ChemicalBook > CAS DataBase List > Fluorescein isothiocyanate isomer I

Fluorescein isothiocyanate isomer I

Product Name
Fluorescein isothiocyanate isomer I
CAS No.
3326-32-7
Chemical Name
Fluorescein isothiocyanate isomer I
Synonyms
to-;FITC;FITC;FITC;5-FITC;FITC-I;FITC-I)F;FITC ISOMER;PLAUR)FITC-CELITE(R)
CBNumber
CB0173162
Molecular Formula
C21H11NO5S
Formula Weight
389.38
MOL File
3326-32-7.mol
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Fluorescein isothiocyanate isomer I Property

Melting point:
>360 °C(lit.)
Boiling point:
708.6±60.0 °C(Predicted)
Density 
1.3423 (rough estimate)
refractive index 
1.5060 (estimate)
storage temp. 
2-8°C
solubility 
Slightly soluble in DMSO, ethanol, methanol DMF and acetone
pka
2.2, 4.4, 6.7(at 25℃)
form 
powder
color 
White
PH Range
Weak green uorescence (6.0) to strong green uorescence (7.2)
Water Solubility 
practically insoluble
λmax
490nm
Sensitive 
Moisture Sensitive
BRN 
1407295
Stability:
Stability Stable, but moisture-sensitive. Incompatible with strong oxidizing agents, strong bases, amines, acids.
Major Application
Magnetic nanowires, immunoassays, analyzing proteins, identifying chromosomes, Salmonella mosomes, diagnosis of cancer, kidney diseases, detecting pathogens, genes, Salmonella cells, toxoplasmosis, enzyme-mediated nucleic acid cleavage, surface molecules on colorectal cancers, treating cancer, chronic lymphocytic leukemia
Biological Applications
Analyzing sugar chain; detecting iodide/iodate or total iodine; detecting/imaging thiols; detecting/quantifying biotin derivatives;16 diagnosing classical Hodgkin lymphoma (CHL) in lymph nodes; cellulose nanocrystals for bioimaging applications; treating neuroinflammation; treating/diagnosing cancer; PEO-PCL-PEO triblock copolymers for topical delivery; polymersomes in biomedical or cosmetic applications;apoptosis assay; as a substrate for measuring heparanase activity, human immunodeficiency virus (HIV-1) proteinase activity, kinase activity, phosphatase activity, β-lactamase activity, transglutaminase (TG2) activity; implantable drug-delivery devicess
InChIKey
MHMNJMPURVTYEJ-UHFFFAOYSA-N
CAS DataBase Reference
3326-32-7(CAS DataBase Reference)
EPA Substance Registry System
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato- (3326-32-7)
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Safety

Hazard Codes 
Xn,Xi
Risk Statements 
42/43-36/37/38-22
Safety Statements 
22-45-36/37/39-26-36/37-36
WGK Germany 
3
10
TSCA 
Yes
HS Code 
29329990
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H315Causes skin irritation

H317May cause an allergic skin reaction

H319Causes serious eye irritation

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

H335May cause respiratory irritation

Precautionary statements

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P262Do not get in eyes, on skin, or on clothing.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P271Use only outdoors or in a well-ventilated area.

P272Contaminated work clothing should not be allowed out of the workplace.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P284Wear respiratory protection.

P309IF exposed or if you feel unwell:

P310Immediately call a POISON CENTER or doctor/physician.

P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.

P405Store locked up.

P403+P233Store in a well-ventilated place. Keep container tightly closed.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
F3651
Product name
Fluorescein 5(6)-isothiocyanate
Purity
≥90% (HPLC)
Packaging
100 mg
Price
$87.6
Updated
2021/03/22
Sigma-Aldrich
Product number
34321-M
Product name
FITC, Isomer I
Packaging
1 g
Price
$240.5
Updated
2021/03/22
Sigma-Aldrich
Product number
34321-M
Product name
FITC, Isomer I
Packaging
1gm-m
Price
$260
Updated
2020/08/18
Sigma-Aldrich
Product number
1.24546
Product name
Fluorescein-5-isothiocyanate (FITC)
Purity
for biochemistry and for fluorescence microscopy
Packaging
250 mg
Price
$345.02
Updated
2021/03/22
Sigma-Aldrich
Product number
46951
Product name
Fluorescein isothiocyanate isomer I
Purity
≥97.5% (HPLC)
Packaging
250mg-f
Price
$483
Updated
2020/08/18
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Fluorescein isothiocyanate isomer I Chemical Properties,Usage,Production

Chemical Properties

Orange-yellow cryst.

