Bicine

Product Name: Bicine
CAS No.: 150-25-4
Chemical Name: Bicine
Synonyms: BICINE BUFFER;N,N-BIS(2-HYDROXYETHYL)GLYCINE;BIClNE;BICINE;Biobuffer;BICINE, 99+%;Fe-3-Specific;Bicine, ULTROL;BICINE BIOXTRA;DIETHYLOLGLYCINE
CBNumber: CB8470568
Molecular Formula: C6H13NO4
Formula Weight: 163.17
MOL File: 150-25-4.mol

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Bicine Property

Melting point:: 190 °C (dec.)(lit.)
Boiling point:: 290.25°C (rough estimate)
Density: 1.05 g/mL at 20 °C
vapor pressure: 0.002-0.007Pa at 20-25℃
refractive index: 1.4240 (estimate)
storage temp.: room temp
solubility: H₂O: 1 M at 20 °C, clear, colorless
form: Powder
color: White
PH: 4.0-5.5 (25℃, 1M in H2O)
pka: 8.3(at 25℃)
PH Range: 7.6 - 9.0
Water Solubility: soluble
λmax: λ: 260 nm Amax: 0.070
λ: 280 nm Amax: 0.040
Merck: 14,1201
BRN: 1769362
LogP: 2.3 at 20℃ and pH7
Surface tension: 72.2mN/m at 1g/L and 20℃
CAS DataBase Reference: 150-25-4(CAS DataBase Reference)
EPA Substance Registry System: Bicine (150-25-4)

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Safety

Hazard Codes: Xn
Risk Statements: 20/21/22-36/37/38
Safety Statements: 24/25-36-26
WGK Germany: 3
RTECS: MB9700000
F: 3-10
TSCA: Yes
HS Code: 29225000
Toxicity: mouse,LD50,intraperitoneal,1540mg/kg (1540mg/kg),KIDNEY, URETER, AND BLADDER: "CHANGES IN TUBULES (INCLUDING ACUTE RENAL FAILURE, ACUTE TUBULAR NECROSIS)",Revue d'Epidemiologie, Medecine Sociale et Sante Publique. Vol. 10, Pg. 391, 1962.

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H332Harmful if inhaled

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 391336-M
Product name: Bicine, ULTROL Grade - CAS 150-25-4 - Calbiochem
Purity: A zwitterionic amino acid buffer active in the pH 7.6-9.0 range. Has a pKa of 8.26 at 25°C. Absorbance (5%, H2O, 250 nm):0.05.
Packaging: 100g
Price: $159
Updated: 2024/03/01

Sigma-Aldrich

Product number: 391336-M
Product name: Bicine, ULTROL Grade - CAS 150-25-4 - Calbiochem
Purity: A zwitterionic amino acid buffer active in the pH 7.6-9.0 range. Has a pKa of 8.26 at 25°C. Absorbance (5%, H2O, 250 nm):0.05.
Packaging: 1kg
Price: $907
Updated: 2024/03/01

Sigma-Aldrich

Product number: RES1151B-B7
Product name: Bicine
Purity: PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production., suitable for cell culture
Packaging: 1pkg
Price: $157
Updated: 2022/05/15

Sigma-Aldrich

Product number: 391336-M
Product name: Bicine, ULTROL® Grade
Purity: A zwitterionic amino acid buffer active in the pH 7.6-9.0 range. Has a pKa of 8.26 at 25°C. Absorbance (5%, H2O, 250 nm):0.05.
Packaging: 391336-100GM
Price: $136
Updated: 2022/05/15

Sigma-Aldrich

Product number: 391336-M
Product name: Bicine, ULTROL® Grade
Purity: A zwitterionic amino acid buffer active in the pH 7.6-9.0 range. Has a pKa of 8.26 at 25°C. Absorbance (5%, H2O, 250 nm):0.05.
Packaging: 391336-1KG
Price: $775
Updated: 2022/05/15

