COELENTERAZINE H
COELENTERAZINE H Basic information
- Product Name:
- COELENTERAZINE H
- Synonyms:
-
- Renilla Luciferin
- H-coelenterazine
- 2-Deoxycoelenterazine Luciferin (Renilla)
- Colenterazine H
- 2,8-dibenzyl-6-(4-hydroxyphenyl)iMidazo[1,2-a]pyrazin-3(7H)-one
- CLZN h [Coelenterazine h]
- CLZ-H
- CLZN-H
- CAS:
- 50909-86-9
- MF:
- C26H21N3O2
- MW:
- 407.46
- Product Categories:
-
- Heterocycle
- Imidazo[1,2-a]pyrazin-3(7H)-one
- Mol File:
- 50909-86-9.mol
COELENTERAZINE H Chemical Properties
- Melting point:
- >200℃
- Boiling point:
- 593.5±60.0 °C(Predicted)
- Density
- 1.26±0.1 g/cm3(Predicted)
- storage temp.
- -20°C
- solubility
- Soluble in methanol, ethanol
- pka
- 10.50±0.30(Predicted)
- form
- solid
- color
- Yellow
- λmax
- 437nm
- Stability:
- Stable for 1 year from ate of purchase as supplied. Solutions in DMSO or methanol may be stored at -20°C for up to 1 month.
- Biological Applications
- Calcium indicator; assaying luminescent enzyme; measuring luciferase activity; as a substrate for luciferase; screening HIV-1 protease inhibitors; bioluminescence resonance energy transfer (BRET) detection system
COELENTERAZINE H Usage And Synthesis
Description
Coelenterazine-h (50909-86-9) may be used as a substrate in gene reporter assays, live cell assays, and biochemical assays (ELISA, BRET etc). Renilla luciferase catalyzes coelenterazine oxidation by oxygen to produce light. Coelenterazine-h produces 10-fold higher light output than native coelenterazine. Coelenterazine-h also undergoes autoluminescence, which is enhanced by superoxide anion and peroxynitrite in cells.
Uses
Coelenterazine h, is a derivative of Coelenterazine (C636000), and iis more sensitive to Ca2+ than is the native complex, thus providing a valuable tool for measuring small changes in Ca2+ concentrations.
Definition
ChEBI: Renilla luciferin is a member of phenols and an imidazopyrazine. It has a role as a luciferin. It derives from a hydride of an imidazo[1,2-a]pyrazine.
General Description
Coelenterazine is an imidazopyrazinone derivative. It is a substrate for bioluminescence in many luminous marine organisms.
Biochem/physiol Actions
10 times higher luminescence intensity than native coelenterazine.
References
1) Shimomura?et al. (1989),?Semi-synthetic aequorins with improved sensitivity to Ca2+ ions; Biochem. J.,?261?913 2) Nakajima-Shimada?et al.?(1991),?Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system; Proc. Natl. Acad. Sci. USA,?88?6878 3) Inouye?et al. (1992),?Monitoring gene expression in Chinese hamster ovary cells using secreted apoaequorin; Anal. Biochem.,?201?114
COELENTERAZINE HSupplier
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COELENTERAZINE H(50909-86-9)Related Product Information
- Coelenterazine
- COELENTERAZINE-HCP, SYNTHETIC,COELENTERAZINE HCP
- CLA
- COELENTERAZINE CP
- DI-DEHYDRO COELENTERAZINE,COELENTERAZINE 400 A
- COELENTERAZINE F,COELENTERAZINE-F, SYNTHETIC
- COELENTERAZINE H
- ALPHA-LUCIFERIN
- COELENTERAZINE FCP
- Coelenterazine ch
- Coelenterazine fch
- COELENTERAZINE E
- COELENTERAZINE I
- Coelenterazine-Ws
- Coelenterazine ip:Ca2+ Dye, bioluminescence
- Coelenterazine cp (Ca2+ Dye,):Ca2+ Dye, bioluminescence
- Coelenterazine h:Ca2+ Dye, bioluminescence
- Coelenterazine O