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Propidium iodide

Product Name
Propidium iodide
CAS No.
25535-16-4
Chemical Name
Propidium iodide
Synonyms
PI;Propidium iodide solution;Nsc169541;PI solution;-Cellstain-Pi;PI(Powder form);PROPIDIUM IODIDE;Propidium diiodide;Propidium Iodide(PI);PI Staining Solution
CBNumber
CB8703712
Molecular Formula
C27H34IN4+
Formula Weight
541.5
MOL File
25535-16-4.mol
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Propidium iodide Property

Melting point:
220-225 °C (dec.) (lit.)
Density 
1.5072 (estimate)
storage temp. 
2-8°C
solubility 
Difficult to mix.
form 
Flaky Powder
color 
Dark red
Water Solubility 
Soluble in water at 1mg/ml
λmax
493 nm, 535 nm
Sensitive 
Light Sensitive & Hygroscopic
BRN 
3843838
Major Application
Electroluminescent displays; photoresists
Biological Applications
Detecting nucleic acids,cancer cells,spores,activity of hydrolase,microorganisms; apoptosis assay; measuring beta-amyloid in Alzheimer’s disease (AD); treating diseases associated with androgens,cancer
CAS DataBase Reference
25535-16-4(CAS DataBase Reference)
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Safety

Hazard Codes 
Xi,Xn
Risk Statements 
36/37/38-68-67-48/20/22-40-36/38-22-20-63
Safety Statements 
26-36-22-36/37
WGK Germany 
3
RTECS 
SF7949600
F 
3-8-10-23
HS Code 
29339900
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H341Suspected of causing genetic defects

Precautionary statements

P201Obtain special instructions before use.

P202Do not handle until all safety precautions have been read and understood.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P308+P313IF exposed or concerned: Get medical advice/attention.

P405Store locked up.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
537059
Product name
Propidium Iodide
Purity
Membrane impermeable DNA intercalator.
Packaging
50mg
Price
$120
Updated
2024/03/01
Sigma-Aldrich
Product number
79214
Product name
Bacteria stain propidium iodide solution
Purity
1?mg/mL in H2O, ready to use solution, 100 assays
Packaging
4X25μL
Price
$140
Updated
2024/03/01
Sigma-Aldrich
Product number
537059
Product name
Propidium Iodide - CAS 25535-16-4 - Calbiochem
Purity
Membrane impermeable DNA intercalator.
Packaging
1g
Price
$1780
Updated
2024/03/01
Sigma-Aldrich
Product number
S7109
Product name
Propidium Iodide Solution
Packaging
1ML
Price
$191
Updated
2022/05/15
Alfa Aesar
Product number
J66764
Product name
Propidium iodide
Packaging
100mg
Price
$191
Updated
2024/03/01
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Propidium iodide Chemical Properties,Usage,Production

Description

Propidium iodide (PI) is membrane impermeant and generally excluded from viable cells. However, it can easily penetrate dead or damaged cells and as such is commonly used for identifying cell viability in a population or as a counterstain in multicolor fluorescent techniques. It binds to DNA and RNA by intercalating between the bases. PI is excited at 488-535 nm with an emission maximum of 617 nm. This probe is suitable for fluorescence microscopy and flow cytometry.

Chemical Properties

Dark Red Solid

Uses

1 mg/mL in water

Uses

Propidium iodide is the most common red-fluorescent nuclear stain. It can also be excited using excitation filters appropriate for green-fluorescent dyes.

Uses

Antibacterial;Detergent

Uses

Used as fluorescent stain for nucleic acids,and used in flow cytometry to evaluate cell viability

Uses

Propidium Iodide is a phenanthridine compound, a class of compounds with reported activity against several species of trypanosomes. Propidium iodide may be used in flow cytometry to evaluate cell viab ility when used with other dyes that stain viable cells or cells that are early in the apoptosis process.

Definition

ChEBI: Propidium iodide is an organic iodide salt. It contains a propidium.

General Description

Extinction coefficient(493 nm): 5900 M -1cm-1;(287 nm): 55,700 M -1cm-1;

Biochem/physiol Actions

Primary TargetMembrane impermeable DNA intercalator

storage

+4°C

Purification Methods

It crystallises as red crystals from H2O containing a little KI. It fluoresces strongly with nucleic acids. [Watkins J Chem Soc 3064 1952, Beilstein 22 III/IV 5519.] TOXIC.

Propidium iodide Preparation Products And Raw materials

Raw materials

Preparation Products

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Propidium iodide Suppliers

Canada 2 China 209 Europe 3 France 1 Germany 4 India 11 Japan 4 Poland 1 Slovakia 1 South Korea 1 Switzerland 4 United Kingdom 11 United States 41 Global 293
Guangdong Prochem Biotechnology Co. , Ltd.
Tel
4000707101
Email
research@prochembio.com
Country
China
ProdList
122
Advantage
58
Wuhan Linglingjiu Biotechnology Co., Ltd
Tel
15623309010
Email
1287744812@qq.com
Country
China
ProdList
1657
Advantage
58
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
94657
Advantage
76
INTATRADE GmbH
Tel
+49 3493/605464
Fax
+49 3493/605470
Email
sales@intatrade.de
Country
Germany
ProdList
3572
Advantage
66
3B Pharmachem (Wuhan) International Co.,Ltd.
Tel
821-50328103-801 18930552037
Fax
86-21-50328109
Email
3bsc@sina.com
Country
China
ProdList
15839
Advantage
69
Beijing HwrkChemical Technology Co., Ltd
Tel
010-89508211 18501085097
Fax
010-89508210
Email
sales.bj@hwrkchemical.com
Country
China
ProdList
8418
Advantage
55
Beijing Ouhe Technology Co., Ltd
Tel
010-82967028 13552068683
Fax
+86-10-82967029
Email
2355560935@qq.com
Country
China
ProdList
12390
Advantage
60
Adamas Reagent, Ltd.
Tel
400-6009262 16621234537
Fax
021-64823266
Email
chenyj@titansci.com
Country
China
ProdList
14103
Advantage
59
Chemsky（shanghai）International Co.,Ltd.
Tel
021-50135380
Email
shchemsky@sina.com
Country
China
ProdList
32321
Advantage
50
Shandong Xiya Chemical Co., Ltd
Tel
4009903999 13355009207
Fax
0539-6365991
Email
3007715519@qq.com
Country
China
ProdList
18738
Advantage
57
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View Lastest Price from Propidium iodide manufacturers

Hebei Weibang Biotechnology Co., Ltd
Product
Propidium iodide 25535-16-4
Price
US $0.00-0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
500000kg
Release date
2024-10-25
Hebei Yanxi Chemical Co., Ltd.
Product
Propidium iodide 25535-16-4
Price
US $20.00-3.00/kg
Min. Order
1kg
Purity
0.99
Supply Ability
20 tons
Release date
2023-09-28
Hebei Mojin Biotechnology Co., Ltd
Product
Propidium iodide 25535-16-4
Price
US $0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
50000KG/month
Release date
2023-09-28

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