ChemicalBook > CAS DataBase List > 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride

Product Name
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride
CAS No.
25952-53-8
Chemical Name
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride
Synonyms
EDCI;EDC.HCl;EDAC;EDARAVONE;WSC;1-ETHYL-3-(3-DIMETHYLAMINOPROPYL) CARBODIIMIDE;WSCI;WSCD;1-Ethyl-3-(3-dimethyllaminopropyl)carbodiimide hydrochloride;EDC.HCI
CBNumber
CB7403031
Molecular Formula
C8H18ClN3
Formula Weight
191.7
MOL File
25952-53-8.mol
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1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride Property

Melting point:
110-115 °C(lit.)
Density 
0.877 g/mL at 20 °C(lit.)
vapor pressure 
0.002Pa at 20℃
refractive index 
n20/D 1.461
storage temp. 
-20°C
solubility 
H2O: soluble1 gm/10 ml, clear to very slightly hazy, colorless to very faintly yellow
form 
Crystalline Powder
color 
White to off-white
Water Solubility 
Soluble
Sensitive 
Hygroscopic
BRN 
5764110
Stability:
Stable, but sensitive to moisture. Incompatible with strong acids, strong oxidizing agents, moisture.
InChIKey
FPQQSJJWHUJYPU-UHFFFAOYSA-N
LogP
-2.98 at 25℃
CAS DataBase Reference
25952-53-8(CAS DataBase Reference)
EPA Substance Registry System
1,3-Propanediamine, N'-(ethylcarbonimidoyl)-N,N-dimethyl-, monohydrochloride (25952-53-8)
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Safety

Hazard Codes 
C,Xi
Risk Statements 
34-36/37/38-41-37/38-20/21/22
Safety Statements 
26-36/37/39-45-37/39-36
RIDADR 
UN 2735 8/PG 3
WGK Germany 
3
RTECS 
FF2200000
F 
1-3-10
Hazard Note 
Irritant
TSCA 
Y
HS Code 
29252000
Toxicity
LD50 intravenous in mouse: 56mg/kg
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H302Harmful if swallowed

H311Toxic in contact with skin

H315Causes skin irritation

H317May cause an allergic skin reaction

H373May cause damage to organs through prolonged or repeated exposure

H410Very toxic to aquatic life with long lasting effects

Precautionary statements

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P273Avoid release to the environment.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.

P314Get medical advice/attention if you feel unwell.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
E6383
Product name
N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride
Purity
crystalline
Packaging
25G
Price
$570
Updated
2024/03/01
Sigma-Aldrich
Product number
8.00907
Product name
N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride
Purity
forsynthesis
Packaging
500mg
Price
$43.9
Updated
2024/03/01
Sigma-Aldrich
Product number
8.00907
Product name
N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride
Purity
forsynthesis
Packaging
1g
Price
$56.6
Updated
2024/03/01
Sigma-Aldrich
Product number
8.00907
Product name
N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride
Purity
forsynthesis
Packaging
5G
Price
$165
Updated
2024/03/01
Sigma-Aldrich
Product number
8.00907
Product name
N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride
Purity
forsynthesis
Packaging
25g
Price
$343
Updated
2024/03/01
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1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride Chemical Properties,Usage,Production

Chemical Properties

EDC-HCl, or 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, is a white crystalline powder that easily deliquescent and can dissolve in both water and ethanol. It is commonly used as an activating reagent in amide synthesis to activate carboxyl groups. It can also activate phosphate groups, cross-link proteins and nucleic acids, and produce immunocouplers. EDC-HCl is commonly applied in the pH range of 4.0-6.0 and is often used in conjunction with N-Hydroxysuccinimide (NHS) or N-Hydroxysulfosuccinimide sodium salt to improve coupling efficiency.

Uses

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is used as a carboxyl activating agent. It plays a vital role for immobilization of large biomolecules in association with N-hydroxysuccinimide. It is also used in the acylation of phosphoranes.

Application

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is water-soluble carbodiimide, widely used for peptide coupling.
N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used for the formation of FND (fluorescent nanodiamonds)-transferrin bioconjugates.
It has been used for crosslinking polyethylenimine to gold particles.
It has been used as a carbodiimide linkage agent for coating of carboxylated polystyrene beads with biotinylated BSA (bovine serum albumin).

