ChemicalBook > CAS DataBase List > Hoechst 33258

Hoechst 33258

Product Name
Hoechst 33258
CAS No.
23491-45-4
Chemical Name
Hoechst 33258
Synonyms
BBIH;BXI-72;H33258;abs9129;Ht-33258;hoe33058;NSC334072;HOE 33258;nsc-322921;33258hoechst
CBNumber
CB8329486
Molecular Formula
C25H25ClN6O
Formula Weight
460.97
MOL File
23491-45-4.mol
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Hoechst 33258 Property

Melting point:
314 °C
Density 
1.5099 (rough estimate)
refractive index 
1.6300 (estimate)
storage temp. 
-20°C
solubility 
H2O: 10 mg/mL
form 
powder
color 
Yellow to Very Dark Yellow
Water Solubility 
SOLUBLE
BRN 
4088183
Stability:
Hygroscopic
CAS DataBase Reference
23491-45-4(CAS DataBase Reference)
EPA Substance Registry System
Phenol, 4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]-, trihydrochloride (23491-45-4)
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Safety

Hazard Codes 
Xn
Risk Statements 
22-36/38-36/37/38-20/21/22
Safety Statements 
26-36-24/25-23
WGK Germany 
3
RTECS 
SM1140500
F 
3-8-10
HS Code 
32129000
Toxicity
dog,LD,intravenous,> 22400ug/kg (22.4mg/kg),LUNGS, THORAX, OR RESPIRATION: DYSPNEASENSE ORGANS AND SPECIAL SENSES: LACRIMATION: EYEGASTROINTESTINAL: CHANGES IN STRUCTURE OR FUNCTION OF SALIVARY GLANDS,National Technical Information Service. Vol. PB84-171206,
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
382061
Product name
Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem
Purity
Membrane-permeable, adenine-thymine-specific fluorescent stain.
Packaging
1g
Price
$962
Updated
2024/03/01
Sigma-Aldrich
Product number
14530
Product name
bisBenzimide H 33258
Purity
for fluorescence, ≥98.0% (HPLC)
Packaging
100mg
Price
$105
Updated
2024/03/01
Sigma-Aldrich
Product number
14530
Product name
bisBenzimide H 33258
Purity
for fluorescence, ≥98.0% (HPLC)
Packaging
500mg
Price
$372
Updated
2024/03/01
TCI Chemical
Product number
H1343
Product name
2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride Hydrate[for Biochemical Research]
Purity
>98.0%(HPLC)
Packaging
25mg
Price
$65
Updated
2024/03/01
Cayman Chemical
Product number
16756
Product name
Hoechst 33258
Purity
≥95%
Packaging
50mg
Price
$47
Updated
2024/03/01
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Hoechst 33258 Chemical Properties,Usage,Production

Chemical Properties

yellow to yellow-green powder

Uses

Hoechst 33258 is a Hoechst stain used to stain DNA. It is part of a family of fluorescent dyes. Dyes and metabolites.

Uses

bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry.
Excitation max. =?346 nm
Emission max. =?460 nm

Uses

Hoechst 33258 is useful for staining DNA, chromosomes and nuclei. The nucleic acid binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence

General Description

Membrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca2+ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.

Biochem/physiol Actions

bisBenzimide H 33258 is a potent and selective Bcl-XL inhibitor. bisBenzimide H 33258 is a membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA.

storage

+4°C (desiccate)

Hoechst 33258 Preparation Products And Raw materials

Raw materials

Preparation Products

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Hoechst 33258 Suppliers

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View Lastest Price from Hoechst 33258 manufacturers

Hebei Mojin Biotechnology Co., Ltd
Product
Hoechst 33258 23491-45-4
Price
US $0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
50000KG/month
Release date
2023-09-28
Career Henan Chemical Co
Product
Hoechst 33258 23491-45-4
Price
US $1.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
25kg
Release date
2019-07-12

23491-45-4, Hoechst 33258Related Search:

Bensulfuron methyl Kresoxim-methyl METSULFURON METHYL Cy5.5 Methyl acetate Methylparaben Methyl acrylate Mitochondrial Membrane Potential Assay Kit with JC-1 细胞凋亡－Hoechst染色试剂盒 Hoechst 33342 Thiophanate-methyl Methyl Methyl bromide Parathion-methyl hoechst 33258 (bisbenzimide) HOECHST 33258 TRIHYDROCHLORIDE PENTAHYDRATE,HOECHST 33258, PENTAHYDRATE O-METHACRYLOYL HOECHST 33258 Hoechst 33258

