Cyanine5.5 NHS ester

Cyanine5.5 NHS ester Property

solubility 

soluble in organic solvents (DMSO, DMF, dichloromethane), practically insoluble in water (< 1 uM, < 1 mg/L)

Appearance

dark blue to violet solid

InChIKey

QJHUOARMFFEDJW-UHFFFAOYSA-N

SMILES

[B+3]([F-])([F-])([F-])[F-].CC1(C(N(CCCCCC(=O)ON2C(CCC2=O)=O)C2C=CC3=CC=CC=C3C1=2)=CC=CC=CC1=[N+](C2C=CC3=CC=CC=C3C=2C1(C)C)C)C

Hazard and Precautionary Statements (GHS)

Cyanine5.5 NHS ester Chemical Properties,Usage,Production

Description

Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester.

Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.

This reagent can replace NHS esters of Cy5.5® and DyLight 680.

Uses

Cyanine5.5 NHS ester tetrafluoroborate belongs to the cyanine dye series and is a common fluorescent marker for biomolecules that can interact with biomolecules. Cyanine dyes may also bind to double-helical DNA through intercalation and exhibit enhanced fluorescence upon binding.

References

[1] Armitage B A. Cyanine dye–DNA interactions: intercalation, groove binding, and aggregation[J]. DNA Binders and Related Subjects: -/-, 2005: 55-76.

Abs/Em Maxima

684/710 nm

Fluoresene quantum yield

0.2

Extinction Coefficient

198000

CF260

0.07

CF280

0.03