Basic information Safety Supplier Related
ChemicalBook >  Product Catalog >  API >  Diagnostic agents >  Organ Function Test agent >  Fluorescein isothiocyanate

Fluorescein isothiocyanate

Basic information Safety Supplier Related

Fluorescein isothiocyanate Basic information

Product Name:
Fluorescein isothiocyanate
Synonyms:
  • FluoresceinIsothiocyanate,Isomers1&11
  • Spiroisobenzofuran-1(3H),9-9Hxanthen-3-one, 3,6-dihydroxy-5(or 6)-isothiocyanato-
  • Fluorescein 5(6)-isothiocyanate,FITC, Fluorescein isothiocyanate
  • Fluorescein5(6)-isot
  • 5(6)-FITC
  • Fluorescein 5(6)-isothiocyanat, mixture of isomers >90%
  • Fluorescein Isothiocyanate (Mixture of 5- and 6- isoMers)
  • Isothiocyanatofluorescein
CAS:
27072-45-3
MF:
C21H11NO5S
MW:
389.38
EINECS:
248-207-7
Product Categories:
  • FLUOROPHORES
Mol File:
27072-45-3.mol
More
Less

Fluorescein isothiocyanate Chemical Properties

Melting point:
>360 °C(lit.)
storage temp. 
-20°C
solubility 
DMSO (Slightly), Methanol (Slightly)
form 
powder
color 
Brown to Dark Brown
Merck 
14,4089
BRN 
1407295
Stability:
Moisture Sensitive
InChIKey
WBSWSUYBEIMVMI-UHFFFAOYSA-N
CAS DataBase Reference
27072-45-3(CAS DataBase Reference)
EPA Substance Registry System
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5(or 6)-isothiocyanato- (27072-45-3)
More
Less

Safety Information

Hazard Codes 
Xn
Risk Statements 
42/43-36/37/38-20/21/22-42
Safety Statements 
22-36-26-24/25-45-36/37
WGK Germany 
3
10
HS Code 
29321900

MSDS

More
Less

Fluorescein isothiocyanate Usage And Synthesis

Description

Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. The isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)
Excitation source: 488 nm spectral line, argon-ion laser
Excitation wavelength: 494 nm
Emission wavelength: 518 nm
Extinction coefficient: > 70,000 M-1cm-1
Solubility: Soluble in aqueous buffers at pH > 6
Reactive groups: Isothiocyanate, reacts with primary amines at pH 7.0 to 9.0

Chemical Properties

Dark yellow powder, soluble in water and decomposed in water. It is easily soluble in alkaline aqueous solution, and the strong green fluorescence disappears and precipitates. After adding alkali, it dissolves and shows fluorescence. Insoluble in acetone, ether and petroleum ether. Hygroscopic.

Uses

Fluorescein Isothiocyanate is a fluorescent reagent with incompletely defined position of the NCS. Fluorescein Isothiocyanate is used in multifunctional uniform nanoparticles composed of a magnetite nanocrystal core and a mesoporous silica shell for magnetic resonance, fluorescence imaging. and drug delivery. Dyes and metabolites.

Uses

For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography.
Label antibodies for use as immunofluorescent probes.
Label oligonucleotides for hybridization probes.
Detect proteins in gels and on Western blots.

Application

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.

Definition

ChEBI: Fluorescein 5-isothiocyanate is the 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

Preparation

By 4-nitrophthalic anhydride and resorcinol at 195-200 ℃ for 10h, cyclized into a mixture of 3'-nitrofluorescein and 4'-nitrofluorescein, and then acetylated with acetic anhydride , add saturated sodium hydroxide-ethanol solution for hydrolysis to obtain 4'-nitrofluorescein. Then, 4'-nitrofluorescein was dissolved in ammonia water, and Nickel Reishi and hydrazine hydrate were added to react at room temperature for 2 hours, and then reduced to 4'-aminofluorescein. The most elevated by acylation, elimination of fluorescein isothiocyanate.

Fluorescein isothiocyanate Preparation Products And Raw materials

Raw materials

Fluorescein isothiocyanate Supplier

Okeanos Tech Co,. Ltd. Gold
Tel
010-62651721 18610486005
Email
minghui.yu@okeanos.com.cn
Suzhouhongxiong Gold
Tel
18051745768 18051745768
Email
Suzhouhongxiong@163.com
Zhengzhou Jicheng Chemical Technology Co., Ltd Gold
Tel
15737124187; 15737124187
Email
1811685958@qq.com
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Email
jkinfo@jkchemical.com
INTATRADE GmbH
Tel
+49 3493/605464
Email
sales@intatrade.de