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7-HYDROXYCOUMARIN-4-ACETIC ACID

Product Name
7-HYDROXYCOUMARIN-4-ACETIC ACID
CAS No.
6950-82-9
Chemical Name
7-HYDROXYCOUMARIN-4-ACETIC ACID
Synonyms
NSC 65625;NSC 642907;UMBELLIFERONE-4-ACETIC ACID;B-UMBELLIFERONE ACETIC ACID;4-(CARBOXYMETHYL)UMBELLIFERONE;7-HYDROXYCOUMARIN-4-ACETIC ACID;7-HYDROXYCOUMARINYL-4-ACETIC ACID;7-HYDROXY-4-COUMARINYLACETIC ACID;4- Acotic acid -7- hydroxy coumarin;7-HydroxycouMarinyl-4-acetic acid 97%
CBNumber
CB9213782
Molecular Formula
C11H8O5
Formula Weight
220.18
MOL File
6950-82-9.mol
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7-HYDROXYCOUMARIN-4-ACETIC ACID Property

Melting point:
212 °C (dec.) (lit.)
Boiling point:
523.5±50.0 °C(Predicted)
Density 
1.511±0.06 g/cm3(Predicted)
storage temp. 
Sealed in dry,Room Temperature
solubility 
DMF: soluble
form 
Crystalline Powder
pka
4.24±0.10(Predicted)
color 
Off-white to pale yellow
λmax
326nm(MeOH)(lit.)
BRN 
204777
InChI
InChI=1S/C11H8O5/c12-7-1-2-8-6(3-10(13)14)4-11(15)16-9(8)5-7/h1-2,4-5,12H,3H2,(H,13,14)
InChIKey
BNHPMQBVNXMPDU-UHFFFAOYSA-N
SMILES
C1(=O)OC2=CC(O)=CC=C2C(CC(O)=O)=C1
CAS DataBase Reference
6950-82-9(CAS DataBase Reference)
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-36
WGK Germany 
3
8
HS Code 
29322985
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
335665
Product name
7-Hydroxycoumarinyl-4-acetic acid
Purity
97%
Packaging
1g
Price
$67.6
Updated
2025/07/31
Sigma-Aldrich
Product number
335665
Product name
7-Hydroxycoumarinyl-4-acetic acid
Purity
97%
Packaging
5g
Price
$91.7
Updated
2025/07/31
TCI Chemical
Product number
H1501
Product name
7-Hydroxycoumarin-4-acetic Acid
Purity
>98.0%(HPLC)(T)
Packaging
1g
Price
$48
Updated
2021/12/16
TCI Chemical
Product number
H1501
Product name
7-Hydroxycoumarin-4-acetic Acid
Purity
>98.0%(HPLC)(T)
Packaging
5g
Price
$148
Updated
2022/04/27
TRC
Product number
H825135
Product name
7-Hydroxycoumarin-4-acetic Acid
Packaging
10g
Price
$215
Updated
2021/12/16
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7-HYDROXYCOUMARIN-4-ACETIC ACID Chemical Properties,Usage,Production

Uses

Fluorogenic substrate

Uses

7-Hydroxycoumarinyl-4-acetic acid was used in the synthesis of novel and specific enzyme substrates for Maroteaux-Lamy (MPS VI) and Morquio A (MPS IVA) diseases. It was also employed as ionic liquid matrice for MALDI-TOF MS of proteins, peptides and carbohydrates.

Uses

7-Hydroxycoumarin-4-acetic acid is a fluorescence label but can also be used as pH-indicator.

Synthesis

77-92-9

108-46-3

6950-82-9

GENERAL METHODS: Coumarin-4-acetic acid is a known compound (Manware et al., 2008). The reaction conditions for this compound were modified and optimized (Method A) to synthesize the analogues 2a-2f. These analogues were also synthesized by microwave-assisted methods (Method B). Method A: A mixture of citric acid (0.02 mol) and concentrated sulfuric acid (0.03 mol) was stirred at room temperature for 30 min. The mixture was placed in a boiling water bath to remove carbon monoxide (note: this needs to be done in a fume hood). Once the release of CO gas slowed down, the flask was removed from the water bath and left to stand for 15 minutes or until no more CO bubbles were produced in the reaction mixture. The reaction mixture was cooled to 10 °C and (un)substituted phenol (0.02 mol), pre-cooled to 10 °C, was added dropwise. The reaction mixture was stirred at room temperature for 48 h and then decanted onto ice. The precipitate was filtered and dissolved in saturated sodium bicarbonate solution. The solution was acidified to give intermediates 2a-2f. Method B: A mixture of citric acid (0.02 mol), concentrated sulfuric acid (0.03 mol), and (un)substituted phenol (0.02 mol) was placed in a microwave reactor and heated at 10% power for 4 min. The reaction mixture was poured onto crushed ice and processed according to the subsequent steps of Method A to obtain the target product. Yield of 7-hydroxycoumarin-4-acetic acid (2a): 67% for Method A and 78% for Method B; melting point: 170°C; IR (FT-IR) νmax (cm?1): 3210 (Ar C-H), 2924 (Al C-H), 1710 (C=O), 1701 (lactone C=O), 1458 (C=C), 1130 (C-O) , 1290 (C-OH); 1H-NMR (DMSO-d6, 400 MHz): δ 11.08 (1H, s, COOH), 7.41-6.98 (2H, m, H-5 and H-6), 6.69-6.23 (2H, m, H-8 and H-3), 4.93 (1H, dd, OH), 3.02-2.89 (2H, m , CH2); 13C-NMR (DMSO-d6, 100 MHz): δ 171.5 (C=O), 160.2 (C-2), 112.5 (C-3), 152.4 (C-4), 150.5 (C-8a), 135.2 (C-6), 124.8 (C-5), 122.9 (C-7), 120.1 (C-4a ), 104.1 (C-8), 37.0 (-CH2). HRMS (+ESI) [M+H]+: 221.0445 (calculated value), 221.0464 (measured value).

References

[1] Medicinal Chemistry Research, 2018, vol. 27, # 1, p. 61 - 71
[2] Tetrahedron Letters, 2012, vol. 53, # 48, p. 6598 - 6601,4
[3] Bioorganic and Medicinal Chemistry Letters, 2016, vol. 26, # 23, p. 5732 - 5735
[4] Journal of the Chemical Society, 1950, p. 170,172
[5] Journal of the American Chemical Society, 1950, vol. 72, p. 3987,3989

7-HYDROXYCOUMARIN-4-ACETIC ACID Preparation Products And Raw materials

Raw materials

Preparation Products

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7-HYDROXYCOUMARIN-4-ACETIC ACID Suppliers

9ding chemical ( Shanghai) Limited
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Energy Chemical
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View Lastest Price from 7-HYDROXYCOUMARIN-4-ACETIC ACID manufacturers

Career Henan Chemical Co
Product
7-HYDROXYCOUMARIN-4-ACETIC ACID 6950-82-9
Price
US $500.00/KG
Min. Order
1KG
Purity
98%
Supply Ability
1ton
Release date
2018-08-04

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