ChemicalBook > CAS DataBase List > Bicine

Bicine

Product Name
Bicine
CAS No.
150-25-4
Chemical Name
Bicine
Synonyms
BICINE BUFFER;N,N-BIS(2-HYDROXYETHYL)GLYCINE;BIClNE;BICINE;Biobuffer;BICINE, 99+%;Fe-3-Specific;Bicine, ULTROL;BICINE BIOXTRA;DIETHYLOLGLYCINE
CBNumber
CB8470568
Molecular Formula
C6H13NO4
Formula Weight
163.17
MOL File
150-25-4.mol
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Bicine Property

Melting point:
190 °C (dec.)(lit.)
Boiling point:
290.25°C (rough estimate)
Density 
1.05 g/mL at 20 °C
vapor pressure 
0.002-0.007Pa at 20-25℃
refractive index 
1.4240 (estimate)
storage temp. 
room temp
solubility 
H2O: 1 M at 20 °C, clear, colorless
form 
Powder
color 
White
PH
4.0-5.5 (25℃, 1M in H2O)
pka
8.3(at 25℃)
PH Range
7.6 - 9.0
Water Solubility 
soluble
λmax
λ: 260 nm Amax: 0.070
λ: 280 nm Amax: 0.040
Merck 
14,1201
BRN 
1769362
LogP
2.3 at 20℃ and pH7
Surface tension
72.2mN/m at 1g/L and 20℃
CAS DataBase Reference
150-25-4(CAS DataBase Reference)
EPA Substance Registry System
Bicine (150-25-4)
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Safety

Hazard Codes 
Xn
Risk Statements 
20/21/22-36/37/38
Safety Statements 
24/25-36-26
WGK Germany 
3
RTECS 
MB9700000
F 
3-10
TSCA 
Yes
HS Code 
29225000
Toxicity
mouse,LD50,intraperitoneal,1540mg/kg (1540mg/kg),KIDNEY, URETER, AND BLADDER: "CHANGES IN TUBULES (INCLUDING ACUTE RENAL FAILURE, ACUTE TUBULAR NECROSIS)",Revue d'Epidemiologie, Medecine Sociale et Sante Publique. Vol. 10, Pg. 391, 1962.
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H332Harmful if inhaled

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
391336-M
Product name
Bicine, ULTROL Grade - CAS 150-25-4 - Calbiochem
Purity
A zwitterionic amino acid buffer active in the pH 7.6-9.0 range. Has a pKa of 8.26 at 25°C. Absorbance (5%, H2O, 250 nm):0.05.
Packaging
100g
Price
$159
Updated
2024/03/01
Sigma-Aldrich
Product number
391336-M
Product name
Bicine, ULTROL Grade - CAS 150-25-4 - Calbiochem
Purity
A zwitterionic amino acid buffer active in the pH 7.6-9.0 range. Has a pKa of 8.26 at 25°C. Absorbance (5%, H2O, 250 nm):0.05.
Packaging
1kg
Price
$907
Updated
2024/03/01
Sigma-Aldrich
Product number
RES1151B-B7
Product name
Bicine
Purity
PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production., suitable for cell culture
Packaging
1pkg
Price
$157
Updated
2022/05/15
Sigma-Aldrich
Product number
391336-M
Product name
Bicine, ULTROL® Grade
Purity
A zwitterionic amino acid buffer active in the pH 7.6-9.0 range. Has a pKa of 8.26 at 25°C. Absorbance (5%, H2O, 250 nm):0.05.
Packaging
391336-100GM
Price
$136
Updated
2022/05/15
Sigma-Aldrich
Product number
391336-M
Product name
Bicine, ULTROL® Grade
Purity
A zwitterionic amino acid buffer active in the pH 7.6-9.0 range. Has a pKa of 8.26 at 25°C. Absorbance (5%, H2O, 250 nm):0.05.
Packaging
391336-1KG
Price
$775
Updated
2022/05/15
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Bicine Chemical Properties,Usage,Production

