NSC 95397 Property

storage temp. 

-20°C

solubility 

DMSO: 16 mg/mL

form 

Orange solid

color 

orange, solid

Stability:

Stable for 1 year from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 1 month.

Safety

Hazard Codes 

Xi

WGK Germany 

3

HazardClass 

IRRITANT

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H317May cause an allergic skin reaction

H319Causes serious eye irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P272Contaminated work clothing should not be allowed out of the workplace.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P321Specific treatment (see … on this label).

P333+P313IF SKIN irritation or rash occurs: Get medical advice/attention.

P337+P313IF eye irritation persists: Get medical advice/attention.

P363Wash contaminated clothing before reuse.

P501Dispose of contents/container to..…

NSC 95397 Chemical Properties,Usage,Production

Description

NSC 95397 (93718-83-3) is a potent and selective inhibitor of Cdc25 phosphatase (Ki? = 32, 96 and 40 nM for Cdc25A, B and C respectively). NSC-95397 inhibits the growth of several human tumor cell lines and blocks G2/M cell cycle transition.1 Induces cell cycle arrest, phosphorylation of EGFR and activation of ERK-1 and -2.2 Inhibits carboxyl-terminal binding protein a transcriptional corepressor that suppresses multiple pro-apoptotic and epithelial genes.3 Induces eryptosis in human erythrocytes.4

Uses

NSC 95397 is a quinone-based Cdc25 inhibitor, caspase-3 activator, ERK activator, and MKP inhibitor.

Definition

ChEBI: 2,3-bis(2-hydroxyethylthio)naphthalene-1,4-dione is a member of 1,4-naphthoquinones.

Biological Activity

Potent and selective irreversible inhibitor of Cdc25 dual specificity phosphatases (K i values are 32, 96 and 40 nM for inhibition of Cdc25A, -B and -C respectively). Displays 125-180-fold selectivity over VH1-related dual-specificity phosphatase and protein tyrosine phosphatase 1b. Inhibits carcinoma cell growth and blocks G 2 /M phase transition in vitro .

Biochem/physiol Actions

Irreversible Cdc25 dual specificity phosphatase inhibitor.

storage

Desiccate at -20°C

References

1) Lazo et al. (2002), Identification of a potent and selective pharmacophore for Cdc25 dual specificity phosphatase inhibitors; Mol. Pharmacol., 61 720 2) Melchheier et al. (2005), Quinone-induced Cdc25A inhibition causes ERK-dependent connexin phosphorylation; Biochem. Biophys. Res. Commun., 327 1016 3) Blevins et al. (2015), Small Molecule, NSC95397, inhibits the CtBP1-Protein Partner Interaction and CtBP1-Mediated Transcriptional Repression; J. Biomol. Screen., 20 663 4) Jemaa et al. (2016), Stimulation of Suicidal Erythrocyte Death by the CDC25 Inhibitor NSC-95397; Cell. Physiol. Biochem., 40 597