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Fluorescein isothiocyanate isomer I

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Fluorescein isothiocyanate isomer I Basic information

Product Name:
Fluorescein isothiocyanate isomer I
Synonyms:
  • FITC
  • FITC-CELITE(R)
  • FITC ISOMER
  • FITC 'ISOMER I'
  • FITC, ISOMER I
  • FLUORESCEIN ISOMER I ISOTHIOCYANATE
  • FLUORESCEIN ISOTHIOCYANATE, ISOMER 1
  • FLUORESCEIN ISOTHIOCYANATE ISOMER I
CAS:
3326-32-7
MF:
C21H11NO5S
MW:
389.38
EINECS:
222-042-0
Product Categories:
  • marker
  • Fluorescent
  • Phenyl isocyanate&Phenyl isothiocyanate
  • Fluorescent Labels and Indicators
  • Amino Group Labeling Reagents for Fluorescence HPLC
  • Xanthene
  • Analytical Chemistry
  • Fluorescence Detection (HPLC Labeling Reagents)
  • HPLC Labeling Reagents
  • Fluorescent Labels & Indicators
Mol File:
3326-32-7.mol
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Fluorescein isothiocyanate isomer I Chemical Properties

Melting point:
>360 °C(lit.)
Boiling point:
708.6±60.0 °C(Predicted)
Density 
1.3423 (rough estimate)
refractive index 
1.5060 (estimate)
storage temp. 
2-8°C
solubility 
Slightly soluble in DMSO, ethanol, methanol DMF and acetone
pka
2.2, 4.4, 6.7(at 25℃)
form 
powder
color 
White
PH Range
Weak green uorescence (6.0) to strong green uorescence (7.2)
Water Solubility 
practically insoluble
λmax
490nm
Sensitive 
Moisture Sensitive
BRN 
1407295
Stability:
Stability Stable, but moisture-sensitive. Incompatible with strong oxidizing agents, strong bases, amines, acids.
Major Application
Magnetic nanowires, immunoassays, analyzing proteins, identifying chromosomes, Salmonella mosomes, diagnosis of cancer, kidney diseases, detecting pathogens, genes, Salmonella cells, toxoplasmosis, enzyme-mediated nucleic acid cleavage, surface molecules on colorectal cancers, treating cancer, chronic lymphocytic leukemia
Biological Applications
Analyzing sugar chain; detecting iodide/iodate or total iodine; detecting/imaging thiols; detecting/quantifying biotin derivatives;16 diagnosing classical Hodgkin lymphoma (CHL) in lymph nodes; cellulose nanocrystals for bioimaging applications; treating neuroinflammation; treating/diagnosing cancer; PEO-PCL-PEO triblock copolymers for topical delivery; polymersomes in biomedical or cosmetic applications;apoptosis assay; as a substrate for measuring heparanase activity, human immunodeficiency virus (HIV-1) proteinase activity, kinase activity, phosphatase activity, β-lactamase activity, transglutaminase (TG2) activity; implantable drug-delivery devicess
InChIKey
MHMNJMPURVTYEJ-UHFFFAOYSA-N
CAS DataBase Reference
3326-32-7(CAS DataBase Reference)
EPA Substance Registry System
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato- (3326-32-7)
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Safety Information

Hazard Codes 
Xn,Xi
Risk Statements 
42/43-36/37/38-22
Safety Statements 
22-45-36/37/39-26-36/37-36
WGK Germany 
3
10
TSCA 
Yes
HS Code 
29329990

MSDS

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Fluorescein isothiocyanate isomer I Usage And Synthesis

Chemical Properties

Orange-yellow cryst.

Uses

Fluorescein -5-isothiocyanate is a kind of fluorescent marker for biochemical applications. Reacts under mild conditions with primary amines and it is also used in modification of actin at lys-61, labelling of FAB and the modification of thiol groups.

Uses

Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens

Definition

ChEBI: The 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

Purification Methods

It is made from the pure 5-amino isomer. Purify it by dissolving it in boiling Me2CO, filtering and adding pet ether (b 60-70o) until it becomes turbid. If an oil separates, then decant it and add more pet ether to the supernatant and cool. Orange-yellow crystals separate, collect and dry them in vacuo. It should give one spot on TLC (silica gel) in EtOAc/pyridine/AcOH (50:1:1) and in Me2NCHO/CHCl3/28% NH4OH (10:5:4). IR (Me2SO): 2110 (NCS) and 1760 (C=O) cm-1. The max 1HNMR spectra in Me2CO-d6 of the 5-and 6-isomers are distinctly different for the protons in the *benzene ring; the UV in phosphate buffer pH 8.0 shows a at ~490nm. [Sinsheimer et al. Anal Biochem 57 2271974, McKinney et al. Anal Biochem 7 74 1964, Beilstein 19 III/IV 4337.]

Fluorescein isothiocyanate isomer ISupplier

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