3X FLAG PEPTIDE

Product Name: 3X FLAG PEPTIDE
CAS No.: 402750-12-3
Chemical Name: 3X FLAG PEPTIDE
Synonyms: 3×FLAG peptide;3X FLAG PEPTIDE;3X FLAG(R) Peptide;DYKDHDGDYKDHDIDYKDDDDK;MDYKDHDGDYKDHDIDYKDDDDK;3X Flag Peptide Trifluoroacetate;Flag Peptide trifluoroacetate salt;MDYKDHDGDYKDHDIDYKDDDDK (trifluoroacetate salt);MET-ASP-TYR-LYS-ASP-HIS-ASP-GLY-ASP-TYR-LYS-ASP-HIS-ASP-ILE-ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS;3X FLAG peptide,Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys,MDYKDHDGDYKDHDIDYKDDDDK
CBNumber: CB7463716
Molecular Formula: C120H169N31O49S
Formula Weight: 2861.9
MOL File: 402750-12-3.mol

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3X FLAG PEPTIDE Property

Boiling point:: 2863.6±65.0 °C(Predicted)
Density: 1.458±0.06 g/cm3(Predicted)
storage temp.: 2-8°C
solubility: ≥25mg/ml in TBS (0.5M Tris-HCl, pH 7.4, with 1M NaCl), 1X TBS as recommended buffer.
form: lyophilized powder
color: White to off-white
Sequence: Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
InChIKey: SNXSKDGBSPFDDR-SHFSSAJFNA-N

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Safety

WGK Germany: 3

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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: F4799
Product name: 3X FLAG<SUP>®</SUP> Peptide
Purity: lyophilized powder
Packaging: 4 mg
Price: $473
Updated: 2025/07/31

Sigma-Aldrich

Product number: F4799
Product name: 3X FLAG<SUP>®</SUP> Peptide
Purity: lyophilized powder
Packaging: 25 mg
Price: $2200
Updated: 2025/07/31

Sigma-Aldrich

Product number: F4799
Product name: 3X FLAG® Peptide
Purity: lyophilized powder
Packaging: 4MG
Price: $459
Updated: 2024/03/01

Sigma-Aldrich

Product number: F4799
Product name: 3X FLAG® Peptide
Purity: lyophilized powder
Packaging: 25MG
Price: $2130
Updated: 2024/03/01

ApexBio Technology

Product number: A6001
Product name: 3X FLAG Peptide
Packaging: 4mg
Price: $90
Updated: 2021/12/16

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3X FLAG PEPTIDE Chemical Properties,Usage,Production

Uses

3X FLAG peptides are FLAG-tagged peptides containing three repeats of the Asp-Tyr-Lys-Xaa-Xaa-Asp motif. 3X FLAG peptide can be used for protein separation and purification, and competitive elution with target proteins.

Biological Activity

the dykddddk-tag system utilizes a short, hydrophilic 8- amino-acid peptide that is fused to the protein of interest1. the dykddddk peptide binds to the antibody m1. whether binding is calcium-dependent manner2 or –independent3 remains controversial. a disadvantage of the system is that the monoclonalantibody purification matrix is not as stable as others. in general, small tags can be detected with specific monoclonal antibodies.to improve the detection of the dykddddk tag the 3x (dykddddk) system has been developed. this threetandem dykddddk epitope is hydrophilic, 22-amino-acids long and can detect up to 10 fmol of expressed fusion protein. the dykddddk-tagged maltodextrin-binding protein of pyrococcus furiosus has been crystallized4 and the quality of the crystals was very similar to that of crystals of untagged protein.finally, the dykddddk-tag can be removed by treatment with enterokinase, which is specific for the five c-terminal amino acids of the peptide sequence5.

References

1. hopp tp, prickett ks, price vl, libby rt, march cj, ceretti dp, urdal dl, conlon pj (1988) a short polypeptide marker sequence useful for recombinant protein identification and purification. bio/technology 6:1204–1210.2. hopp tp, gallis b, prikett ks (1996) metal-binding properties of a calcium dependent monoclonal antibody. mol immunol 33:601–608.3. einhauer a, jungbauer a (2000) kinetics and thermodynamical properties of the monoclonal antibody m1 directed against the flag peptide. 20th international symposium on the separation of proteins, peptides, and polynucleotides (isppp). lublijana, slovenia, november 5–8, 2000.4. bucher mh, evdokimov ag, waugh ds (2002) differential effects of short affinity tags on the crystallization of pyrococcus furiosus maltodextrin-binding protein. biol cryst 58:392–397.5. maroux s, baratti j, desnuelle p (1971) purification and specificity of procine enterokinase. j biol chem 246:5031–5039.

