Fluorescein isothiocyanate

ChemicalBook > CAS DataBase List > Fluorescein isothiocyanate

Fluorescein isothiocyanate

Product Name: Fluorescein isothiocyanate
CAS No.: 27072-45-3
Chemical Name: Fluorescein isothiocyanate
Synonyms: 5-FLUORESCEIN ISOTHIOCYANATE;FLUORESCEIN 5-ISOTHIOCYANATE;5(6)-Fluorescein isothiocyanate;5(6)-FITC;ocyanato-;FITC–Celite;FITC ISOMER;FITC, ISOMER I;FITC-CELITE(R);FITC 'ISOMER I'
CBNumber: CB3173163
Molecular Formula: C21H11NO5S
Formula Weight: 389.38
MOL File: 27072-45-3.mol

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Fluorescein isothiocyanate Property

Melting point:: >360 °C(lit.)
storage temp.: -20°C
solubility: DMSO (Slightly), Methanol (Slightly)
form: powder
color: Brown to Dark Brown
Merck: 14,4089
BRN: 1407295
Stability:: Moisture Sensitive
InChIKey: WBSWSUYBEIMVMI-UHFFFAOYSA-N
CAS DataBase Reference: 27072-45-3(CAS DataBase Reference)
EPA Substance Registry System: Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5(or 6)-isothiocyanato- (27072-45-3)

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Safety

Hazard Codes: Xn
Risk Statements: 42/43-36/37/38-20/21/22-42
Safety Statements: 22-36-26-24/25-45-36/37
WGK Germany: 3
F: 10
HS Code: 29321900

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H315Causes skin irritation

H317May cause an allergic skin reaction

H319Causes serious eye irritation

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P271Use only outdoors or in a well-ventilated area.

P272Contaminated work clothing should not be allowed out of the workplace.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P284Wear respiratory protection.

P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.

P403+P233Store in a well-ventilated place. Keep container tightly closed.

P405Store locked up.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 46950
Product name: Fluorescein 5(6)-isothiocyanate
Purity: BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
Packaging: 50MG
Price: $42.9
Updated: 2024/03/01

Sigma-Aldrich

Product number: 46950
Product name: Fluorescein 5(6)-isothiocyanate
Purity: BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
Packaging: 250MG
Price: $152
Updated: 2024/03/01

Sigma-Aldrich

Product number: 46950
Product name: Fluorescein 5(6)-isothiocyanate
Purity: BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
Packaging: 1G
Price: $426
Updated: 2024/03/01

TCI Chemical

Product number: F0784
Product name: Fluorescein Isothiocyanate (mixture of 5- and 6- isomers)
Purity: >97.0%(HPLC)(T)
Packaging: 1g
Price: $1250
Updated: 2024/03/01

TCI Chemical

Product number: F0784
Product name: Fluorescein Isothiocyanate (mixture of 5- and 6- isomers)
Purity: >97.0%(HPLC)(T)
Packaging: 100mg
Price: $141
Updated: 2024/03/01

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Fluorescein isothiocyanate Chemical Properties,Usage,Production

Description

Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. The isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)
Excitation source: 488 nm spectral line, argon-ion laser
Excitation wavelength: 494 nm
Emission wavelength: 518 nm
Extinction coefficient: > 70,000 M-1cm-1
Solubility: Soluble in aqueous buffers at pH > 6
Reactive groups: Isothiocyanate, reacts with primary amines at pH 7.0 to 9.0

Chemical Properties

Dark yellow powder, soluble in water and decomposed in water. It is easily soluble in alkaline aqueous solution, and the strong green fluorescence disappears and precipitates. After adding alkali, it dissolves and shows fluorescence. Insoluble in acetone, ether and petroleum ether. Hygroscopic.

Uses

Fluorescein Isothiocyanate is a fluorescent reagent with incompletely defined position of the NCS. Fluorescein Isothiocyanate is used in multifunctional uniform nanoparticles composed of a magnetite nanocrystal core and a mesoporous silica shell for magnetic resonance, fluorescence imaging. and drug delivery. Dyes and metabolites.

Uses

For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography.
Label antibodies for use as immunofluorescent probes.
Label oligonucleotides for hybridization probes.
Detect proteins in gels and on Western blots.

Application

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.

Definition

ChEBI: Fluorescein 5-isothiocyanate is the 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

Preparation

By 4-nitrophthalic anhydride and resorcinol at 195-200 ℃ for 10h, cyclized into a mixture of 3'-nitrofluorescein and 4'-nitrofluorescein, and then acetylated with acetic anhydride , add saturated sodium hydroxide-ethanol solution for hydrolysis to obtain 4'-nitrofluorescein. Then, 4'-nitrofluorescein was dissolved in ammonia water, and Nickel Reishi and hydrazine hydrate were added to react at room temperature for 2 hours, and then reduced to 4'-aminofluorescein. The most elevated by acylation, elimination of fluorescein isothiocyanate.

