CAPSO Property

Melting point:

270-274°C

Density 

1.29±0.1 g/cm3(Predicted)

storage temp. 

Sealed in dry,Room Temperature

solubility 

H₂O: 0.1 g/mL, clear, colorless

form 

Powder

color 

White

PH Range

8.9 - 10.3

pka

9.6(at 25℃)

Odor

Odorless

Water Solubility 

Soluble in water (10 g+ 90 ml).

BRN 

5939234

InChI

InChI=1S/C9H19NO4S/c11-9(7-15(12,13)14)6-10-8-4-2-1-3-5-8/h8-11H,1-7H2,(H,12,13,14)

InChIKey

INEWUCPYEUEQTN-UHFFFAOYSA-N

SMILES

C(S(O)(=O)=O)C(O)CNC1CCCCC1

CAS DataBase Reference

73463-39-5(CAS DataBase Reference)

Safety

Hazard Codes 

Xi,Xn

Risk Statements 

20/21/22-36/37/38

Safety Statements 

22-24/25-36-26

WGK Germany 

3

F 

3

HazardClass 

IRRITANT

HS Code 

29221980

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

CAPSO Chemical Properties,Usage,Production

Description

CAPSO is a useful secondary standard buffer. The second dissociation constant pK2 value of CAPSO has been measured and the useful pH range for CAPSO is 8.9 - 10.3. It is a biological and zwitterionic buffer commonly categorized as a Good's buffer. CAPSO has many applications some of which include immunoblotting and westerns. Additionally, CAPSO is used in the electrotransfer of proteins to PVDF or nitrocellulose membranes. CAPSO buffer has very low metal-binding constants and is, therefore, particularly suited to investigate metal-dependent enzymes. CAPSO is a very water-soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins with minimal salt effects.

Chemical Properties

white powder

Uses

CAPSO was used as biological buffer in the following studies:
To maintain pH 9 during water leach test for the analysis of high-carbon coal fly ash-soil mixtures.
Chemiluminescent in situ hybridization using soybean peroxidase-labeled peptide nucleic acid (PNA) probes.
Preparation of planar bilayer lipid membranes.
It may be employed as buffer for the electrophoretic analyses in non-denaturing gels, to investigate macromolecular self-association of ADP-ribosyltransferase protein in solution.

Uses

CAPSO is used in various biological applications such as electrophoresis. Specific applications include buffers for protein separations and diagnostic reagent production. Also used as biological buffer in the following studies, to maintain pH 9 during water leach test for the analysis of high-carbon coal fly ash-soil mixtures, chemiluminescent in situ hybridization using soybean peroxidase-labeled peptide nucleic acid (PNA) probes, preparation of planar bilayer lipid membranes.

Definition

ChEBI: 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid is an amine.

Preparation

Add sodium sulfite (25.20 g, 0.2 mol) and sodium bisulfite (64.48 g, 0.62 mol) to the three-mouth flask. Heat to dissolve the reactants in water and slowly add epichlorohydrin (50.08 g, 0.54 mol) to the bottle. After vigorous stirring at 30 °C for 2 h, the mixture is cooled in an ice bath. CAPSO is obtained by vacuum suction, drying, and recrystallization.

General Description

CAPSO is a useful secondary standard buffer. Second dissociation constant pK₂ value of CAPSO has been measured. Useful pH range for CAPSO is 8.9 - 10.3.