4-Nitrofluorescein

Product Name: 4-Nitrofluorescein
CAS No.: 3326-35-0
Chemical Name: 4-Nitrofluorescein
Synonyms: 4-NITROFLUORESCEIN;4-NITROFLUOROESCEIN;5-Nitrofluorescein (isomer I);5-Nitrofluorescein(isomerI)>3',6'-dihydroxy-6-nitrospiro[2-benzofuran-3,9'-xanthene]-1-one;3',6'-dihydroxy-6-nitro-spiro[2-benzofuran-3,9'-xanthene]-1-one;3',6'-dihydroxy-6-nitro-spiro[isobenzofuran-3,9'-xanthene]-1-one;6,11-dioxo-12-naphtho[2,3-b]indolizinecarboxylic acid ethyl ester;3',6'-Dihydroxy-5-nitro-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one;3',6'-Dihydroxy-5-nitrospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
CBNumber: CB8768159
Molecular Formula: C20H11NO7
Formula Weight: 377.3
MOL File: 3326-35-0.mol

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4-Nitrofluorescein Property

Melting point:: >300°C
Boiling point:: 692.0±55.0 °C(Predicted)
Density: 1.72±0.1 g/cm3(Predicted)
storage temp.: 2-8°C
solubility: almost transparency in NaOH100g/L
form: powder to crystal
pka: 9.29±0.20(Predicted)
color: Light yellow to Brown
Stability:: Light and Moisture Sensitive
InChI: InChI=1S/C20H11NO7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)20(15)14-4-1-10(21(25)26)7-13(14)19(24)28-20/h1-9,22-23H
InChIKey: UACQOMKZFGNRBL-UHFFFAOYSA-N
SMILES: C12(C3=C(C=C(O)C=C3)OC3=C1C=CC(O)=C3)C1=C(C=C([N+]([O-])=O)C=C1)C(=O)O2
EPA Substance Registry System: Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-nitro- (3326-35-0)

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Safety

TSCA: TSCA listed
HS Code: 2932.99.7000

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P280Wear protective gloves/protective clothing/eye protection/face protection.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

TCI Chemical

Product number: N0199
Product name: 5-Nitrofluorescein (isomer I)
Purity: >98.0%(T)
Packaging: 1g
Price: $199
Updated: 2025/07/31

TCI Chemical

Product number: N0199
Product name: 5-Nitrofluorescein (isomer I)
Purity: >98.0%(T)
Packaging: 5g
Price: $695
Updated: 2025/07/31

TRC

Product number: N495860
Product name: Nitrofluorescein,Isomer1
Packaging: 10g
Price: $875
Updated: 2021/12/16

American Custom Chemicals Corporation

Product number: CHM0012147
Product name: 4-NITROFLUORESCEIN
Purity: 95.00%
Packaging: 10G
Price: $2158.7
Updated: 2021/12/16

Chem-Impex

Product number: 38567
Product name: 5-Nitrofluorescein(isomerI),≥98%(Assaybytitration)
Purity: ≥98%(Assaybytitration)
Packaging: 5G
Price: $789.73
Updated: 2021/12/16

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4-Nitrofluorescein Chemical Properties,Usage,Production

Chemical Properties

Orange Cyrstalline Solid

Uses

Fluorescent labeling reagent for proteins. 4-Nitrofluorescein is used in the fluorescent antibody technique for rapid identification of pathogens.

Synthesis

4-Nitrofluorescein was prepared as follows: 8.6 g (18.7 mmol) of ethyl 4-nitrofluorescein triacetate was dissolved in 87 mL of 10% sodium hydroxide solution and 432 mL of methanol and gently heated. The precipitate was filtered and dissolved in 500 mL of water. The solution was acidified with hydrochloric acid and allowed to stand overnight in the dark. The crystals were filtered out and washed with water. 6.9 g (18.2 mmol), 97% red crystals of 4-nitrofluorescein were obtained.C20H11NO7; molecular weight: 377.30; 1HNMR (300 MHz, DMSO-d6)δ 10.20 (brs, 2H, OH), 8.65 (s, 1H, nsi-4H), 8.57 (d, J=8.4. 1H, nsi-6H), 7.57 (d, J=8.3, 1H, nsi-7H), 6.70-6.64 (m, 4H, xa-4, 5, 2, 7H), 6.56 (d, J=8.7, 2H, xa-1, 8H). esims: 376.1 [M-H+] (100).

4-Nitrofluorescein Preparation Products And Raw materials

Raw materials

Preparation Products

Fluorescein isothiocyanate

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4-Nitrofluorescein Suppliers

Americas 1 Canada 1 China 81 Europe 1 Germany 2 India 2 Japan 1 Switzerland 1 United Kingdom 3 United States 6 Global 99

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Shanghai Jian Chao Chemical Technology Co., Ltd.

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View Lastest Price from 4-Nitrofluorescein manufacturers

Shaanxi Dideu Medichem Co. Ltd

Product: 4-Nitrofluorescein 3326-35-0
Price: US $1.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 20T
Release date: 2024-07-11

3326-35-0, 4-NitrofluoresceinRelated Search:

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4-NITROFLUOROESCEIN

5-NITRO-3',6'-DIHYDROXYSPIRO[ISOBENZOFURAN-1(3H),9'-(9H)XANTHEN]-3-ONE

4-NITROFLUORESCEIN

3',6'-Dihydroxy-5-nitrospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one

5-Nitro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one

3',6'-dihydroxy-6-nitro-spiro[2-benzofuran-3,9'-xanthene]-1-one

3',6'-dihydroxy-6-nitrospiro[2-benzofuran-3,9'-xanthene]-1-one

3',6'-dihydroxy-6-nitro-spiro[isobenzofuran-3,9'-xanthene]-1-one

5-Nitrofluorescein (isomer I)

Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one,3',6'-dihydroxy-5-nitro-

3',6'-Dihydroxy-5-nitro-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one

6,11-dioxo-12-naphtho[2,3-b]indolizinecarboxylic acid ethyl ester

5-Nitrofluorescein(isomerI)&gt

3326-35-0

C20H11NO7

Fluorescent Labels & Indicators