4-NITRO-3-(OCTANOYLOXY)BENZOIC ACID Chemical Properties

Melting point:

146 - 148°C

storage temp. 

RT

solubility 

Soluble in DMSO (up to 25 mg/ml) or in Ethanol (up to 25 mg/ml).

form 

Off-white solid.

color 

White

Stability:

Stable for 2 years from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 3 months

4-NITRO-3-(OCTANOYLOXY)BENZOIC ACID Usage And Synthesis

Description

NOBA (55894-52-5) is a chromogenic substrate for phospholipases such as PLA2. Read absorbance at 425 nm.

Uses

4-Nitro-3-[(1-oxooctyl)oxy]benzoic acid is used to acrylate ε-amino groups of Lysine (L468895) in order to immobilize phospholipase A2 in snake venom.

References

1) Cho & Kezdy (1991), Chromogenic Substrates and assay of phospholipases A2; Methods Enzymol., 197 75 2) Petrovic et al. (2001), A simple assay for a human serum phospholipase A2 that is associated with high density lipoproteins; J. Lipid Res., 42 1706 3) Bonfim et al. (2008), Structural and functional characterization of myotoxin, Cr-IV 1, a phospholipase A2 D49 from the venom of the snake Calloselasma rhodostoma; Biologicals, 36 168

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