DISTAMYCIN A HYDROCHLORIDE Property

Melting point:

184-187° (Arcamone, 1967). Also reported as 189-193° from ethanol-ethyl acetate (Bialer)

storage temp. 

−20°C

Safety

Hazard Codes 

Xi

Risk Statements 

43

Safety Statements 

36/37

WGK Germany 

3

RTECS 

WZ7110000

Toxicity

LD50 in mice (mg/kg): 75 i.v.; 500 i.p. (DiMarco)

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H317May cause an allergic skin reaction

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P272Contaminated work clothing should not be allowed out of the workplace.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P321Specific treatment (see … on this label).

P333+P313IF SKIN irritation or rash occurs: Get medical advice/attention.

P363Wash contaminated clothing before reuse.

P501Dispose of contents/container to..…

DISTAMYCIN A HYDROCHLORIDE Chemical Properties,Usage,Production

Originator

Herperal,Farmitalia,Italy,1978

Manufacturing Process

A spore suspension obtained upon washing a culture of Streptomyces distallicus is added to 3,000 ml of a sterile medium consisting of the following:Dextrose 2% Corn steep liquor extract 2% CaCO3 1% (NH4)2SO4 0.3% NaCl 0.3%Fermentation is continued at 28°C for 40 hours at a stirring rate of 150 to 250 rpm and a rate of air flow of 1 to 2 l/min/l of culture medium.
300 ml of a suspension of the vegetative mycelium of this culture are used for inoculating 6,000 ml of a similar sterile culture medium. At this production stage, the culture is kept fermenting for 85 to 100 hours (pH 7.6 at 28°C) at a stirring rate of 350 to 450 rpm and a rate of air flow of 1 to 1.5 l/min/l of culture medium.
To 17 l of a culture obtained by submerged fermentation as mentioned above, siliceous earth is added and the batch is filtered. The mixture of mycelium and the siliceous earth are agitated for 1 hour with 2.5 l of butanol. This treatment is repeated twice. The butanolic extracts are combined, washed with water, evaporated to dryness (about 10 g) and boiled with acetone (80 ml). The 5 g of distamycin is extracted six times with ethanol. The ethanolic extracts are combined, concentrated and filtered through a column containing 70 g of alumina. Elution is carried out with the same solvent. The effluent (central fractions) is collected and evaporated to dryness to yield 0.43 g of pure distamycin A: decomposition point, 183°C to 185°C. The product can be further purified by crystallization from aqueous n-butanol.

Therapeutic Function

Antibiotic