ChemicalBook > CAS DataBase List > JC-1

JC-1

Product Name
JC-1
CAS No.
3520-43-2
Chemical Name
JC-1
Synonyms
JC-1;VIS515;JC-1, >95%;JC-1 jodide;CBIC2, >95%;JC-1 ，S9784;T4069-5MG JC-1【T4069】;ChemicalbookCBIC2,>95%;JC-1[5,5,6,6-Tetrachloro-1,1,3,3;-tetrachloroimidacarbocyanine iodide
CBNumber
CB5497188
Molecular Formula
C25H27Cl4N4.I
Formula Weight
652.228
MOL File
3520-43-2.mol
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JC-1 Property

Melting point:
275–278 ℃
storage temp. 
room temp
solubility 
Soluble in methanol, N,N-dimethyl formamide, dimethyl sulfoxide
form 
crystals
color 
Deep red
Appearance
Solid Powder
ε(extinction coefficient)
≥175000 at 511-517nm in methanol
λmax
514nm
Stability:
Store as supplied desiccated at -20°C for up to 2 years from the date of purchase. Solutions in DMSO or distilled water may be stored at 4°C for up to 1 year. DO NOT FREEZE/THAW SOLUTIONS. Sterilize solutions only by filtration, not by autoclaving
Biological Applications
Detectingmitochondrialmembrane potential,ABCB1,ABCC1,and ABCG2 transporters inhibitors,nucleic acid hybridization,prostate cancer; treating cellular death,Alzheimer’s disease; apoptosis assay; cytotoxicity assay; hematotoxicity
Major Application
Langmuir-Blodgett films; lasing systems; nonlinear optical materials; photographic materials1,
InChI
InChI=1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1
InChIKey
FYNNIUVBDKICAX-UHFFFAOYSA-M
SMILES
C12C=C(C(Cl)=CC=1N(/C(=C\C=C\C1=[N+](CC)C3C=C(Cl)C(Cl)=CC=3N1CC)/N2CC)CC)Cl.[I-]
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Safety

WGK Germany 
3
F 
10-21
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

H410Very toxic to aquatic life with long lasting effects

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P273Avoid release to the environment.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P321Specific treatment (see … on this label).

P330Rinse mouth.

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P362Take off contaminated clothing and wash before reuse.

P391Collect spillage. Hazardous to the aquatic environment

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
T4069
Product name
JC-1
Purity
solid
Packaging
5mg
Price
$655
Updated
2025/07/31
Cayman Chemical
Product number
15003
Product name
JC-1
Purity
≥90%
Packaging
1mg
Price
$68
Updated
2024/03/01
Cayman Chemical
Product number
15003
Product name
JC-1
Purity
≥90%
Packaging
5mg
Price
$302
Updated
2024/03/01
Cayman Chemical
Product number
15003
Product name
JC-1
Purity
≥90%
Packaging
10mg
Price
$535
Updated
2024/03/01
TRC
Product number
J211955
Product name
JC-1iodide
Packaging
2.5mg
Price
$275
Updated
2021/12/16
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JC-1 Chemical Properties,Usage,Production

Description

JC-1 is a membrane-permeable cationic dye that is used to study mitochondrial integrity in the context of cellular apoptosis. It selectively enters mitochondria and changes fluorescence characteristics with alteration in mitochondrial transmembrane potential (ΔΨm). In healthy cells with a high mitochondrial ΔΨm, JC-1 forms complexes known as J-aggregates, which fluoresce red/orange and may be detected using FL2 settings by flow cytometry. A drop in ΔΨm, a very early event in apoptosis, results in JC-1 monomers, which fluoresce green (FL1 settings on flow cytometers). JC-1 may also be used with fluorescent microscopes or plate readers, using excitation at 520-570 nm and emission at 570-610 nm for J-aggregates and excitation at 485 nm and emission at 535 nm for monomers.

Uses

A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent "J-aggregates," which exhibit broad exc

Uses

The cyanine dye JC-1 has been used to determine mitochondrial transmembrane potential.

Definition

ChEBI: 1,1',3,3'-tetraethyl-5,5',6,6'-tetrachloroimidacarbocyanine iodide is a cyanine dye and an organic iodide salt. It has a role as a fluorochrome. It contains a 1,1',3,3'-tetraethyl-5,5',6,6'-tetrachloroimidacarbocyanine.

References

[1] MARTIN REERS  Lan B C  Thomas W Smith. J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential[J]. Biochemistry Biochemistry, 1991, 30 18: 4480-4486. DOI:10.1021/bi00232a015
[2] S T SMILEY. Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1.[J]. Proceedings of the National Academy of Sciences of the United States of America, 1991, 88 9: 3671-3675. DOI:10.1073/pnas.88.9.3671
[3] F DI LISA. Mitochondrial membrane potential in single living adult rat cardiac myocytes exposed to anoxia or metabolic inhibition.[J]. Journal of Physiology-London, 1995, 486 1: 1-13. DOI:10.1113/jphysiol.1995.sp020786
[4] J MARIO WOLOSIN  Zheng W  Aldo Zamudio. Application of JC1 for non-toxic isolation of cells with MDR transporter activity by flow cytometry.[J]. PLoS ONE, 2017: e0174905. DOI:10.1371/journal.pone.0174905
[5] SHUO WANG. A novel cytoprotective peptide protects mesenchymal stem cells against mitochondrial dysfunction and apoptosis induced by starvation via Nrf2/Sirt3/FoxO3a pathway[J]. Journal of Translational Medicine, 2017, 15 1. DOI:10.1186/s12967-017-1144-5