General Description

EDC hydrochloride is a water-soluble derivative of carbodiimide useful for conjugating haptens to proteins and polypeptides. Used to modify NMDA receptors and as a condensing agent in peptide synthesis. The major advantage of EDAC coupling is the easy removal of excess reagent and the corresponding urea by washing with dilute acid or water. Carbodiimides catalyze the formation of amide bonds, carboxylic acids, and amines by activating the carboxylate to form an O-acylurea. This intermediate can be attacked by an amine directly to form an amide. EDAC is released as a soluble urea derivative.

Flammability and Explosibility

Flammable

Biochem/physiol Actions

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride(25952-53-8) is a water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. Additionally, it reacts with phosphate groups. It has been utilized in peptide synthesis, crosslinking proteins to nucleic acids as well as preparation of immunoconjugates.

Mechanism of action

Carboxyl group activation with EDC. xHCl proceeds similarly to other carbodiimide couplings. The reaction of a carboxylic acid and the carbodiimide forms an O-acylisourea intermediate. The O-acylisourea is a highly reactive species that readily reacts with amines, peptide coupling additives, or reducing agents. EDC. HCl is transformed into the corresponding urea during coupling reactions. It has the advantage over DCU in that it can be removed from the reaction mixture by extraction or be washed out from solid phase synthesis applications. However, the O-acylisourea can rearrange irreversibly to an N-acyl urea and racemise the α-carbon of the amino acid via the formation of an oxazol-4(5H)-one [azlactone]. N-Acylurea formation and racemisation may be reduced by using intermediate nucleophiles, which convert the O-acylurea to an activated ester containing the nucleophile. The mechanism of amide bond formation applies both to EDC and EDC.HCl. The differences are that the free base can be applied as a bifunctional reagent: a coupling agent (carbodiimide) and a base (dimethylamino). At the same time, the HCl salt can form an O-acylisourea cyclic intermediate.

Synthesis

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride can be used for the synthesis of amides.It is used as a coupling agent in the synthesis of esters from carboxylic acids using dimethylaminopyridine as the catalyst.
Preparation method of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride(CN104193654A)

Purification Methods

It is an excellent H2O-soluble peptide coupling reagent. It is purified by dissolving (ca 1g) in CH2Cl2 (10mL) at room temperature and then add dry Et2O (~110mL) dropwise and the crystals that separate are collected, washed with dry Et2O, recrystallised from CH2Cl2/Et2O and dried in a vacuum over P2O5. It is important to work in a dry atmosphere or work rapidly and then dry the solid as soon as possible. The material is moderately hygroscopic, but once it becomes wet it reacts slowly with H2O. Store it away from moisture at -20o to slow down the hydrolysis process. The free base has b 47-48o/0.27mm, 53-54o/0.6mm, n 1.4582. The methiodide is recrystallised from CHCl3/EtOAc, the crystals are filtered off, washed with dry Et2O, recrystallised from CHCl3/Et2O, and dried in vacuo over P2O5, m 93-95o, 94-95o. [Sheehan et al. J Am Chem Soc 87 2492 1965, Sheehan & Cruickshank Org Synth Coll Vol V 555 1973.] § A polymer bound version is commercially available.

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride Preparation Products And Raw materials

Raw materials

Preparation Products

2-AMINO-5-BROMOBENZAMIDE 6-BROMO-4-CHLORO-2-METHYL-QUINAZOLINE 6-bromo-2-methylquinazolin-4(3H)-one
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1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride Suppliers

Americas 1 Belgium 3 Canada 7 China 679 Europe 6 France 4 Germany 15 Hungary 1 India 86 Ireland 1 Israel 1 Japan 20 Poland 1 Russia 1 Slovakia 1 South Korea 3 Switzerland 7 United Kingdom 27 United States 80 Global 944
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View Lastest Price from 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride manufacturers

Wuhan Circle Star Chem-medical Technology Co.,Ltd
Product
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride 25952-53-8
Price
US $0.00-0.00/kg
Min. Order
1kg
Purity
98
Supply Ability
1000
Release date
2024-12-26
Jinan Finer Chemical Co., Ltd
Product
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride 25952-53-8
Price
US $0.00/kg
Min. Order
100g
Purity
99%
Supply Ability
100mt
Release date
2019-03-15
Hebei Zhuanglai Chemical Trading Co Ltd
Product
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride 25952-53-8
Price
US $10.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
20 ton
Release date
2024-11-18

25952-53-8, 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochlorideRelated Search:

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  • EDAC · HCl, N-(3-DiMethylaMinopropyl)-N'-ethylcarbodiiMide · HCl, Water-soluble CarbodiiMide, WSC, EDC · HCl
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