Description

Bicine is an amino acid that results from amine degradation due to the presence of oxygen and/or sulfur dioxide. The amine solvents that undergo this degradation are MDEA, DEA, TEA, and mixed amine solvents containing any of these amines. As a zwitterionic amino acid buffer agent, bicine is active in the pH 7.6-9.0 range (pKa of 8.26 at 25°C). Bicine is a recommended buffer for low-temperature biochemical work. It is used for the preparation of stable substrate solution for serum guanase determination. It can be used as a nitrogenous strength enhancer to regulate cement hydration and improve cement properties. Studies have found that bicine significantly changes the morphology of portlandite crystals from a parallel-stacked lamellar shape to a distorted one and also decreases the pore size of the hardened cement paste at an early age[1]. In particular, bicine could promote the rapid formation of β-sheet-rich amyloid-β fibrils[2].

Chemical Properties

Bicine is a white/clear crystalline powder that is somewhat soluble in water. It contains an amino group, carboxyl group, and two hydroxyl units in its structure, making it highly polar. It is often used as a biological buffer and chelating agent.

Uses

To prepare stable substrate solution for the determination of guanine deaminase. The buffer is widely used with the working concentration of 3-100mM.

Uses

A zwitterionic amino acid buffer agent used for low temperature work. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.

Definition

ChEBI: N,N-bis(2-hydroxyethyl)glycine is a bicine that is a Good's buffer substance, pKa = 8.35 at 20 ℃. It is a conjugate acid of a [bis(2-hydroxyethyl)amino]acetate. It is a tautomer of a [bis(2-hydroxyethyl)ammonio]acetate.

Purification Methods

Dissolve bicine in a small volume of hot water and precipitate it with EtOH, twice. Repeat once more but treat the aqueous solution with charcoal Purification of Biochemicals — Amino Acids and Peptides and filter before adding EtOH. Also crystallise it from concentrated aqueous solutions. [Torn & Kolthoff J Am Chem Soc 77 2061 1955, Chaberek et al. J Am Chem Soc 75 2185 1953, Beilstein 4 IV 2390.]

References

[1] Lu X, et al. The influence of bicine on the hydration and properties of Portland cement. Journal of Thermal Analysis and Calorimetry, 2022; 147: 13125–13134.
[2] Kim H, et al. Bicine promotes rapid formation of β-sheet-rich amyloid-β fibrils. PLOS ONE, 2020.

Bicine Preparation Products And Raw materials

Raw materials

Preparation Products

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Bicine Suppliers

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View Lastest Price from Bicine manufacturers

WUHAN FORTUNA CHEMICAL CO., LTD
Product
Bicine 150-25-4
Price
US $0.00/Kg/Drum
Min. Order
1KG
Purity
99%min
Supply Ability
500kgs
Release date
2021-08-27
Hebei Weibang Biotechnology Co., Ltd
Product
Bicine 150-25-4
Price
US $25.00/ASSAYS
Min. Order
100ASSAYS
Purity
99.5%
Supply Ability
100 mt
Release date
2024-11-19
Hebei Chuanghai Biotechnology Co,.LTD
Product
Biobuffer 150-25-4
Price
US $10.00/kg
Min. Order
1kg
Purity
98%
Supply Ability
10 ton
Release date
2024-08-20

150-25-4, BicineRelated Search:

N,N-Diethylhydroxylamine 2-(METHYLSULFONYL)ETHANOL 4-Hydroxy-D-(-)-2-phenylglycine Betaine 4-Aminophenylacetic acid N,N-Dimethylglycine Glycine ETHANOLDIGLYCINE DISODIUM SALT N,N'-DICARBOXYMETHYLDIAZA-18-CROWN-6, 99+% Ethylenebis(oxyethylenenitrilo)tetraacetic acid N,N-BIS(2-HYDROXYETHYL)GLYCINE SODIUM SALT NITRILOTRIACETIC ACID DISODIUM SALT tripotassium nitrilotriacetate 1,3-DIAMINO-2-PROPANOL-N,N,N',N'-TETRAACETIC ACID Nitrilotriacetic acid NITRILOTRIACETIC ACID TRISODIUM SALT MONOHYDRATE N-(2-HYDROXYETHYL)IMINODIACETIC ACID 2-AMINOOXYETHYLIMINODIACETIC ACID, HYDROCHLORIDE Bicine Buffer