Excitation / Emission Maximum

485 nm (monomer), 520-570 nm (aggregate)/535 nm (monomer), 570-610 nm (aggregate)

JC-1 Preparation Products And Raw materials

Raw materials

Preparation Products

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JC-1 Suppliers

China 167 Switzerland 2 Ukraine 2 United States 12 Global 183
Lianyungang City and Rui Technology Co., Ltd.
Tel
015162260526; 15162260526
Fax
051885593298
Email
sales@henrychem.com
Country
China
ProdList
300
Advantage
58
Nantong Hi-Future Biotechnology Co., Ltd
Tel
18051384581
Email
sales@chemhifuture.com
Country
China
ProdList
2998
Advantage
58
Guangdong Prochem Biotechnology Co. , Ltd.
Tel
4000707101
Email
research@prochembio.com
Country
China
ProdList
133
Advantage
58
Wuhan EnkiLife Science&Technology Co., Ltd.
Tel
18755910559
Email
order@enkilife.cn
Country
China
ProdList
8413
Advantage
58
Beijing Ouhe Technology Co., Ltd
Tel
010-010-82967028 13522913783
Fax
+86-10-82967029
Email
2355560935@qq.com
Country
China
ProdList
12000
Advantage
60
Dalian Meilun Biotech Co., Ltd.
Tel
0411-62910999 13889544652
Email
sales@meilune.com
Country
China
ProdList
4747
Advantage
58
Nanjing Asian Chemical Co., Ltd.
Tel
+86 (25) 82263158
Fax
+86 (25) 82263168
Country
China
ProdList
262
Advantage
60
ShangHai YuanYe Biotechnology Co., Ltd.
Tel
021-61312847 13636370518
Fax
021-55068248
Email
shyysw007@163.com
Country
China
ProdList
4999
Advantage
60
Haoyuan Chemexpress Co., Ltd.
Tel
021-58950125
Fax
(86) 21-58955996
Email
info@chemexpress.com
Country
China
ProdList
7552
Advantage
61
Shanghai Aladdin Bio-Chem Technology Co.,LTD
Tel
400-6206333 13167063860
Fax
021-50323701
Email
anhua.mao@aladdin-e.com
Country
China
ProdList
25003
Advantage
65
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View Lastest Price from JC-1 manufacturers

Career Henan Chemical Co
Product
JC-1 3520-43-2
Price
US $1.00/KG
Min. Order
1KG
Purity
95--99%
Supply Ability
1ton
Release date
2020-01-03

3520-43-2, JC-1Related Search:

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  • (47729-63-5) 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine iodide
  • CBIC2, >95%
  • JC-1, >95%
  • 5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine iodide
  • JC-1
  • JC-1,5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide, 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide, CBIC2(3)
  • JC-1 jodide
  • 1h-benzimidazolium, 5,6-dichloro-2[3-(5,6-dichloro-1,3-diethyl-1,3-dihydro-2h-benzimidazol-2-ylidene)-1-propenyl]-1,3-diethyl-, Iodide
  • 5,6-dichloro-2-[3-(5,6-dichloro-1,3-diethyl-2-benzimidazolinylidene)propenyl]-1,3-diethylbenzimidazolium Iodide
  • Benzimidazolium, 5,6-dichloro-2-[3-(5,6-dichloro-1,3diethyl-2-benzimidazolinylidene)propenyl]-1,3-diethyl-, Iodide
  • Benzimidazolocarbocyanine Iodide, 5,50,6,60-tetrachloro-1,10,3,30-tetraethyl-
  • Imidacarbocyanine Iodide, 1,10,3,30-tetraethyl-5,50,6,60-tetrachloro-
  • 1,10,3,30-tetraethyl-5,50,6,60-tetrachlorobenzimidazolocarbocyanine Iodide
  • 1,10,3,30-tetraethyl-5,50,6,60-tetrachloroimidacarbocyanine Iodide
  • 5,50,6,60-tetrachloro-1,10,3,30-tetraethylbenzimidazolocarbocyanine Iodide
  • 5,5',6,6'-TETRACHLORO-1,1',3,3'-*TETRAETHYBENZIMIDA
  • 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanine iodide
  • JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2*
  • 5,6-dichloro-2-[(E)-3-(5,6-dichloro-1,3-diethylbenzimidazol-3-ium-2-yl)prop-2-enylidene]-1,3-diethylbenzimidazole
  • -tetrachloroimidacarbocyanine iodide
  • ChemicalbookCBIC2,>95%
  • JC-1[5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide]
  • JC-1[5,5,6,6-Tetrachloro-1,1,3,3
  • inhibit,CBIC-2,JC1,CBIC 2,JC 1,JC-1,Inhibitor
  • VIS515
  • JC-1 mitochondrial membrane potential fluorescent probe
  • JC-1, Mitochondrial membrane potential dye
  • JC-1 [5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide] - 5 mg
  • JC-1 [5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide] - 5 mg
  • JC-1 ，S9784
  • T4069-5MG JC-1【T4069】
  • JC-1 (Mitochondrial Membrane Potential Probe)
  • 3520-43-2
  • Cyanine