Fluorescein isothiocyanate Chemical Properties

Melting point:

>360 °C(lit.)

storage temp. 

-20°C

solubility 

DMSO (Slightly), Methanol (Slightly)

form 

powder

color 

Brown to Dark Brown

Merck 

14,4089

BRN 

1407295

Stability:

Moisture Sensitive

InChIKey

WBSWSUYBEIMVMI-UHFFFAOYSA-N

CAS DataBase Reference

27072-45-3(CAS DataBase Reference)

EPA Substance Registry System

Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5(or 6)-isothiocyanato- (27072-45-3)

Safety Information

Hazard Codes 

Xn

Risk Statements 

42/43-36/37/38-20/21/22-42

Safety Statements 

22-36-26-24/25-45-36/37

WGK Germany 

3

F 

10

HS Code 

29321900

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

Fluorescein isothiocyanate Usage And Synthesis

Description

Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. The isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)
Excitation source: 488 nm spectral line, argon-ion laser
Excitation wavelength: 494 nm
Emission wavelength: 518 nm
Extinction coefficient: > 70,000 M-1cm-1
Solubility: Soluble in aqueous buffers at pH > 6
Reactive groups: Isothiocyanate, reacts with primary amines at pH 7.0 to 9.0

Chemical Properties

Dark yellow powder, soluble in water and decomposed in water. It is easily soluble in alkaline aqueous solution, and the strong green fluorescence disappears and precipitates. After adding alkali, it dissolves and shows fluorescence. Insoluble in acetone, ether and petroleum ether. Hygroscopic.

Uses

Fluorescein Isothiocyanate is a fluorescent reagent with incompletely defined position of the NCS. Fluorescein Isothiocyanate is used in multifunctional uniform nanoparticles composed of a magnetite nanocrystal core and a mesoporous silica shell for magnetic resonance, fluorescence imaging. and drug delivery. Dyes and metabolites.

Uses

For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography.
Label antibodies for use as immunofluorescent probes.
Label oligonucleotides for hybridization probes.
Detect proteins in gels and on Western blots.

Application

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.

Definition

ChEBI: Fluorescein 5-isothiocyanate is the 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

Preparation

By 4-nitrophthalic anhydride and resorcinol at 195-200 ℃ for 10h, cyclized into a mixture of 3'-nitrofluorescein and 4'-nitrofluorescein, and then acetylated with acetic anhydride , add saturated sodium hydroxide-ethanol solution for hydrolysis to obtain 4'-nitrofluorescein. Then, 4'-nitrofluorescein was dissolved in ammonia water, and Nickel Reishi and hydrazine hydrate were added to react at room temperature for 2 hours, and then reduced to 4'-aminofluorescein. The most elevated by acylation, elimination of fluorescein isothiocyanate.

Fluorescein isothiocyanate (27072-45-3)Related Product Information

• Sodium thiocyanate
• Ammonium thiocyanate
• Phenyl isothiocyanate
• Potassium thiocyanate
• Erythromycin thiocyanate
• Fluorescein
• Fluorescein isothiocyanate isomer I
• 3-S-Isothiuronium propyl sulfonate
• BENZYL ISOTHIOCYANATE
• Isocitric acid trisodium salt
• Isochlorogenic acid C
• Guanidine thiocyanate
• RHODAMINE B ISOTHIOCYANATE
• FLUORESCEIN ISOTHIOCYANATE-DEXTRAN
• 2-CHLOROETHYL ISOTHIOCYANATE
• Ethoxycarbonyl Isothiocyanate
• Isopropyl isothiocyanate
• ACETYL ISOTHIOCYANATE