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Bicine Chemical Properties,Usage,Production

Description

Bicine is an amino acid that results from amine degradation due to the presence of oxygen and/or sulfur dioxide. The amine solvents that undergo this degradation are MDEA, DEA, TEA, and mixed amine solvents containing any of these amines. As a zwitterionic amino acid buffer agent, bicine is active in the pH 7.6-9.0 range (pKa of 8.26 at 25°C). Bicine is a recommended buffer for low-temperature biochemical work. It is used for the preparation of stable substrate solution for serum guanase determination. It can be used as a nitrogenous strength enhancer to regulate cement hydration and improve cement properties. Studies have found that bicine significantly changes the morphology of portlandite crystals from a parallel-stacked lamellar shape to a distorted one and also decreases the pore size of the hardened cement paste at an early age[1]. In particular, bicine could promote the rapid formation of β-sheet-rich amyloid-β fibrils[2].

Chemical Properties

Bicine is a white/clear crystalline powder that is somewhat soluble in water. It contains an amino group, carboxyl group, and two hydroxyl units in its structure, making it highly polar. It is often used as a biological buffer and chelating agent.

Uses

To prepare stable substrate solution for the determination of guanine deaminase. The buffer is widely used with the working concentration of 3-100mM.

Uses

A zwitterionic amino acid buffer agent used for low temperature work. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.

Definition

ChEBI: N,N-bis(2-hydroxyethyl)glycine is a bicine that is a Good's buffer substance, pKa = 8.35 at 20 ℃. It is a conjugate acid of a [bis(2-hydroxyethyl)amino]acetate. It is a tautomer of a [bis(2-hydroxyethyl)ammonio]acetate.

Purification Methods

Dissolve bicine in a small volume of hot water and precipitate it with EtOH, twice. Repeat once more but treat the aqueous solution with charcoal Purification of Biochemicals — Amino Acids and Peptides and filter before adding EtOH. Also crystallise it from concentrated aqueous solutions. [Torn & Kolthoff J Am Chem Soc 77 2061 1955, Chaberek et al. J Am Chem Soc 75 2185 1953, Beilstein 4 IV 2390.]

References

[1] Lu X, et al. The influence of bicine on the hydration and properties of Portland cement. Journal of Thermal Analysis and Calorimetry, 2022; 147: 13125–13134.
[2] Kim H, et al. Bicine promotes rapid formation of β-sheet-rich amyloid-β fibrils. PLOS ONE, 2020.

Bicine Preparation Products And Raw materials

Raw materials

Preparation Products

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Bicine Suppliers

Americas 1 Belgium 2 China 336 Czech Republic 1 Europe 5 France 2 Germany 13 India 26 Japan 8 Poland 1 Slovakia 1 South Korea 2 Switzerland 3 The Netherlands 1 Ukraine 1 United Kingdom 20 United States 52 Global 475

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Molekula Ltd

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View Lastest Price from Bicine manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD

Product: Bicine 150-25-4
Price: US $0.00/Kg/Drum
Min. Order: 1KG
Purity: 99%min
Supply Ability: 500kgs
Release date: 2021-08-27

Hebei Weibang Biotechnology Co., Ltd

Product: Bicine 150-25-4
Price: US $25.00/ASSAYS
Min. Order: 100ASSAYS
Purity: 99.5%
Supply Ability: 100 mt
Release date: 2024-11-19

Hebei Chuanghai Biotechnology Co,.LTD

Product: Biobuffer 150-25-4
Price: US $10.00/kg
Min. Order: 1kg
Purity: 98%
Supply Ability: 10 ton
Release date: 2024-08-20

150-25-4, BicineRelated Search:

