3bsc@sina.com
Country
China
ProdList
15838
Advantage
69
TCI (Shanghai) Development Co., Ltd.
Tel
021-67121386
Fax
021-67121385
Email
Sales-CN@TCIchemicals.com
Country
China
ProdList
24529
Advantage
81
Beijing HwrkChemical Technology Co., Ltd
Tel
18515581800 18501085097
Fax
010-89508210
Email
sales.bj@hwrkchemical.com
Country
China
ProdList
9400
Advantage
55
Energy Chemical
Tel
021-021-58432009 400-005-6266
Fax
021-58436166
Email
sales8178@energy-chemical.com
Country
China
ProdList
44801
Advantage
61
Suzhou Highfine Biotech Co., Ltd
Tel
0512-69209928 18796809688
Fax
0512-68417696
Email
zhouyingxiang@highfine.com
Country
China
ProdList
475
Advantage
75
Nanjing Habo Medical Technology Co., Ltd.
Tel
025-85760892 13376080562
Fax
025-85766769
Email
sales@habotech.com
Country
China
ProdList
884
Advantage
60
Chemsky(shanghai)International Co.,Ltd.
Tel
021-50135380
Email
shchemsky@sina.com
Country
China
ProdList
32321
Advantage
50
Tianjin heowns Biochemical Technology Co., Ltd.
Tel
400 638 7771
Email
sales@heowns.com
Country
China
ProdList
14435
Advantage
57
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View Lastest Price from GMBS manufacturers

Henan Aochuang Chemical Co.,Ltd.
Product
GMBS 80307-12-6
Price
US $0.00-0.00/KG
Min. Order
1KG
Purity
98%
Supply Ability
1ton
Release date
2022-09-26
Zhuozhou Wenxi import and Export Co., Ltd
Product
GMBS 80307-12-6
Price
US $15.00-10.00/KG
Min. Order
1KG
Purity
99%+ HPLC
Supply Ability
Monthly supply of 1 ton
Release date
2021-07-10
Zhuozhou Wenxi import and Export Co., Ltd
Product
GMBS 80307-12-6
Price
US $15.00-10.00/KG
Min. Order
1KG
Purity
99%+ HPLC
Supply Ability
Monthly supply of 1 ton
Release date
2021-07-08

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