Cyanine5 azide

Cyanine5 azide Property

solubility 

Soluble in DMSO, DMF, DCM

form 

Solid

color 

Light brown to brown

Appearance

Dark blue solid

ex/em

640/664 nm (PBS buffer)

ε(extinction coefficient)

250000 L⋅mol−1⋅cm−1

Φ(quantum yield)

0.2

Hazard and Precautionary Statements (GHS)

Cyanine5 azide Chemical Properties,Usage,Production

Description

Cy5 azide is a reactive dye containing an azide group which can participate in Click-Chemistry reactions. Cyanine5 is suitable for many different methods of fluorescence detection and can be easily detected in small quantities (nM) by naked human eye.

Chemical Properties

Appearance: Dark blue solid
ex/em: 640/664 nm (PBS buffer)
Extinction coefficient (ε): 250000 L??mol??1??cm??1
Quantum yield (Φ): 0.2

Uses

Cyanine5 azide chloride is a potent fluorescent dye. Cyanine5 azide chloride can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). (λex=646 nm, λem=662 nm)[1]. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.

References

[1] Huppelschoten Y, et, al. Total Chemical Synthesis of a Functionalized GFP Nanobody. Chembiochem. 2022 Aug 3:e202200304. DOI:10.1002/cbic.202200304

Abs/Em Maxima

646/662 nm

Fluoresene quantum yield

0.2

Extinction Coefficient

250000

CF260

0.03

CF280

0.04