ChemicalBook > CAS DataBase List > 3,3'-Diaminobenzidine tetrahydrochloride hydrate

3,3'-Diaminobenzidine tetrahydrochloride hydrate

Product Name
3,3'-Diaminobenzidine tetrahydrochloride hydrate
CAS No.
868272-85-9
Chemical Name
3,3'-Diaminobenzidine tetrahydrochloride hydrate
Synonyms
DAB，hydrate;DAB·4HCl Hydrate;Diaminobenzidine-4HCl-xH2O;DAB TETRAHYDROCHLORIDE HYDRATE;FLUORESCENT PROTEIN QUANTITATION KIT;3,4-DiaMonobenzidine tetrahydrchloride;RNA LOADING BUFFER WITH ETHIDIUM BROMIDE;3,3-Diaminobenzidine Tetrahydrochloride Hydrate;3,3'-DIAMINOBENZIDINE TETRAHYDROCHLORIDE HYDRATE;3,3μ-Diaminobenzidine hydrate tetrahydrochloride
CBNumber
CB6874674
Molecular Formula
C12H17ClN4O
Formula Weight
268.75
MOL File
868272-85-9.mol
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3,3'-Diaminobenzidine tetrahydrochloride hydrate Property

Melting point:
300°C
storage temp. 
room temp
solubility 
H2O: soluble50mg/mL
form 
Powder
color 
White to Amber to Dark purple
Water Solubility 
Soluble at 5%(w/v) in water
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Safety

Hazard Codes 
Xn,T
Risk Statements 
45-68-36-22
Safety Statements 
22-24/25-45-53-26
WGK Germany 
3
RTECS 
DV8753000
TSCA 
Yes
HS Code 
29215900
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H341Suspected of causing genetic defects

H350May cause cancer

Precautionary statements

P201Obtain special instructions before use.

P202Do not handle until all safety precautions have been read and understood.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P308+P313IF exposed or concerned: Get medical advice/attention.

P405Store locked up.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
32750
Product name
3,3′-Diaminobenzidine tetrahydrochloride hydrate
Purity
for spectrophotometric det. of Se, ≥97.5%
Packaging
5G
Price
$99.8
Updated
2024/03/01
Sigma-Aldrich
Product number
32750
Product name
3,3′-Diaminobenzidine tetrahydrochloride hydrate
Purity
for spectrophotometric det. of Se, ≥97.5%
Packaging
25G
Price
$424
Updated
2024/03/01
Sigma-Aldrich
Product number
32750
Product name
3,3′-Diaminobenzidine tetrahydrochloride hydrate
Purity
for spectrophotometric det. of Se, ≥97.5%
Packaging
1G
Price
$34.4
Updated
2022/05/15
TCI Chemical
Product number
D0078
Product name
3,3'-Diaminobenzidine Tetrahydrochloride Hydrate
Purity
>98.0%(HPLC)(N)
Packaging
5g
Price
$79
Updated
2024/03/01
TCI Chemical
Product number
D0078
Product name
3,3'-Diaminobenzidine Tetrahydrochloride Hydrate
Purity
>98.0%(HPLC)(N)
Packaging
25g
Price
$315
Updated
2024/03/01
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3,3'-Diaminobenzidine tetrahydrochloride hydrate Chemical Properties,Usage,Production

Chemical Properties

Grey to brownish-purple crystalline powder

Uses

3,3'-diaminobenzidine-tetrahydrochloride-dihydrate(DAB) was used as a chromogen, for detecting the antigen-antibody complex. It is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels. It is used as a histological stain for peroxidase activity. Reaction between 3,3'-Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate decarboxylase in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces.

Uses

3,3′-Diaminobenzidine tetrahydrochloride hydrate can be used in HRP-based assays suitable for immunohistology staining procedures, western blotting, and FISH (fluorescence in situ hybridization). DAB is used in studying epithelial cell apoptosis in the event of early cataracts, and immunohistochemistry protocols of autosomal dominant polycystic kidney disease (PKD).

General Description

3,3′-Diaminobenzidine tetrahydrochloride hydrate, also, known as DAB HCl is designed as an immunohistology stain to visualize peroxidase activity in cells. DAB oxidation can be measured using a spectrophotometer.

Biochem/physiol Actions

In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown-colored complex.

3,3'-Diaminobenzidine tetrahydrochloride hydrate Preparation Products And Raw materials

Raw materials

Preparation Products

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3,3'-Diaminobenzidine tetrahydrochloride hydrate Suppliers

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View Lastest Price from 3,3'-Diaminobenzidine tetrahydrochloride hydrate manufacturers

Career Henan Chemical Co
Product
DAB 4HCl 868272-85-9
Price
US $1.00/kg
Min. Order
1kg
Purity
98%
Supply Ability
100KG
Release date
2018-12-16

868272-85-9, 3,3'-Diaminobenzidine tetrahydrochloride hydrateRelated Search:

3,3',4,4'-Biphenyltetramine tetrahydrochloride Xylene N,N,N',N'-Tetramethylethylenediamine Diphenylamine 2,7-Diaminofluorene 3,3'-Diaminobenzidine 3,3'-Diaminobenzidine tetrahydrochloride dihydrate DAB TRIS TABLET 3,4-DIAMINOBIPHENYL DIHYDROCHLORIDE 3,3'-Diaminobenzidine tetrahydrochloride hydrate

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