CW069 Property

Boiling point:

666.0±55.0 °C(Predicted)

Density 

1.559±0.06 g/cm3(Predicted)

storage temp. 

Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

solubility 

≥50 mg/mL in DMSO; insoluble in H2O; ≥51.6 mg/mL in EtOH

form 

solid

pka

2.69±0.10(Predicted)

color 

White to off-white

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

CW069 Chemical Properties,Usage,Production

Description

CW069 is an allosteric inhibitor of KIFC/HSET (IC₅₀ = 75 μM), a microtubule motor protein involved in bipolar spindle assembly and centrosome clustering. It increases the number of multipolar spindles in N1E-115 mouse neuroblastoma cells with supernumerary centrosomes when used at concentrations of 100 and 200 μM, without affecting the morphology of mitotic spindles in normal human dermal fibroblast (NHDF) cells. CW069 inhibits proliferation of N1E-115, but not NHDF, cells (IC₅₀s = 86 and 181 μM, respectively).

Uses

CW 069 is an allosteric inhibitor of the microtubule motor protein HSET which induces multipolar mitoses only in cells containg supernumerary centrosomes.

References

[1]. watts ca, richards fm, bender a, et al. design, synthesis, and biological evaluation of an allosteric inhibitor of hset that targets cancer cells with supernumerary centrosomes. chem biol, 2013, 20(11): 1399-1410.