N,N-Diethylhydroxylamine 2-(METHYLSULFONYL)ETHANOL 4-Hydroxy-D-(-)-2-phenylglycine Betaine 4-Aminophenylacetic acid N,N-Dimethylglycine Glycine ETHANOLDIGLYCINE DISODIUM SALT N,N'-DICARBOXYMETHYLDIAZA-18-CROWN-6, 99+% Ethylenebis(oxyethylenenitrilo)tetraacetic acid N,N-BIS(2-HYDROXYETHYL)GLYCINE SODIUM SALT NITRILOTRIACETIC ACID DISODIUM SALT tripotassium nitrilotriacetate 1,3-DIAMINO-2-PROPANOL-N,N,N',N'-TETRAACETIC ACID Nitrilotriacetic acid NITRILOTRIACETIC ACID TRISODIUM SALT MONOHYDRATE N-(2-HYDROXYETHYL)IMINODIACETIC ACID 2-AMINOOXYETHYLIMINODIACETIC ACID, HYDROCHLORIDE Bicine Buffer

n,n-dihydroxyethyl-glycin

Bicine N,N-Bis(2-hydroxyethyl)glycine Diethanolglycine

N,N-DI(2-HYDROXYETHYL)GLYCINE

N,N-BIS(2-HYDROXYETHYL)GLYCINE

N N-DI(HYDROXYETHYL)-B-AMINOACETIC ACID

BICINE[N,N-Di(2-hydroxyethyl)glycine]

BICIN (N,N-Bis-(2-hydroxyethyl)-glycin)

N,N-Di(2-hydroxyethyl)glycine&nbsp

Bicine, for analysis, 99+% 100GR

Bicine, for biocheMistry, 99+% 100GR

[BIS-(2-HYDROXY-ETHYL)-AMINO]-ACETIC ACID (BICINE)

Bicine SynonyMs Diethylolglycine

PEG Grid Screening Solution 24/Fluka kit no 36436

<i>N,N</i>--Di(2-hydroxyethyl)glycine

BIClNE

MALACHITE GREEN OXALATE HIGH PURITY GR

DI(HYDROXYETHYL)GLYCINE

DIETHYLOLGLYCINE

DIETHANOLGLYCINE

[BIS-(2-HYDROXY-ETHYL)-AMINO]-ACETIC ACID

BICINE

N,N-DIHYDROXYETHYL GLYCINE

BICINE buffer Solution

Bicine, Molecular Biology Grade N,N-Bis(2-hydroxyethyl)glycine, Molecular Biology Grade

Bicine, ULTROL Grade

BICINE BUFFER SOLUTION 1M IN WATER*

BICINE, 99+%

BICINE SIGMAULTRA

BICINE, MOLECULAR BIOLOGY GRADE

Bicine, for analysis, 99+%

BICINE ULTRAPURE

N,N-BIS(2-HYDROXYETHYL)GLYCINE(BICINE)

N,N-Bis(2-hydroxyethyl)glycine >98%

N,N-Di(2-hydroxyethyl)glycine [Good's buffer component for biological research]

2-(Bis(2-hydroxyethyl)amino)acetic acid

N,N-dihydroxyethyl

Bis(2-hydroxyethyl)glycine

Glycine, N,N-bis(2-hydroxyethyl)-

BICINE,BIOLOGICALBUFFER

GLYCINE,N,N-DIHYDROXYETHYL-

Fe-3-Specific

N,N-Bis(2-hydroxyethyl)aminoacetic acid

N,N-Bis(b-hydroxyethyl)glycine

Bicine,99+%,for analysis

BICINE, 99+%, FOR BIOCHEMISTRY

Bicine, for analysis

Bicine, for biochemistry

Bicine, for biochemistry, 99+%

BICINE BUFFER

LABOTEST-BB LT00452695

BicineUltra pure, ≥ 99% (Titration: dried basis)

Bicine, ULTROL

BICINE 30% PEG 600 solution

n,n-bis(2-hydroxyethyl)-glycin

BICINE BIOXTRA

N-bis-(2-Hydroxyethyl)glycine

Bicine, ULTROL Grade - CAS 150-25-4 - Calbiochem

2-(dihydroxyamino)butanoic acid