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3,4,5,6-TETRACHLOROFLUORESCEIN

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3,4,5,6-TETRACHLOROFLUORESCEIN Basic information

Product Name:
3,4,5,6-TETRACHLOROFLUORESCEIN
Synonyms:
  • 3,6-Dihydroxy-4',5',6',7'-tetrachlorospiro[9H-xanthene-9,1'(3'H)-isobenzofuran]-3'-one
  • 3',6'-Dihydroxy-4,5,6,7-tetrachlorospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one
  • Einecs 228-413-3
  • Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 4,5,6,7-tetrachloro-3',6'-dihydroxy-
  • TETRACHLOROFLUORESCEIN
  • 4,5,6,7-TETRACHLOROFLUORESCEIN
  • 9’-(9H)xanthen)-3-one,4,5,6,7-tetrachloro-3’,6’-dihydroxy-Spiro(isobenzofuran-1(3H)
  • 4,5,6,7-tetrachloro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one
CAS:
6262-21-1
MF:
C20H8Cl4O5
MW:
470.09
EINECS:
228-413-3
Product Categories:
  • Stains and Dyes
  • Stains&Dyes, A to
  • T-U-V
Mol File:
6262-21-1.mol
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3,4,5,6-TETRACHLOROFLUORESCEIN Chemical Properties

Melting point:
304℃
Boiling point:
752.3±60.0 °C(Predicted)
Density 
1.87±0.1 g/cm3(Predicted)
form 
powder to crystal
pka
9.18±0.20(Predicted)
color 
Light yellow to Amber to Dark green
CAS DataBase Reference
6262-21-1(CAS DataBase Reference)
EPA Substance Registry System
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 4,5,6,7-tetrachloro-3',6'-dihydroxy- (6262-21-1)
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Safety Information

WGK Germany 
3
TSCA 
Yes
HS Code 
29329990

MSDS

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3,4,5,6-TETRACHLOROFLUORESCEIN Usage And Synthesis

Description

3,4,5,6-Tetrachlorofluorescein is a reactive and monomeric dye that is used in analytical chemistry as a diagnostic agent. It reacts with proteins or peptides to form fluorescent complexes. 3,4,5,6-Tetrachlorofluorescein has been shown to bind to erythrocyte membrane proteins and is excreted in urine. This dye can be used for the detection of protease activity in human serum. 3,4,5,6-Tetrachlorofluorescein has also been used as an innovative pharmaceutical preparation for the treatment of chronic venous ulcers.

Chemical Properties

Appearance: Light yellow to Brown powder to crystal, Absorbance(E1%1cm): min. 3.50(Pyridine, 466.0 to 470.0 nm), Maximum Absorption Wavelength: 468(Pyridine) nm.

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