2-[(4-Bromophenyl)methylene]-N-(2,6-dimethylphenyl)-hydrazinecarboxamide Property

Density 

1.35±0.1 g/cm3(Predicted)

storage temp. 

2-8°C

solubility 

DMSO: soluble5mg/vial, clear (warmed)

form 

powder

pka

11.10±0.46(Predicted)

color 

white to beige

Stability:

Stable for 2 years from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 3 months.

Safety

Hazard Codes 

Xi,N

Risk Statements 

36/37/38-50/53

Safety Statements 

26-36/37/39-60-61

WGK Germany 

3

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

H410Very toxic to aquatic life with long lasting effects

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P273Avoid release to the environment.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P321Specific treatment (see … on this label).

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P362Take off contaminated clothing and wash before reuse.

P391Collect spillage. Hazardous to the aquatic environment

P501Dispose of contents/container to..…

2-[(4-Bromophenyl)methylene]-N-(2,6-dimethylphenyl)-hydrazinecarboxamide Chemical Properties,Usage,Production

Description

EGA (415687-81-9) is a novel selective inhibitor of endosomal trafficking which blocks cellular entry of lethal toxin and other acid-dependent bacterial toxins and viruses into mammalian cells.1?Inhibits Anthrax lethal toxin-induced RAW264.7 cell death, IC50=1.7 mM.1 Delays lysosomal targeting and degradation of the EGF receptor indicating that it targets host membrane trafficking.1 Does not block endosomal recycling of transferrin, retrograde trafficking of ricin, phagolysosomal trafficking or phagosome permeabilization by Franciscella tularensis, nor does it neutralize acidic organelles.1 Protects mammalian cells from Clostridium difficile CDT, Clostridium perfringens Iota toxin and Clostridium botulinum C2 toxin.2 A useful tool for exploring subcellular trafficking of various bacterial toxins.3

Uses

EGA is a selective inhibitor of endosomol trafficking pathways exploited by multiple toxins and viruses.

References

Gillespie et al. (2013), Selective inhibitor of endosomal trafficking pathways exploited by multiple toxins and viruses; Proc. Natl. Acad. Sci. USA, 110 E4904 Schnell et al. (2016), EGA Protects Mammalian Cells from Clostridium difficile CDT, Clostridium perfringens Iota Toxin and Clostridium botulinum C2 Toxin; Toxins (Basel), 8 101 Dixon et al. (2015), Distinct Roles for CdtA and CdtC during Intoxication by Cytolethal Distending Toxins; PLoS One, 10 e0143977