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Fluorescein isothiocyanate isomer I

Product Name
Fluorescein isothiocyanate isomer I
CAS No.
3326-32-7
Chemical Name
Fluorescein isothiocyanate isomer I
Synonyms
FITC;FITC;FITC;5-FITC;Fluorescein isothiocyanate isomer;5-FLUORESCEIN ISOTHIOCYANATE;Fluorescein isothiocyanate isomer I, tech grade;FITC-I;FLUORESCEIN 5-ISOTHIOCYANATE;Fluorescein isothiocyanate I
CBNumber
CB0173162
Molecular Formula
C21H11NO5S
Formula Weight
389.38
MOL File
3326-32-7.mol
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Fluorescein isothiocyanate isomer I Property

Melting point:
>360 °C(lit.)
Boiling point:
708.6±60.0 °C(Predicted)
Density 
1.3423 (rough estimate)
refractive index 
1.5060 (estimate)
storage temp. 
2-8°C
solubility 
Slightly soluble in DMSO, ethanol, methanol DMF and acetone
pka
2.2, 4.4, 6.7(at 25℃)
form 
powder
color 
White
PH Range
Weak green uorescence (6.0) to strong green uorescence (7.2)
Water Solubility 
practically insoluble
Sensitive 
Moisture Sensitive
λmax
490nm
BRN 
1407295
Stability:
Stability Stable, but moisture-sensitive. Incompatible with strong oxidizing agents, strong bases, amines, acids.
Major Application
Magnetic nanowires, immunoassays, analyzing proteins, identifying chromosomes, Salmonella mosomes, diagnosis of cancer, kidney diseases, detecting pathogens, genes, Salmonella cells, toxoplasmosis, enzyme-mediated nucleic acid cleavage, surface molecules on colorectal cancers, treating cancer, chronic lymphocytic leukemia
Biological Applications
Analyzing sugar chain; detecting iodide/iodate or total iodine; detecting/imaging thiols; detecting/quantifying biotin derivatives;16 diagnosing classical Hodgkin lymphoma (CHL) in lymph nodes; cellulose nanocrystals for bioimaging applications; treating neuroinflammation; treating/diagnosing cancer; PEO-PCL-PEO triblock copolymers for topical delivery; polymersomes in biomedical or cosmetic applications;apoptosis assay; as a substrate for measuring heparanase activity, human immunodeficiency virus (HIV-1) proteinase activity, kinase activity, phosphatase activity, β-lactamase activity, transglutaminase (TG2) activity; implantable drug-delivery devicess
InChIKey
MHMNJMPURVTYEJ-UHFFFAOYSA-N
CAS DataBase Reference
3326-32-7(CAS DataBase Reference)
EPA Substance Registry System
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato- (3326-32-7)
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Safety

Hazard Codes 
Xn,Xi
Risk Statements 
42/43-36/37/38-22
Safety Statements 
22-45-36/37/39-26-36/37-36
WGK Germany 
3
10
TSCA 
Yes
HS Code 
29329990
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H317May cause an allergic skin reaction

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P272Contaminated work clothing should not be allowed out of the workplace.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P284Wear respiratory protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P333+P313IF SKIN irritation or rash occurs: Get medical advice/attention.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
46951
Product name
Fluorescein isothiocyanate isomer I
Purity
≥97.5% (HPLC)
Packaging
250mg
Price
$620
Updated
2024/03/01
TCI Chemical
Product number
F0026
Product name
Fluorescein 5-Isothiocyanate (isomer I)
Purity
>97.0%(HPLC)(T)
Packaging
1g
Price
$388
Updated
2024/03/01
TCI Chemical
Product number
F0026
Product name
Fluorescein 5-Isothiocyanate (isomer I)
Purity
>97.0%(HPLC)(T)
Packaging
100mg
Price
$61
Updated
2024/03/01
Alfa Aesar
Product number
L09319
Product name
Fluorescein isothiocyanate, isomer 1, 95%
Packaging
50mg
Price
$38.8
Updated
2024/03/01
Alfa Aesar
Product number
L09319
Product name
Fluorescein isothiocyanate, isomer 1, 95%
Packaging
1g
Price
$306
Updated
2024/03/01
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Fluorescein isothiocyanate isomer I Chemical Properties,Usage,Production

Description

Fluorescein 5-isothiocyanate (5-FITC) is an isomer of fluorescein isothiocyanate (FITC), which is commonly used as a mixture of the 5- and 6-isothiocyanate isomers. It reacts with amine and thiol groups to form conjugates with proteins, lipids, and other molecules for detection by a variety of fluorescent-based applications. 5-FITC displays excitation/emission maxima of 494/519 nm, respectively.

