Fluorescein isothiocyanate
- Product Name
- Fluorescein isothiocyanate
- CAS No.
- 27072-45-3
- Chemical Name
- Fluorescein isothiocyanate
- Synonyms
- 5-FLUORESCEIN ISOTHIOCYANATE;FLUORESCEIN 5-ISOTHIOCYANATE;5(6)-Fluorescein isothiocyanate;5(6)-FITC;ocyanato-;FITC–Celite;FITC ISOMER;FITC, ISOMER I;FITC-CELITE(R);FITC 'ISOMER I'
- CBNumber
- CB3173163
- Molecular Formula
- C21H11NO5S
- Formula Weight
- 389.38
- MOL File
- 27072-45-3.mol
Fluorescein isothiocyanate Property
- Melting point:
- >360 °C(lit.)
- storage temp.
- -20°C
- solubility
- DMSO (Slightly), Methanol (Slightly)
- form
- powder
- color
- Brown to Dark Brown
- Merck
- 14,4089
- BRN
- 1407295
- Stability:
- Moisture Sensitive
- InChIKey
- WBSWSUYBEIMVMI-UHFFFAOYSA-N
- CAS DataBase Reference
- 27072-45-3(CAS DataBase Reference)
- EPA Substance Registry System
- Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5(or 6)-isothiocyanato- (27072-45-3)
Safety
- Hazard Codes
- Xn
- Risk Statements
- 42/43-36/37/38-20/21/22-42
- Safety Statements
- 22-36-26-24/25-45-36/37
- WGK Germany
- 3
- F
- 10
- HS Code
- 29321900
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Danger
- Hazard statements
-
H315Causes skin irritation
H317May cause an allergic skin reaction
H319Causes serious eye irritation
H334May cause allergy or asthma symptoms or breathing difficulties if inhaled
H335May cause respiratory irritation
- Precautionary statements
-
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P270Do not eat, drink or smoke when using this product.
P271Use only outdoors or in a well-ventilated area.
P272Contaminated work clothing should not be allowed out of the workplace.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P284Wear respiratory protection.
P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.
P403+P233Store in a well-ventilated place. Keep container tightly closed.
P405Store locked up.
P501Dispose of contents/container to..…
N-Bromosuccinimide Price
- Product number
- 46950
- Product name
- Fluorescein 5(6)-isothiocyanate
- Purity
- BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
- Packaging
- 50MG
- Price
- $42.9
- Updated
- 2024/03/01
- Product number
- 46950
- Product name
- Fluorescein 5(6)-isothiocyanate
- Purity
- BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
- Packaging
- 250MG
- Price
- $152
- Updated
- 2024/03/01
- Product number
- 46950
- Product name
- Fluorescein 5(6)-isothiocyanate
- Purity
- BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
- Packaging
- 1G
- Price
- $426
- Updated
- 2024/03/01
- Product number
- F0784
- Product name
- Fluorescein Isothiocyanate (mixture of 5- and 6- isomers)
- Purity
- >97.0%(HPLC)(T)
- Packaging
- 1g
- Price
- $1250
- Updated
- 2024/03/01
- Product number
- F0784
- Product name
- Fluorescein Isothiocyanate (mixture of 5- and 6- isomers)
- Purity
- >97.0%(HPLC)(T)
- Packaging
- 100mg
- Price
- $141
- Updated
- 2024/03/01
Fluorescein isothiocyanate Chemical Properties,Usage,Production
Description
Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. The isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)
Excitation source: 488 nm spectral line, argon-ion laser
Excitation wavelength: 494 nm
Emission wavelength: 518 nm
Extinction coefficient: > 70,000 M-1cm-1
Solubility: Soluble in aqueous buffers at pH > 6
Reactive groups: Isothiocyanate, reacts with primary amines at pH 7.0 to 9.0
Chemical Properties
Dark yellow powder, soluble in water and decomposed in water. It is easily soluble in alkaline aqueous solution, and the strong green fluorescence disappears and precipitates. After adding alkali, it dissolves and shows fluorescence. Insoluble in acetone, ether and petroleum ether. Hygroscopic.
Uses
Fluorescein Isothiocyanate is a fluorescent reagent with incompletely defined position of the NCS. Fluorescein Isothiocyanate is used in multifunctional uniform nanoparticles composed of a magnetite nanocrystal core and a mesoporous silica shell for magnetic resonance, fluorescence imaging. and drug delivery. Dyes and metabolites.
Uses
For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography.
Label antibodies for use as immunofluorescent probes.
Label oligonucleotides for hybridization probes.
Detect proteins in gels and on Western blots.
Application
Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.
Definition
ChEBI: Fluorescein 5-isothiocyanate is the 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
Preparation
By 4-nitrophthalic anhydride and resorcinol at 195-200 ℃ for 10h, cyclized into a mixture of 3'-nitrofluorescein and 4'-nitrofluorescein, and then acetylated with acetic anhydride , add saturated sodium hydroxide-ethanol solution for hydrolysis to obtain 4'-nitrofluorescein. Then, 4'-nitrofluorescein was dissolved in ammonia water, and Nickel Reishi and hydrazine hydrate were added to react at room temperature for 2 hours, and then reduced to 4'-aminofluorescein. The most elevated by acylation, elimination of fluorescein isothiocyanate.
Fluorescein isothiocyanate Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from Fluorescein isothiocyanate manufacturers
- Product
- Fluorescein isothiocyanate 27072-45-3
- Price
- US $100.00/bag
- Min. Order
- 1bag
- Purity
- 99
- Supply Ability
- 5000
- Release date
- 2023-09-07
- Product
- Fluorescein isothiocyanate 27072-45-3
- Price
- US $0.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 50000KG/month
- Release date
- 2023-07-28
- Product
- Fluorescein isothiocyanate 27072-45-3
- Price
- US $0.00-0.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 20 mt
- Release date
- 2023-03-24