Fluorescein isothiocyanate Preparation Products And Raw materials

Raw materials

5-Aminofluorescein Sodium hydroxide-ethanol solution Resorcinol 4-Nitrophthalic anhydride Fluorescein 4-Nitrofluorescein

Preparation Products

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View Lastest Price from Fluorescein isothiocyanate manufacturers

Hebei Fengqiang Trading Co., LTD

Product: Fluorescein isothiocyanate 27072-45-3
Price: US $100.00/bag
Min. Order: 1bag
Purity: 99
Supply Ability: 5000
Release date: 2023-09-07

Hebei Mojin Biotechnology Co., Ltd

Product: Fluorescein isothiocyanate 27072-45-3
Price: US $0.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 50000KG/month
Release date: 2023-07-28

Hebei Guanlang Biotechnology Co., Ltd.

Product: Fluorescein isothiocyanate 27072-45-3
Price: US $0.00-0.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 20 mt
Release date: 2023-03-24

27072-45-3, Fluorescein isothiocyanate Related Search:

Sodium thiocyanate Ammonium thiocyanate Phenyl isothiocyanate Potassium thiocyanate Erythromycin thiocyanate Fluorescein Fluorescein isothiocyanate isomer I 3-S-Isothiuronium propyl sulfonate BENZYL ISOTHIOCYANATE Isocitric acid trisodium salt Isochlorogenic acid C Guanidine thiocyanate RHODAMINE B ISOTHIOCYANATE FLUORESCEIN ISOTHIOCYANATE-DEXTRAN 2-CHLOROETHYL ISOTHIOCYANATE Ethoxycarbonyl Isothiocyanate Isopropyl isothiocyanate ACETYL ISOTHIOCYANATE

FluoresceinIsothiocyanate,Isomers1&11

Spiroisobenzofuran-1(3H),9-9Hxanthen-3-one, 3,6-dihydroxy-5(or 6)-isothiocyanato-

Fluorescein 5(6)-isothiocyanate,FITC, Fluorescein isothiocyanate

Fluorescein5(6)-isot

5(6)-FITC

Fluorescein 5(6)-isothiocyanat, mixture of isomers >90%

Fluorescein Isothiocyanate (Mixture of 5- and 6- isoMers)

Isothiocyanatofluorescein

FITC, Fluorescein isothiocyanate

Fuorescein isothiocyanate

5-lsothiocyanato fluorescein

5(6)-Fluorescein isothiocyanate

Fluorescein isothiocyanate, for fluorescence, Mixture of 5- and 6- isoMers

FITC–Celite

Fluorescein isothiocyanate isomer I–Celite

Flouresceiniso-thiocyanate

ocyanato-

spiro[isobenzofuran-1(3h),9’-[9h]xanthen]-3-one,3’,6’-dihydroxy-5(or6)-isothi

5(6)-FITC (Fluorescein 5(6)-isothiocyanate)

3',6'-dihydroxy-5(or 6)-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one

FLUORESCEIN ISOTHIOCYANATE, FOR FLUORESC ENCE

3',6'-Dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-onecompoundwithisothiocyanatomethane(1:1)

Fluorescein 5(6)-isothiocyanate, 95%, for fluorescence, mixture of 5- and 6- isomers

SPECTRACOMP(TM) FITC PARTICLES

2-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-5-ISOTHIOCYANATO-BENZOIC ACID

3',6'-Dihydroxy-5(or 6)-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one

5-FLUORESCEIN ISOTHIOCYANATE

5-FLUORESCEIN ISOTHIOCYANATE, ISOMER I

5-FLUORESCEIN ISOTHIOCYANATEISOMER

FLUORESCEIN-5-ISOTHIOCYANATE ISOMER I

FLUORESCEIN 5-ISOTHIOCYANATE, ISOMER 1

FLUORESCEIN 5(6)-ISOTHIOCYANATE

FLUORESCEIN 5-ISOTHIOCYANATE

FLUORESCEIN ISOMER I ISOTHIOCYANATE

FLUORESCEIN ISOTHIOCYANATE ISOMER I-CELITE(R)

FLUORESCEIN ISOTHIOCYANATE, ISOMER 1

FLUORESCEIN ISOTHIOCYANATE

FITC ISOMER

FITC 'ISOMER I'

FITC, ISOMER I

FITC-CELITE(R)

3',6'-dihydroxy-6-isothiocyanato-1-spiro[isobenzofuran-3,9'-xanthene]one

FluoresceinIsothiocyanate(mixtureof5-and6-isomers)&gt

Fluorescein isothiocyanate USP/EP/BP

Fluorescein Isothiocyanate (>85%)

Fluorescein 5(6)-isothiocyanate, mixture of 2 components, ≥90% (HPLC)

27072-45-3

3326-94-8

2C21H11NO5S

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