  • Bis-BenzimideFluorochrome
  • p-(5-(5-(4-Methyl-1-piperazinyl)-1H-2-benzimidazolyl)-1H-2-benzimidazolyl)phenol trihydrochloride
  • Hoechst(R) 33258,98%
  • bisBenzimide, 2μ-(4-hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5μ-bi-1H-benzimidazole hydrate trihydrochloride
  • HOE 33258, Hoechst 33258, 2μ-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5μ-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydrochloride
  • 4-[5-[6-(4-Methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1,3-dihydrobenzimidazol-2-ylidene]cyclohexa-2,5-dien-1-one trihydrochloride
  • Phenol, p-(5-(5-(4-methyl-1-piperazinyl)-2-benzimidazolyl)-2-benzimidazolyl)-, trihydrochloride
  • Pibenzimol HCl
  • Ht-33258
  • Bisbenzimide H<sub>3</sub>3258
  • p-(5-(5-(4-Methyl-1-pip
  • bisBenzimide H 33258,2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydroc
  • Bis Benzimide Hoechst H 33258
  • BBIH
  • 2'-(4-Hydroxyphenyl)-5-(4-Methyl-1-piperazinyl)-2,5'-bibenziMidazole Trihydrochloride
  • 2'-(4-Hydroxyphenyl)-5-(4-Methyl-1-piperazinyl)-2,5'-bibenziMidazole Trihydrochloride Hydrate [for BiocheMical Research]
  • Hearst fluorescence fuels, 33258
  • 33258hoechst
  • 4-(5-(4-methyl-1-piperazinyl)(2,5’-bi-1h-benzimidazol)-2’-yl)-phenotrihy
  • 4-[5-(4-methyl-1-piperazinyl)[2,5’-bi-1h-benzimidazol]-2’-yl]-phenotrihydr
  • bisbenzimidazoletrihydrochloride
  • bisbenzimidehydrochloride
  • BISBENZIMIDE H 33258 FLUOROCHROME
  • BISBENZIMIDE H 33258 FLUOROCHROME PACKAG
  • BISBENZIMIDE H 33258, FOR FLUORESCENCE
  • HOECHST 33258,98%
  • BXI-72
  • NSC334072
  • Hoechst 33258 solution
  • Bisbenzimide H33258 Fluorochrome Trihydrochloride 〔HOE 33258〕
  • Bisbenzimide H33258 Fluorochrome Trihydrochloride DMSO Solution (1mg/ml)
  • Bisbenzimide (Hoechst 33258)
  • Hoechst 33258 (trihydrochloride)
  • Hoechst 33258 *20 mM solution in water*
  • Hoechst 33258 3HCl
  • Hoechst 33258 solution, 1mg/mL in H2O
  • Hoechst 33258 *CAS 23491-45-4*
  • Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem
  • 4-[6-(4-Methyl-1-piperazinyl)[2,6'-bi-1H-benzimidazol]-2'-yl]phenol trihydrochloride
  • hoe33058
  • hoechstdye33258
  • nsc-322921
  • HOE 33258
  • HOECHST NO 33258
  • HOECHST STAIN SOLUTION
  • HOECHST 33258
  • H33258
  • BISBENZIMIDE TRIHYDROCHLORIDE
  • BISBENZIMIDE H 33258
  • BISBENZIMIDE H 33258 FLUOROCHROME, TRIHYDROCHLORIDE
  • BISBENZIMIDE H 33258 TRIHYDROCHLORIDE
  • BISBENZIMIDE
  • 2'-(4-HYDROXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5'-BI(1H-BENZIMIDAZOLE) TRIHYDROCHLORIDE
  • 2-[2-(4-HYDROXYPHENYL)-6-BENZIMIDAZOLYL]-6-(1-METHYL-4-PIPERAZYL)-BENZIMIDAZOLE TRIHYDROCHLORIDE
  • 2-[-2-(4-HYDROXYPHENYL)-6-BENZIMIDAZOYL]-6-(1-METHYL-4-PIPERAZYL)-BENZIMIDAZOLE, 3HCL
  • 2-[2-(4-HYDROXYPHENYL)-6-BENZIMIDAZOYL]-6-(1-METHYL-4-PIPERAZYL)BENZIMIDAZOLE TRIHYDROCHLORIDE
  • p-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]phenol trihydrochloride
  • BISBENZIMIDE HOECHST NO 33258TRIHYDROCHL ORIDE