  • n,n-dihydroxyethyl-glycin
  • Bicine N,N-Bis(2-hydroxyethyl)glycine Diethanolglycine
  • N,N-DI(2-HYDROXYETHYL)GLYCINE
  • N,N-BIS(2-HYDROXYETHYL)GLYCINE
  • N N-DI(HYDROXYETHYL)-B-AMINOACETIC ACID
  • BICINE[N,N-Di(2-hydroxyethyl)glycine]
  • BICIN (N,N-Bis-(2-hydroxyethyl)-glycin)
  • N,N-Di(2-hydroxyethyl)glycine&nbsp
  • Bicine, for analysis, 99+% 100GR
  • Bicine, for biocheMistry, 99+% 100GR
  • [BIS-(2-HYDROXY-ETHYL)-AMINO]-ACETIC ACID (BICINE)
  • Bicine SynonyMs Diethylolglycine
  • PEG Grid Screening Solution 24/Fluka kit no 36436
  • <i>N,N</i>--Di(2-hydroxyethyl)glycine
  • BIClNE
  • MALACHITE GREEN OXALATE HIGH PURITY GR
  • DI(HYDROXYETHYL)GLYCINE
  • DIETHYLOLGLYCINE
  • DIETHANOLGLYCINE
  • [BIS-(2-HYDROXY-ETHYL)-AMINO]-ACETIC ACID
  • BICINE
  • N,N-DIHYDROXYETHYL GLYCINE
  • BICINE buffer Solution
  • Bicine, Molecular Biology Grade N,N-Bis(2-hydroxyethyl)glycine, Molecular Biology Grade
  • Bicine, ULTROL Grade
  • BICINE BUFFER SOLUTION 1M IN WATER*
  • BICINE, 99+%
  • BICINE SIGMAULTRA
  • BICINE, MOLECULAR BIOLOGY GRADE
  • Bicine, for analysis, 99+%
  • BICINE ULTRAPURE
  • N,N-BIS(2-HYDROXYETHYL)GLYCINE(BICINE)
  • N,N-Bis(2-hydroxyethyl)glycine >98%
  • N,N-Di(2-hydroxyethyl)glycine [Good's buffer component for biological research]
  • 2-(Bis(2-hydroxyethyl)amino)acetic acid
  • N,N-dihydroxyethyl
  • Bis(2-hydroxyethyl)glycine
  • Glycine, N,N-bis(2-hydroxyethyl)-
  • BICINE,BIOLOGICALBUFFER
  • GLYCINE,N,N-DIHYDROXYETHYL-
  • Fe-3-Specific
  • N,N-Bis(2-hydroxyethyl)aminoacetic acid
  • N,N-Bis(b-hydroxyethyl)glycine
  • Bicine,99+%,for analysis
  • BICINE, 99+%, FOR BIOCHEMISTRY
  • Bicine, for analysis
  • Bicine, for biochemistry
  • Bicine, for biochemistry, 99+%
  • BICINE BUFFER
  • LABOTEST-BB LT00452695
  • BicineUltra pure, ≥ 99% (Titration: dried basis)
  • Bicine, ULTROL
  • BICINE 30% PEG 600 solution
  • n,n-bis(2-hydroxyethyl)-glycin
  • BICINE BIOXTRA
  • N-bis-(2-Hydroxyethyl)glycine
  • Bicine, ULTROL Grade - CAS 150-25-4 - Calbiochem
  • 2-(dihydroxyamino)butanoic acid