ChemicalBook > CAS DataBase List > Fluorescein diacetate

Fluorescein diacetate

Product Name
Fluorescein diacetate
CAS No.
596-09-8
Chemical Name
Fluorescein diacetate
Synonyms
FDA;Mfcd5062;Nsc 4726;Nsc 667259;Diacetylfluor;-Cellstain-Fda;fluoresceinacetate;DIACETYLFLUORESCEIN;3,6-DIACETOXYFLUORAN;Flomoxef Impurity 45
CBNumber
CB5305799
Molecular Formula
C24H16O7
Formula Weight
416.38
MOL File
596-09-8.mol
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Fluorescein diacetate Property

Melting point:
200-203 °C(lit.)
Boiling point:
494.73°C (rough estimate)
Density 
1.2738 (rough estimate)
refractive index 
1.6810 (estimate)
storage temp. 
−20°C
solubility 
acetone: soluble25mg/mL
form 
Crystalline Powder
pka
6.4(at 25℃)
color 
Light yellow
PH Range
Weak green uorescence (6.0) to strong green uorescence (7.2)
Water Solubility 
Soluble in chloroform, DMSO, ethanol, and water.
λmax
490nm
BRN 
62575
Major Application
LED devices, petroleum markers, identifying cancerous cells, detecting bacteria, virus, mitochondrial function in neurons, target molecules, living cells, viable Giardia cysts in water samples, apoptosis, evaluating live/dead state of algae, measurement of totalmicrobial activity in soil, treating ischemia
Biological Applications
Assessing membrane integrity/permeability;43–50 detecting/counting microorganisms; measuring microbial activity; as a substrate for measuring acylases activity, amidases activity,aminopeptidases activity, arylsulfatases activity,chymotrypsin activity, esterases activity;galactosidases activity, glucosidases activity, lipases activity, phosphatases activity, proteases activity; cell viability assay; cytotoxicity assay; implantable medical devices; as temperature sensor; treating ischemic disease
CAS DataBase Reference
596-09-8(CAS DataBase Reference)
EPA Substance Registry System
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)- (596-09-8)
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
24/25-36/37/39-27-26
WGK Germany 
3
TSCA 
Yes
HS Code 
32129000
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P337+P313IF eye irritation persists: Get medical advice/attention.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
343209
Product name
Fluorescein Diacetate - CAS 596-09-8 - Calbiochem
Packaging
5G
Price
$111
Updated
2024/03/01
Sigma-Aldrich
Product number
1182000
Product name
Diacetylfluorescein
Purity
United States Pharmacopeia (USP) Reference Standard
Packaging
200mg
Price
$436
Updated
2024/03/01
TCI Chemical
Product number
F0240
Product name
Fluorescein Diacetate
Purity
>98.0%(HPLC)
Packaging
5g
Price
$80
Updated
2024/03/01
Sigma-Aldrich
Product number
F7378
Product name
Fluorescein diacetate
Purity
used as cell viability stain
Packaging
5g
Price
$87.5
Updated
2024/03/01
Sigma-Aldrich
Product number
F7378
Product name
Fluorescein diacetate
Purity
used as cell viability stain
Packaging
10g
Price
$163
Updated
2024/03/01
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Fluorescein diacetate Chemical Properties,Usage,Production

Description

Fluorescein diacetate (FDA) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, this AM ester yields fluorescein.

Chemical Properties

Light yellow powder

Uses

Fluorescein diacetate is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA. It has been used for the differentiation of live cells from dead cells. It can be also used as a fluorogenic substrate for hGSTP1-1.

Application

Diacetylfluorescein is used in fluorometric staining in the imaging of mammalian cells. Also used in the synthesis of nanocrystal biolabels with releasable fluorophores used in immunoassays. Dyes and metabolites.

Biotechnological Applications

Fluorescein Diacetate Stain is an esterase substrate that can permeate cell membranes. In viable cells with an active metabolism, intracellular esterases hydrolyze Fluorescein Diacetate Stain to produce the green fluorescent compound fluorescein. Fluorescein accumulates in viable cells, causing them to fluoresce green.
Fluorescein Diacetate Stain can be used with Propidium Iodide Stain for Yeast to assess cell viability with the automated fluorescence cell counters of the LUNA® family. Viable cells convert fluorescein diacetate into fluorescein and thus fluoresce green. Nonviable cells take up propidium iodide and fluoresce red.
Fluorescence Signal Enhancer 1 can be used with Fluorescein Diacetate Stain to inhibit ATP-dependent fluorescein efflux from metabolically active cells. This will result in lower background and increased signal intensity, thereby enhancing the fluorescein signal-to-noise ratio.

Fluorescein diacetate Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from Fluorescein diacetate manufacturers

Career Henan Chemical Co
Product
FLUORESCEIN DIACETATE 596-09-8
Price
US $8.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
10MT
Release date
2018-12-19

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  • 3-Oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate
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  • Diacetylfluorescein (200 mg)
  • 3′,6′-diacetyl Uorescein
  • Fluorescein 3′,6′-diacetate
  • Mfcd5062
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  • Nsc 667259
  • FDA [Fluorescein diacetate] *CAS 596-09-8*
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  • Fluorescein Diacetate - CAS 596-09-8 - Calbiochem
  • acetic acid (6'-acetyloxy-3-oxo-3'-spiro[isobenzofuran-1,9'-xanthene]yl) ester
  • 9’-[9H]xanthen]-3-one,3’,6’-bis(acetyloxy)-Spiro[isobenzofuran-1(3H)
  • fluoresceinacetate
  • SUBSTRATE FDA ESTERASE
  • 3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl diacetate
  • FLUORESCEIN DIACETATE, FOR FLUORESCENCE
  • -Cellstain-Fda
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  • 3,6-Diacetoxyfluoran, Di-O-acetylfluorescein
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  • 3',6'-Diacetoxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
  • Bisacetic acid [3'-oxospiro[9H-xanthene-9,1'(3'H)-isobenzofuran]-3,6-diyl] ester
  • Fluorescein diacetate,99%,pure
  • Diacetylfluor
  • Fluoresceinc,diacetate
  • Fluoresceindi-O-acetate(FDA)
  • Spiroisobenzofuran-1(3H),9-9Hxanthen-3-one, 3,6-bis(acetyloxy)-
  • Diacetylfluorescein CRS
  • FluoresceinDiacetate&gt
  • Fluorescein diacetate,97%
  • Flomoxef Impurity 45
  • 3-Oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate
  • 596-09-8
  • C24H16O7
  • Protoplast Isolation and Culture
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  • Plant Biotechnology
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