ChemicalBook > CAS DataBase List > Fluorescein isothiocyanate

Fluorescein isothiocyanate

Product Name
Fluorescein isothiocyanate
CAS No.
27072-45-3
Chemical Name
Fluorescein isothiocyanate
Synonyms
5-FLUORESCEIN ISOTHIOCYANATE;FLUORESCEIN 5-ISOTHIOCYANATE;5(6)-Fluorescein isothiocyanate;5(6)-FITC;ocyanato-;FITC–Celite;FITC ISOMER;FITC, ISOMER I;FITC-CELITE(R);FITC 'ISOMER I'
CBNumber
CB3173163
Molecular Formula
C21H11NO5S
Formula Weight
389.38
MOL File
27072-45-3.mol
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Fluorescein isothiocyanate Property

Melting point:
>360 °C(lit.)
storage temp. 
-20°C
solubility 
DMSO (Slightly), Methanol (Slightly)
form 
powder
color 
Brown to Dark Brown
Merck 
14,4089
BRN 
1407295
Stability:
Moisture Sensitive
InChIKey
WBSWSUYBEIMVMI-UHFFFAOYSA-N
CAS DataBase Reference
27072-45-3(CAS DataBase Reference)
EPA Substance Registry System
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5(or 6)-isothiocyanato- (27072-45-3)
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Safety

Hazard Codes 
Xn
Risk Statements 
42/43-36/37/38-20/21/22-42
Safety Statements 
22-36-26-24/25-45-36/37
WGK Germany 
3
F 
10
HS Code 
29321900
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H315Causes skin irritation

H317May cause an allergic skin reaction

H319Causes serious eye irritation

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P271Use only outdoors or in a well-ventilated area.

P272Contaminated work clothing should not be allowed out of the workplace.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P284Wear respiratory protection.

P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.

P403+P233Store in a well-ventilated place. Keep container tightly closed.

P405Store locked up.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
46950
Product name
Fluorescein 5(6)-isothiocyanate
Purity
BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
Packaging
50MG
Price
$42.9
Updated
2024/03/01
Sigma-Aldrich
Product number
46950
Product name
Fluorescein 5(6)-isothiocyanate
Purity
BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
Packaging
250MG
Price
$152
Updated
2024/03/01
Sigma-Aldrich
Product number
46950
Product name
Fluorescein 5(6)-isothiocyanate
Purity
BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
Packaging
1G
Price
$426
Updated
2024/03/01
TCI Chemical
Product number
F0784
Product name
Fluorescein Isothiocyanate (mixture of 5- and 6- isomers)
Purity
>97.0%(HPLC)(T)
Packaging
1g
Price
$1250
Updated
2024/03/01
TCI Chemical
Product number
F0784
Product name
Fluorescein Isothiocyanate (mixture of 5- and 6- isomers)
Purity
>97.0%(HPLC)(T)
Packaging
100mg
Price
$141
Updated
2024/03/01
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Fluorescein isothiocyanate Chemical Properties,Usage,Production

Description

Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. The isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)
Excitation source: 488 nm spectral line, argon-ion laser
Excitation wavelength: 494 nm
Emission wavelength: 518 nm
Extinction coefficient: > 70,000 M-1cm-1
Solubility: Soluble in aqueous buffers at pH > 6
Reactive groups: Isothiocyanate, reacts with primary amines at pH 7.0 to 9.0

Chemical Properties

Dark yellow powder, soluble in water and decomposed in water. It is easily soluble in alkaline aqueous solution, and the strong green fluorescence disappears and precipitates. After adding alkali, it dissolves and shows fluorescence. Insoluble in acetone, ether and petroleum ether. Hygroscopic.

Uses

Fluorescein Isothiocyanate is a fluorescent reagent with incompletely defined position of the NCS. Fluorescein Isothiocyanate is used in multifunctional uniform nanoparticles composed of a magnetite nanocrystal core and a mesoporous silica shell for magnetic resonance, fluorescence imaging. and drug delivery. Dyes and metabolites.

Uses

For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography.
Label antibodies for use as immunofluorescent probes.
Label oligonucleotides for hybridization probes.
Detect proteins in gels and on Western blots.

Application

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.

Definition

ChEBI: Fluorescein 5-isothiocyanate is the 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

Preparation

By 4-nitrophthalic anhydride and resorcinol at 195-200 ℃ for 10h, cyclized into a mixture of 3'-nitrofluorescein and 4'-nitrofluorescein, and then acetylated with acetic anhydride , add saturated sodium hydroxide-ethanol solution for hydrolysis to obtain 4'-nitrofluorescein. Then, 4'-nitrofluorescein was dissolved in ammonia water, and Nickel Reishi and hydrazine hydrate were added to react at room temperature for 2 hours, and then reduced to 4'-aminofluorescein. The most elevated by acylation, elimination of fluorescein isothiocyanate.

