N-[[(3-Chloro-2-hydroxy-5-nitrophenyl)amino]thioxomethyl]benzamide Property

Density 

1.592

storage temp. 

Store at +4°C

solubility 

DMSO: ≥14mg/mL

form 

powder

pka

4.44±0.50(Predicted)

color 

white to tan

Stability:

Stable for 1 year from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 2 months.

Safety

Hazard Codes 

Xn,N

Risk Statements 

22-50

Safety Statements 

61

RIDADR 

UN 3077 9 / PGIII

WGK Germany 

3

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

H400Very toxic to aquatic life

Precautionary statements

P273Avoid release to the environment.

N-[[(3-Chloro-2-hydroxy-5-nitrophenyl)amino]thioxomethyl]benzamide Chemical Properties,Usage,Production

Description

PtdIns-(3,4,5)-P₃ (PIP₃) serves as an anchor for the binding of signal transduction proteins bearing pleckstrin homology (PH) domains such as phosphatidylinositol 3-kinase (PI3K) or PTEN. Protein binding to PIP₃ is important for cytoskeletal rearrangement and membrane trafficking and initiates an intricate signaling cascade that has been implicated in cancer. PIT-1 is a selective nonphosphoinositide inhibitor that specifically disrupts PIP₃/Akt PH domain binding with an IC₅₀ value of 31 μM. PIT-1 suppresses PI3K-PDK1-Akt-dependent phosphorylation, which has been shown to reduce cell viability and induce apoptosis in PTEN-deficient U87MG glioblastoma cells (IC₅₀ = 37 μM).

Uses

PIT 1 is an apoptosis inducing selective PIP3 antagonist.

References

1) Maio?et al.?(2010),?Small molecule inhibition of phosphatidylinositol-3,4,5-triphosphate (PI3) binding to pleckstrin homology domains; Proc. Natl. Acad. Sci. USA,?107?20126 2) McNamare and Degterev?(2011),?Small-molecule inhibitor of the PI3K signaling network; Future Med. Chem.,?3?549