5-ETHYNYL-2'-DEOXYURIDINE
5-ETHYNYL-2'-DEOXYURIDINE Basic information
- Product Name:
- 5-ETHYNYL-2'-DEOXYURIDINE
- Synonyms:
-
- 5-ETHYNYL-2'-DEOXYURIDINE
- 2’-deoxy-5-ethynyl-uridin
- 2’-deoxy-5-ethynyluridine
- EYdU
- 5-Ethynyl-durd
- Aids072372
- Aids-072372
- 2'deoxyuridine, 5-ethynyl
- CAS:
- 61135-33-9
- MF:
- C11H12N2O5
- MW:
- 252.22
- EINECS:
- 500-100-4
- Product Categories:
-
- SiChem
- Mol File:
- 61135-33-9.mol
5-ETHYNYL-2'-DEOXYURIDINE Chemical Properties
- Melting point:
- 199°C(lit.)
- Density
- 1.55±0.1 g/cm3(Predicted)
- storage temp.
- -20°C
- solubility
- Soluble in DMSO (up to 10 mg/ml).
- pka
- 8.29±0.10(Predicted)
- form
- powder
- color
- White or off-white
- Stability:
- Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20° for up to 2 months.
- InChIKey
- CDEURGJCGCHYFH-DJLDLDEBSA-N
- CAS DataBase Reference
- 61135-33-9
Safety Information
- Hazard Codes
- T
- Risk Statements
- 46-61
- Safety Statements
- 53-45-24/25
- RTECS
- YU7510000
- HS Code
- 29349990
5-ETHYNYL-2'-DEOXYURIDINE Usage And Synthesis
Description
5-Ethynyl-2′-deoxyuridine (61135-33-9) is a novel agent for fast and sensitive detection of DNA synthesis?in vivo.1 5-EdU is incorporated into proliferating cells followed by detection with a fluorescent azide using click ligation.1 In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. 5-Ethynyl-2′-deoxyuridine may be used for studying cell proliferation in the central nervous system and may be combined with BrdU staining in a novel protocol allowing for double labeling of DNA synthesis.2
Uses
5-Ethynyl-2’deoxyuridine is a extensively used nucleoside analog used as a probe for DNA synthesis. However, 5-Ethynyl-2’deoxyuridine is a toxic anti-metabolite, which potentially causes DNA instabi liy, necrosis and cell-cycle arrest, therefore it is often used for short-term DNA synthesis in tissue culture and living organisms where prolonged cell survival is not required.
Uses
5-Ethynyl-2′-deoxyuridine (EdU) is a thymidine analog that can be incorporated into replicating DNA for detection of cell proliferation. It has an alkyne handle that can ligate with azide-containing fluorescent probes through a highly efficient click chemistry reaction. EdU nucleoside can be incorporated into the DNA of cells either in culture or in animals without showing significant toxicity. The assay offers a simpler workflow for detecting cell proliferation than anti-BrdU assays.
For information on Baseclick kits containing all the reagents for the assays see: Baseclick kits
For a listing of the Baseclick kits see: Baseclick kits
storage
Store at -20°C
References
1) Salic and Mitchison (2008),?A chemical method for fast and sensitive detection of DNA synthesis in vivo; Proc. Natl. Acad. Sci. USA,?105?2415 2) Zeng?et al. (2010),?Evaluation of 5-ethynyl-2′-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system; Brain Res.,?1319C?21
5-ETHYNYL-2'-DEOXYURIDINESupplier
- Tel
- 0553-8233716 18055326116
- sales@nuowei-chem.com
- Tel
- 18913822354
- market@geneyan-bio.com
- Tel
- 19308655899
- 2677609105@qq.com
- Tel
- 010-82848833 400-666-7788
- jkinfo@jkchemical.com
- Tel
- 021-64757213
- info@hongene.com
5-ETHYNYL-2'-DEOXYURIDINE(61135-33-9)Related Product Information
- 5-Ethynyl uridine
- Netivudine
- 5-ETHYNYL-2'-DEOXYURIDINE
- FLUORESCEIN-12-DUTP
- FLUORESCEIN-12-UTP
- CHROMATIDE(TM) TEXAS RED(R)-5-DUTP
- DIETHYLAMINOCOUMARIN-5-DUTP
- FLUORESCEIN CHLOROTRIAZINYL-4-UTP
- LISSAMINE-5-UTP
- CHROMATIDE(TM) BODIPY(R) 630/650-14-DUTP
- 5-PROPYNYL-2'-DEOXYURIDINE-5'-TRIPHOSPHATE LITHIUM SALT
- NAPHTHOFLUORESCEIN-5-DUTP
- CHROMATIDE(TM) BODIPY(R) 650/665-14-DUTP
- CHROMATIDE(TM) TEXAS RED(R)-5-UTP
- COUMARIN-5-DUTP
- LISSAMINE-5-DUTP
- COUMARIN-5-UTP
- TETRAMETHYLRHODAMINE-6-DDUTP