3X FLAG PEPTIDE Preparation Products And Raw materials

Raw materials

Preparation Products

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3X FLAG PEPTIDE Suppliers

China 99 Switzerland 1 United States 6 Global 106

QYAOBIO (ChinaPeptides CO., Ltd.)

Tel: 021-50792271 18301728382
Email: cps041@chinapeptides.com
Country: China
ProdList: 179
Advantage: 58

YantaiBio

Tel: 13758194781 15083780366
Email: wudegui@tanzhenbio.com
Country: China
ProdList: 1591
Advantage: 58

Shanghai Zhuyan Biotechnology Co., Ltd.

Tel: 17521259211
Email: 17521259211@163.com
Country: China
ProdList: 135
Advantage: 58

Hunan Hui Bai Shi Biotechnology Co., Ltd.

Tel: 0731-85526065 13308475853
Email: ivy@hnhbsj.com
Country: China
ProdList: 7247
Advantage: 62

MedChemexpress LLC

Tel: 021-58955995
Fax: 609-228-5909
Email: sales@medchemexpress.cn
Country: United States
ProdList: 4861
Advantage: 58

ApexBio Technology LLC

Tel: +1-832-696-8203
Fax: +1-832-641-3177
Email: info@apexbt.com
Country: United States
ProdList: 477
Advantage: 60

Wuxi Zhongkun Biochemical Technology Co., Ltd.

Tel: 0510-85629785 18013409632
Fax: 051085625359
Email: sales@reading-chemicals.com
Country: China
ProdList: 15178
Advantage: 58

Shanghai Macklin Biochemical Co.,Ltd.

Tel: 15221275939
Fax: 021-50706099
Email: shenlinxing@macklin.cn
Country: China
ProdList: 15873
Advantage: 55

Creative Peptides

Tel
Email: info@creative-peptides.com
Country: United States
ProdList: 6083
Advantage: 56

Shanghai YuanYe Biotechnology Co., Ltd.

Tel: 021-61312847; 18021002903
Fax: QQ:3008007432
Email: 3008007409@qq.com
Country: China
ProdList: 71826
Advantage: 60

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View Lastest Price from 3X FLAG PEPTIDE manufacturers

RongNa Biotechnology Co.,Ltd

Product: 3X FLAG PEPTIDE 402750-12-3
Price: US $1.00/g
Min. Order: 1g
Purity: 99%
Supply Ability: 100kg
Release date: 2024-03-26

Shandong Hanjiang Chemical Co., Ltd

Product: 3*flag 402750-12-3
Price: US $0.00/kgkg
Min. Order: 1kgkg
Purity: 0.99
Supply Ability: 10T
Release date: 2024-01-11

Career Henan Chemica Co

Product: 3X FLAG PEPTIDE 402750-12-3
Price: US $1.00/KG
Min. Order: 1KG
Purity: 85.0-99.8%
Supply Ability: 20 tons
Release date: 2020-11-09

402750-12-3, 3X FLAG PEPTIDERelated Search:

DNA, SINGLE STRANDED, IMM. ON CELLULOSE, FROM CALF THYMUS* EMP20 GLUCAGON-LIKE PEPTIDE 1 (7-36)-LYS(BIOTIN), AMIDE, HUMAN GLP-2 (RAT) HIV-2 Peptide ALA-ASP-LEU-MET-GLY-TYR-ILE-PRO-LEU-VAL CALCITONIN GENE RELATED PEPTIDE (20-37), HUMAN ALPHA-BAG CELL PEPTIDE (1-9) Calcitonin Gene Related Peptide (CGRP) II, rat CNP-53 (HUMAN)

3X FLAG(R) Peptide

3X FLAG peptide,Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys,MDYKDHDGDYKDHDIDYKDDDDK

MET-ASP-TYR-LYS-ASP-HIS-ASP-GLY-ASP-TYR-LYS-ASP-HIS-ASP-ILE-ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS

3X FLAG PEPTIDE

MDYKDHDGDYKDHDIDYKDDDDK

L-Lysine, L-methionyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-histidyl-L-α-aspartylglycyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-histidyl-L-α-aspartyl-L-isoleucyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-

Flag Peptide trifluoroacetate salt

3×FLAG peptide

3X Flag Peptide Trifluoroacetate

MDYKDHDGDYKDHDIDYKDDDDK (trifluoroacetate salt)

DYKDHDGDYKDHDIDYKDDDDK

402750-12-3

C120H169N31O49S

BioChemical

Protein Purification

Plant Biotechnology

Plant Proteomics

FLAG Affinity Purification

EpitopeTags