Uses

Fluorescein -5-isothiocyanate is a kind of fluorescent marker for biochemical applications. Reacts under mild conditions with primary amines and it is also used in modification of actin at lys-61, labelling of FAB and the modification of thiol groups.

Uses

Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens

Definition

ChEBI: The 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

Purification Methods

It is made from the pure 5-amino isomer. Purify it by dissolving it in boiling Me2CO, filtering and adding pet ether (b 60-70o) until it becomes turbid. If an oil separates, then decant it and add more pet ether to the supernatant and cool. Orange-yellow crystals separate, collect and dry them in vacuo. It should give one spot on TLC (silica gel) in EtOAc/pyridine/AcOH (50:1:1) and in Me2NCHO/CHCl3/28% NH4OH (10:5:4). IR (Me2SO): 2110 (NCS) and 1760 (C=O) cm-1. The max 1HNMR spectra in Me2CO-d6 of the 5-and 6-isomers are distinctly different for the protons in the *benzene ring; the UV in phosphate buffer pH 8.0 shows a at ~490nm. [Sinsheimer et al. Anal Biochem 57 2271974, McKinney et al. Anal Biochem 7 74 1964, Beilstein 19 III/IV 4337.]

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Fluorescein isothiocyanate isomer I Suppliers

Okeanos Tech Co,. Ltd.
Tel
010-62651721-
Fax
01062651521-808
Email
info@okeanos.com.cn
Country
China
ProdList
476
Advantage
60
Nanjing UltraPure Biotech Co., Ltd.
Tel
025-82263158-
Fax
025-82263168
Email
sales@ultrapurebio.com
Country
China
ProdList
216
Advantage
58
Creative Peptides
Email
info@creative-peptides.com
Country
United States
ProdList
6070
Advantage
56
J & K SCIENTIFIC LTD.
Tel
010-82848833- ;010-82848833-
Fax
86-10-82849933
Email
jkinfo@jkchemical.com;market6@jkchemical.com
Country
China
ProdList
96500
Advantage
76
Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
21-61259100-
Fax
86-21-61259102
Email
sh@meryer.com
Country
China
ProdList
40264
Advantage
62
INTATRADE GmbH
Tel
+49 3493/605464
Fax
+49 3493/605470
Email
sales@intatrade.de
Country
Germany
ProdList
3579
Advantage
66
3B Pharmachem (Wuhan) International Co.,Ltd.
Tel
821-50328103-801
Fax
86-21-50328109
Email
3bsc@sina.com
Country
China
ProdList
15877
Advantage
69
Alfa Aesar
Tel
400-610-6006; 021-67582000
Fax
021-67582001/03/05
Email
saleschina@alfa-asia.com
Country
China
ProdList
30159
Advantage
84
TCI (Shanghai) Development Co., Ltd.
Tel
021-67121386 / 800-988-0390
Fax
021-67121385
Email
Sales-CN@TCIchemicals.com
Country
China
ProdList
24555
Advantage
81
BeiJing Hwrk Chemicals Limted
Tel
0757-86329057-
Fax
0757-86311057
Email
sales.gd@hwrkchemical.com
Country
China
ProdList
17295
Advantage
55
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View Lastest Price from Fluorescein isothiocyanate isomer I manufacturers

Zhuozhou Wenxi import and Export Co., Ltd
Product
Fluorescein 5-Isothiocyanate (isomer I) 3326-32-7
Price
US $15.00-10.00/KG
Min. Order
1KG
Purity
99%+ HPLC
Supply Ability
Monthly supply of 1 ton
Release date
2021-06-27
career henan chemical co
Product
Fluorescein isothiocyanate isomer I 3326-32-7
Price
US $1.00/kg
Min. Order
1kg
Purity
98%
Supply Ability
100KG
Release date
2018-12-21

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