Fluorescein isothiocyanate Preparation Products And Raw materials

Raw materials

5-Aminofluorescein Sodium hydroxide-ethanol solution Resorcinol 4-Nitrophthalic anhydride Fluorescein 4-Nitrofluorescein

Preparation Products

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View Lastest Price from Fluorescein isothiocyanate manufacturers

Hebei Weibang Biotechnology Co., Ltd
Product
Fluorescein isothiocyanate 27072-45-3
Price
US $0.00-0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
20 mt
Release date
2024-11-19
Hebei Fengqiang Trading Co., LTD
Product
Fluorescein isothiocyanate 27072-45-3
Price
US $100.00/bag
Min. Order
1bag
Purity
99
Supply Ability
5000
Release date
2023-09-07
Hebei Mojin Biotechnology Co., Ltd
Product
Fluorescein isothiocyanate 27072-45-3
Price
US $0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
50000KG/month
Release date
2023-07-28

27072-45-3, Fluorescein isothiocyanate Related Search:

Sodium thiocyanate Ammonium thiocyanate Phenyl isothiocyanate Potassium thiocyanate Erythromycin thiocyanate Fluorescein Fluorescein isothiocyanate isomer I 3-S-Isothiuronium propyl sulfonate BENZYL ISOTHIOCYANATE Isocitric acid trisodium salt Isochlorogenic acid C Guanidine thiocyanate RHODAMINE B ISOTHIOCYANATE FLUORESCEIN ISOTHIOCYANATE-DEXTRAN 2-CHLOROETHYL ISOTHIOCYANATE Ethoxycarbonyl Isothiocyanate Isopropyl isothiocyanate ACETYL ISOTHIOCYANATE

  • FluoresceinIsothiocyanate,Isomers1&11
  • Spiroisobenzofuran-1(3H),9-9Hxanthen-3-one, 3,6-dihydroxy-5(or 6)-isothiocyanato-
  • Fluorescein 5(6)-isothiocyanate,FITC, Fluorescein isothiocyanate
  • Fluorescein5(6)-isot
  • 5(6)-FITC
  • Fluorescein 5(6)-isothiocyanat, mixture of isomers >90%
  • Fluorescein Isothiocyanate (Mixture of 5- and 6- isoMers)
  • Isothiocyanatofluorescein
  • FITC, Fluorescein isothiocyanate
  • Fuorescein isothiocyanate
  • 5-lsothiocyanato fluorescein
  • 5(6)-Fluorescein isothiocyanate
  • Fluorescein isothiocyanate, for fluorescence, Mixture of 5- and 6- isoMers
  • FITC–Celite
  • Fluorescein isothiocyanate isomer I–Celite
  • Flouresceiniso-thiocyanate
  • ocyanato-
  • spiro[isobenzofuran-1(3h),9’-[9h]xanthen]-3-one,3’,6’-dihydroxy-5(or6)-isothi
  • 5(6)-FITC (Fluorescein 5(6)-isothiocyanate)
  • 3',6'-dihydroxy-5(or 6)-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one
  • FLUORESCEIN ISOTHIOCYANATE, FOR FLUORESC ENCE
  • 3',6'-Dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-onecompoundwithisothiocyanatomethane(1:1)
  • Fluorescein 5(6)-isothiocyanate, 95%, for fluorescence, mixture of 5- and 6- isomers
  • SPECTRACOMP(TM) FITC PARTICLES
  • 2-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-5-ISOTHIOCYANATO-BENZOIC ACID
  • 3',6'-Dihydroxy-5(or 6)-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
  • 5-FLUORESCEIN ISOTHIOCYANATE
  • 5-FLUORESCEIN ISOTHIOCYANATE, ISOMER I
  • 5-FLUORESCEIN ISOTHIOCYANATEISOMER
  • FLUORESCEIN-5-ISOTHIOCYANATE ISOMER I
  • FLUORESCEIN 5-ISOTHIOCYANATE, ISOMER 1
  • FLUORESCEIN 5(6)-ISOTHIOCYANATE
  • FLUORESCEIN 5-ISOTHIOCYANATE
  • FLUORESCEIN ISOMER I ISOTHIOCYANATE
  • FLUORESCEIN ISOTHIOCYANATE ISOMER I-CELITE(R)
  • FLUORESCEIN ISOTHIOCYANATE, ISOMER 1
  • FLUORESCEIN ISOTHIOCYANATE
  • FITC ISOMER
  • FITC 'ISOMER I'
  • FITC, ISOMER I
  • FITC-CELITE(R)
  • 3',6'-dihydroxy-6-isothiocyanato-1-spiro[isobenzofuran-3,9'-xanthene]one
  • FluoresceinIsothiocyanate(mixtureof5-and6-isomers)&gt
  • Fluorescein isothiocyanate USP/EP/BP
  • Fluorescein Isothiocyanate (>85%)
  • Fluorescein 5(6)-isothiocyanate, mixture of 2 components, ≥90% (HPLC)
  • 5(6)-FITC (Fluorescein 5(6)-isothiocyanate), Fluorescent amine-reactive label
  • 27072-45-3
  • 3326-94-8
  • 2C21H11NO5S
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