Chemical Properties

Fluorescein isothiocyanate(3326-32-7) is an orange-yellow crystal that is hygroscopic. It can be combined with various antibody proteins, and the combined antibody does not lose its specificity for binding to a certain antigen, and still has strong green fluorescence in alkaline solution, precipitates out after adding acid, and the fluorescence disappears. It is slightly soluble in acetone and ether and petroleum ether.

Uses

Fluorescein isothiocyanate isomer I has been used for the staining of conidia. It is a kind of fluorescent marker for biochemical applications. Reacts under mild conditions with primary amines and it is also used in modification of actin at lys-61, labelling of FAB and the modification of thiol groups.

Application

Fluorescein isothiocyanate is used as fluorescent labeling reagent for proteins and for rapid identification of pathogens in fluorescent antibody technique. It is also used as a reagent for microsequencing of proteins and peptides (HPLC). On celite it is used for the labeling of fibrinogen.

Definition

ChEBI: Fluorescein 5-isothiocyanate is the 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

General Description

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.

storage

Store at -20°C

Purification Methods

It is made from the pure 5-amino isomer. Purify it by dissolving it in boiling Me2CO, filtering and adding pet ether (b 60-70o) until it becomes turbid. If an oil separates, then decant it and add more pet ether to the supernatant and cool. Orange-yellow crystals separate, collect and dry them in vacuo. It should give one spot on TLC (silica gel) in EtOAc/pyridine/AcOH (50:1:1) and in Me2NCHO/CHCl3/28% NH4OH (10:5:4). IR (Me2SO): 2110 (NCS) and 1760 (C=O) cm-1. The max 1HNMR spectra in Me2CO-d6 of the 5-and 6-isomers are distinctly different for the protons in the *benzene ring; the UV in phosphate buffer pH 8.0 shows a at ~490nm. [Sinsheimer et al. Anal Biochem 57 2271974, McKinney et al. Anal Biochem 7 74 1964, Beilstein 19 III/IV 4337.]

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Fluorescein isothiocyanate isomer I Suppliers

Suzhouhongxiong
Tel
18051745768 18051745768
Email
Suzhouhongxiong@163.com
Country
China
ProdList
100
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58
Okeanos Tech Co,. Ltd.
Tel
010-62651721 18610486005
Fax
01062651521-808
Email
minghui.yu@okeanos.com.cn
Country
China
ProdList
493
Advantage
60
Nanjing UltraPure Biotech Co., Ltd.
Tel
025-82263158 13851993283
Fax
025-82263168
Email
2842979086@qq.com
Country
China
ProdList
241
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58
Aikon International Limited
Tel
025-58859352 18068836627
Fax
02557626880
Email
sales01@aikonchem.com
Country
China
ProdList
15085
Advantage
58
Shanghai CanSpecsci Instrument Co., Ltd.
Tel
400-6087598 15021221957
Fax
4006087598-8012
Email
order@canspecsci.com
Country
China
ProdList
2079
Advantage
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Changzhou Ansciep Chemical Co., Ltd.
Tel
0519-86305871 2719466718
Email
tina@ansciepchem.com
Country
China
ProdList
1553
Advantage
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J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
96815
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Meryer (Shanghai) Chemical Technology Co., Ltd.
Tel
4006608290; 18621169109
Fax
86-21-61259102
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market03@meryer.com
Country
China
ProdList
40241
Advantage
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INTATRADE GmbH
Tel
+49 3493/605464
Fax
+49 3493/605470
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sales@intatrade.de
Country
Germany
ProdList
3576
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3B Pharmachem (Wuhan) International Co.,Ltd.
Tel
821-50328103-801 18930552037
Fax
86-21-50328109
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3bsc@sina.com
Country
China
ProdList
15848
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View Lastest Price from Fluorescein isothiocyanate isomer I manufacturers

Hebei Duling International Trade Co. LTD
Product
Fluorescein isothiocyanate isomer I 3326-32-7
Price
US $33.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
5000 tons
Release date
2023-02-02
Hebei Yanxi Chemical Co., Ltd.
Product
Fluorescein isothiocyanate isomer I 3326-32-7
Price
US $30.00-9.00/kg
Min. Order
1kg
Purity
0.99
Supply Ability
20 tons
Release date
2023-09-25
Hebei Mojin Biotechnology Co., Ltd
Product
Fluorescein isothiocyanate isomer I 3326-32-7
Price
US $0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
50000KG/month
Release date
2023-11-13

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