8-Anilino-1-naphthalenesulfonic acid
- Product Name
- 8-Anilino-1-naphthalenesulfonic acid
- CAS No.
- 82-76-8
- Chemical Name
- 8-Anilino-1-naphthalenesulfonic acid
- Synonyms
- ANS;PHENYL PERI ACID;1,8-ANS;ANSA;1-ANILINONAPHTHALENE-8-SULFONIC ACID;PHENYL ACID;ANSA/1,8-ANS;N-PHENYL PERI ACID;fluorescent probes;PHENYL PERI ACID AND SALTS
- CBNumber
- CB8289935
- Molecular Formula
- C16H13NO3S
- Formula Weight
- 299.34
- MOL File
- 82-76-8.mol
8-Anilino-1-naphthalenesulfonic acid Property
- Melting point:
- 215-217 °C (dec.)(lit.)
- Density
- 1.409±0.06 g/cm3(Predicted)
- storage temp.
- Sealed in dry,Room Temperature
- solubility
- DMF: soluble50mg/mL
- pka
- 0.19±0.40(Predicted)
- form
- Powder
- color
- Dark green to brown to black
- Appearance
- Dark Grey to Black Solid
- Merck
- 13,664
- BRN
- 1843887
- InChI
- InChI=1S/C16H13NO3S/c18-21(19,20)15-11-5-7-12-6-4-10-14(16(12)15)17-13-8-2-1-3-9-13/h1-11,17H,(H,18,19,20)
- InChIKey
- FWEOQOXTVHGIFQ-UHFFFAOYSA-N
- SMILES
- C1(S(O)(=O)=O)=C2C(C=CC=C2NC2=CC=CC=C2)=CC=C1
- CAS DataBase Reference
- 82-76-8(CAS DataBase Reference)
- EPA Substance Registry System
- 8-Anilino-1-naphthalenesulfonic acid (82-76-8)
Safety
- Hazard Codes
- Xi
- Risk Statements
- 36-36/37/38
- Safety Statements
- 26-36
- WGK Germany
- -
- TSCA
- Yes
- HS Code
- 29214980
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation
- Precautionary statements
-
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P405Store locked up.
N-Bromosuccinimide Price
- Product number
- A1028
- Product name
- 8-Anilino-1-naphthalenesulfonic acid
- Purity
- ≥97% (HPLC)
- Packaging
- 5g
- Price
- $66.4
- Updated
- 2025/07/31
- Product number
- A1028
- Product name
- 8-Anilino-1-naphthalenesulfonic acid
- Purity
- ≥97% (HPLC)
- Packaging
- 25g
- Price
- $183
- Updated
- 2025/07/31
- Product number
- A0472
- Product name
- 8-Anilino-1-naphthalenesulfonic Acid
- Purity
- >95.0%(HPLC)(T)
- Packaging
- 25g
- Price
- $81
- Updated
- 2025/07/31
- Product number
- 10010219
- Product name
- 1,8-ANS
- Purity
- ≥98%
- Packaging
- 25000mg
- Price
- $86
- Updated
- 2024/03/01
- Product number
- A1028
- Product name
- 8-Anilino-1-naphthalenesulfonic acid
- Purity
- ≥97% (HPLC)
- Packaging
- 100g
- Price
- $499
- Updated
- 2025/07/31
8-Anilino-1-naphthalenesulfonic acid Chemical Properties,Usage,Production
Description
1,8-ANS is a fluorescent dye that binds with high affinity to hydrophobic surfaces of proteins. 1,8-ANS has an excitation maximum of 350 nm. It has an emission maximum of 520 nm when free in solution but undergoes a blue shift with an increase in fluorescence intensity when bound to protein; for example, when bound to intestinal fatty acid binding protein (FABP2) it has emission maxima of 468-477 nm. 1,8-ANS binds to low polarity regions of protein surfaces making it well suited for determining the affinity of hydrophobic ligands to their corresponding binding proteins, such as the binding of free fatty acids to FABPs. 1,8-ANS binds to FABP2 with a Kd value of approximately 9.7 μM at 24.5°C.
Chemical Properties
green needles
Uses
Intermediate of dyestuff
Uses
8-Anilino-1-naphthalenesulfonic Acid is used as a fluorescent probe to identify hydrophobic sites. Dyes and metabolites.
Definition
CHEBI:8-anilinonaphthalene-1-sulfonic acid is a naphthalenesulfonic acid that is naphthalene-1-sulfonic acid substituted by a phenylamino group at position 8. It has a role as a fluorescent probe. It is an aminonaphthalene and a naphthalenesulfonic acid.
Biotechnological Applications
8-Anilino-1-naphthalenesulfonic acid is a fluorescent dye with high affinity towards hydrophobic surfaces of proteins. It is useful for determining the affinity of hydrophobic ligands to their corresponding binding proteins. 8-Anilino-1-naphthalenesulfonic acid undergoes a blue shift and fluorescence increases when it binds to non-polar regions of a protein surface or membranes. Used as a probe to monitor protein-folding.
References
[1] ALEXANDER V PASTUKHOV Ira J R. Fluorescent dyes as probes to study lipid-binding proteins.[J]. Proteins-Structure Function and Bioinformatics, 2003, 53 3: 607-615. DOI: 10.1002/prot.10401
[2] W R KIRK F G P E Kurian. Characterization of the sources of protein-ligand affinity: 1-sulfonato-8-(1’)anilinonaphthalene binding to intestinal fatty acid binding protein.[J]. Biophysical journal, 1996, 70 1: 69-83. DOI: 10.1016/s0006-3495(96)79592-9
[3] J J ORY L J B. Studies of the ligand binding reaction of adipocyte lipid binding protein using the fluorescent probe 1, 8-anilinonaphthalene-8-sulfonate.[J]. Biophysical journal, 1999, 77 2: 1107-1116. DOI: 10.1016/s0006-3495(99)76961-4
Excitation / Emission Maximum
350 nm (free), 468 nm (bound)/520 nm (free), 477 nm (bound)
8-Anilino-1-naphthalenesulfonic acid Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from 8-Anilino-1-naphthalenesulfonic acid manufacturers
- Product
- 8-Anilino-1-naphthalenesulfonic acid 82-76-8
- Price
- US $10.00/ASSAYS
- Min. Order
- 1ASSAYS
- Purity
- 99%
- Supply Ability
- 1 ton
- Release date
- 2025-08-27
- Product
- 8-Anilino-1-naphthalenesulfonic acid 82-76-8
- Price
- US $0.00-0.00/KG
- Min. Order
- 1KG
- Purity
- 98%
- Supply Ability
- 50kgs/month
- Release date
- 2025-07-24
- Product
- 8-Anilino-1-naphthalenesulfonic acid 82-76-8
- Price
- US $10.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 10 mt
- Release date
- 2024-10-24