ChemicalBook > CAS DataBase List > Propidium iodide

Propidium iodide

Product Name
Propidium iodide
CAS No.
25535-16-4
Chemical Name
Propidium iodide
Synonyms
PI;Propidium iodide solution;PI (Red);Nsc169541;C27H34IN4;PI solution;-Cellstain-Pi;PI(Powder form);PROPIDIUM IODIDE;Propidium diiodide
CBNumber
CB8703712
Molecular Formula
C27H34IN4+
Formula Weight
541.5
MOL File
25535-16-4.mol
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Propidium iodide Property

Melting point:
220-225 °C (dec.) (lit.)
Density 
1.5072 (estimate)
storage temp. 
2-8°C
solubility 
Difficult to mix.
form 
Flaky Powder
color 
Dark red
Appearance
Red solid or powder
Water Solubility 
Soluble in water at 1mg/ml
λmax
493 nm, 535 nm
Sensitive 
Light Sensitive & Hygroscopic
ex/em
535/617 nm (and DNA)
ε(extinction coefficient)
5400 cm−1M−1 (water/DNA)
BRN 
3843838
Major Application
Electroluminescent displays; photoresists
Biological Applications
Detecting nucleic acids,cancer cells,spores,activity of hydrolase,microorganisms; apoptosis assay; measuring beta-amyloid in Alzheimer’s disease (AD); treating diseases associated with androgens,cancer
InChIKey
ISKCJFFZOPXOFC-UHFFFAOYSA-N
SMILES
C([N+]1=C2C=C(N)C=CC2=C2C=CC(N)=CC2=C1C1C=CC=CC=1)CC[N+](C)(CC)CC.[I-]
CAS DataBase Reference
25535-16-4(CAS DataBase Reference)
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Safety

Hazard Codes 
Xi,Xn
Risk Statements 
36/37/38-68-67-48/20/22-40-36/38-22-20-63
Safety Statements 
26-36-22-36/37
WGK Germany 
3
RTECS 
SF7949600
F 
3-8-10-23
HS Code 
29339900
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H341Suspected of causing genetic defects

Precautionary statements

P201Obtain special instructions before use.

P202Do not handle until all safety precautions have been read and understood.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P308+P313IF exposed or concerned: Get medical advice/attention.

P405Store locked up.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
S7109
Product name
Propidium Iodide Solution
Packaging
1ML
Price
$233
Updated
2025/07/31
Sigma-Aldrich
Product number
537059
Product name
Propidium Iodide
Purity
Membrane impermeable DNA intercalator.
Packaging
50mg
Price
$124
Updated
2025/07/31
Sigma-Aldrich
Product number
79214
Product name
Bacteria stain propidium iodide solution
Purity
1?mg/mL in H2O, ready to use solution, 100 assays
Packaging
4X25μL
Price
$145
Updated
2025/07/31
Sigma-Aldrich
Product number
537059
Product name
Propidium Iodide - CAS 25535-16-4 - Calbiochem
Purity
Membrane impermeable DNA intercalator.
Packaging
1g
Price
$1840
Updated
2025/07/31
Cayman Chemical
Product number
14289
Product name
Propidium Iodide
Purity
≥90%
Packaging
10mg
Price
$32
Updated
2024/03/01
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Propidium iodide Chemical Properties,Usage,Production

Description

Propidium iodide (PI) is membrane impermeant and generally excluded from viable cells. However, it can easily penetrate dead or damaged cells and as such is commonly used for identifying cell viability in a population or as a counterstain in multicolor fluorescent techniques. It binds to DNA and RNA by intercalating between the bases. PI is excited at 488-535 nm with an emission maximum of 617 nm. This probe is suitable for fluorescence microscopy and flow cytometry.

Chemical Properties

Dark Red Solid

Uses

1 mg/mL in water

Uses

Propidium iodide is the most common red-fluorescent nuclear stain. It can also be excited using excitation filters appropriate for green-fluorescent dyes.

Uses

Antibacterial;Detergent

Uses

Used as fluorescent stain for nucleic acids,and used in flow cytometry to evaluate cell viability

Uses

Propidium Iodide is a phenanthridine compound, a class of compounds with reported activity against several species of trypanosomes. Propidium iodide may be used in flow cytometry to evaluate cell viab ility when used with other dyes that stain viable cells or cells that are early in the apoptosis process.

Definition

ChEBI: Propidium iodide is an organic iodide salt. It contains a propidium.

General Description

Extinction coefficient(493 nm): 5900 M -1cm-1;(287 nm): 55,700 M -1cm-1;

Biochem/physiol Actions

Primary TargetMembrane impermeable DNA intercalator

storage

+4°C

Purification Methods

It crystallises as red crystals from H2O containing a little KI. It fluoresces strongly with nucleic acids. [Watkins J Chem Soc 3064 1952, Beilstein 22 III/IV 5519.] TOXIC.

References

[1] SIMON JOHNSON  David C  Vy Nguyen. Assessment of Cell Viability[J]. Current Protocols in Cytometry, 2013, 64 1. DOI: 10.1002/0471142956.cy0902s64

Abs/Em Maxima

537 (complex)/616 (complex) nm

Propidium iodide Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from Propidium iodide manufacturers

Hebei Chuanghai Biotechnology Co., Ltd
Product
Propidium iodide 25535-16-4
Price
US $0.00-0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
500000kg
Release date
2024-10-25
Hebei Yanxi Chemical Co., Ltd.
Product
Propidium iodide 25535-16-4
Price
US $20.00-3.00/kg
Min. Order
1kg
Purity
0.99
Supply Ability
20 tons
Release date
2023-09-28
Career Henan Chemical Co
Product
Propidium iodide 25535-16-4
Price
US $7.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
JD 432
Release date
2019-09-01

25535-16-4, Propidium iodideRelated Search:

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