Fluorescein Sodium Chemical Properties

Melting point:

320 °C

Density 

0.579[at 20℃]

vapor pressure 

2.133hPa

storage temp. 

Store at +5°C to +30°C.

solubility 

H2O: soluble1mg/mL

form 

Powder

pka

2.2, 4.4, 6.7(at 25℃)

Colour Index 

45350

color 

Rust-orange

PH Range

Pink uorescence (4.0) to green uorescence (6.0)

PH

8.3 (10g/l, H2O, 20℃)

Odor

Odorless

Water Solubility 

500 g/L (20 ºC)

λmax

491nm, 493.5nm, 490nm

Merck 

14,4159

BRN 

3833041

Stability:

Stable.

Major Application

Nanolithography, nanoparticles, thin films, fuel cells, inks, laser, colored bubbles, cleansing products, identifying leaks in coolant, suntanning agent, hair dyes, cosmetics, dermatology, dental material, diagnosis of diabetic retinopathy, antitumor agent

InChIKey

NJDNXYGOVLYJHP-UHFFFAOYSA-L

LogP

0.34

CAS DataBase Reference

518-47-8(CAS DataBase Reference)

EPA Substance Registry System

Fluorescein, disodium salt (518-47-8)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36-36/37/38

Safety Statements 

22-24/25-37/39-26-36/37/39-27

WGK Germany 

1

RTECS 

LM5425000

F 

3

TSCA 

Yes

HS Code 

32041200

Hazardous Substances Data

518-47-8(Hazardous Substances Data)

Toxicity

LD50 in mice, rats (mg/kg): 4738, 6721 orally (Yankel, Loux)

MSDS

• Language:English Provider:C.I. 45350
• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

Fluorescein Sodium Usage And Synthesis

Chemical Properties

Orange-red powder; odorless. Hygroscopic, soluble in water; sparingly soluble in alcohol.

Uses

Fluorescein sodium salt is a sodium salt of fluorescein. It can also be used in technical or engineered material use in-situ photochemical fabrication of transition metal-?promoted amorphous molybdenum sulfide catalysts for enhanced photosensitized hydrogen evolution. Dyes and metabolites, Environmental Testing

Uses

In examining subterranean waters. Serves to ascertain source of springs, connections between streams and sea, determining approx vol of water delivered by a spring, detecting source of contamination of drinking water, infiltration of soil with waste waters of factories. In externally applied drugs and cosmetics. Analytical reagent (protein label). Clinical reagent (immuno-histological stain, immuno-fluorescent label).

General Description

Fluorescein sodium is an orange-red to dark red powder. Water-soluble form of fluorescein. Odorless and almost tasteless. pH (5% solution) 7.8. May be sensitive to prolonged exposure to light and to heat.

Air & Water Reactions

Hygroscopic. Water soluble.

Reactivity Profile

Fluorescein disodium salt is incompatible with strong oxidizers. Also incompatible with acids, acid salts and salts of heavy metals .

Fire Hazard

Flash point data for Fluorescein disodium salt are not available; however, Fluorescein disodium salt is probably combustible.

Safety Profile

Moderately toxic by intraperitoneal route. Mildly toxic by ingestion. Human systemic effects by intravenous route: arrhythmias, eye hemorrhage, nausea or vomiting. Experimental reproductive effects. Questionable carcinogen with experimental tumorigenic data. Mutation data reported. When heated to decomposition it emits toxic fumes of Na2O.

Veterinary Drugs and Treatments

Fluorescein sodium is a yellow water-soluble dye that fluoresces under a Wood’s Lamp, but is plainly visible after binding to corneal stroma through an ophthalmic examination light source. It is used most commonly to delineate full thickness loss of corneal epithelium indicating the presence of a corneal ulceration. In this instance it will stain the corneal stroma. The epithelium is not stained because its outer lipid cell membrane repels the stain. Descemet’s membrane will not stain with fluorescein stain and this is used to indicate descemetocele formation, an ocular emergency.
Fluorescein stain is applied to the precorneal tear film in dogs and cats and the break-up of this stain with time, as observed through a slit lamp biomicroscope using a cobalt blue light source, is used to determine the tear film break-up time (normal 19 seconds), an indicator of tear film quality.
Fluorescein stain is applied to the tear film of dogs to determine patency of the nasolacrimal outflow system. The normal wait time is 2 – 5 minutes in dogs and up to 10 minutes in cats. A positive test indicates patency of the system. A negative test is not indicative of disease as the test is negative in a large percentage of normal animals. Fluorescein stain, then, can be added to irrigating solution to flush the nasolacrimal system, making detecting the irrigation solution at the nose more obvious during flushing of the system.

Properties and Applications

brilliant yellow. Soluble in water and ethanol (with strong green fluorescent), water-soluble good. In strong sulfuric acid with weak for fluorescence yellow, it will be diluted with yellow precipitation. Its water solution and sodium hydroxide with dark green fluorescence for brunet solution. Mainly used for sea of target that fluorescent, facilitate aerial reconnaissance search. And can use significantly for the exploration of fluorescent groundwater. Can also be used for medicine and cosmetics shading, rarely used for silk printing and dyeing things.

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