Basic information Safety Supplier Related

COELENTERAZINE H

Basic information Safety Supplier Related

COELENTERAZINE H Basic information

Product Name:
COELENTERAZINE H
Synonyms:
  • Renilla Luciferin
  • H-coelenterazine
  • 2-Deoxycoelenterazine Luciferin (Renilla)
  • Colenterazine H
  • 2,8-dibenzyl-6-(4-hydroxyphenyl)iMidazo[1,2-a]pyrazin-3(7H)-one
  • CLZN h [Coelenterazine h]
  • CLZ-H
  • CLZN-H
CAS:
50909-86-9
MF:
C26H21N3O2
MW:
407.46
Product Categories:
  • Heterocycle
  • Imidazo[1,2-a]pyrazin-3(7H)-one
Mol File:
50909-86-9.mol
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COELENTERAZINE H Chemical Properties

Melting point:
>200℃
Boiling point:
593.5±60.0 °C(Predicted)
Density 
1.26±0.1 g/cm3(Predicted)
storage temp. 
-20°C
solubility 
Soluble in methanol, ethanol
pka
10.50±0.30(Predicted)
form 
solid
color 
Yellow
λmax
437nm
Stability:
Stable for 1 year from ate of purchase as supplied. Solutions in DMSO or methanol may be stored at -20°C for up to 1 month.
Biological Applications
Calcium indicator; assaying luminescent enzyme; measuring luciferase activity; as a substrate for luciferase; screening HIV-1 protease inhibitors; bioluminescence resonance energy transfer (BRET) detection system
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Safety Information

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-36
WGK Germany 
3
HS Code 
2933998090
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COELENTERAZINE H Usage And Synthesis

Description

Coelenterazine-h (50909-86-9) may be used as a substrate in gene reporter assays, live cell assays, and biochemical assays (ELISA, BRET etc). Renilla luciferase catalyzes coelenterazine oxidation by oxygen to produce light. Coelenterazine-h produces 10-fold higher light output than native coelenterazine. Coelenterazine-h also undergoes autoluminescence, which is enhanced by superoxide anion and peroxynitrite in cells.

Uses

Coelenterazine h, is a derivative of Coelenterazine (C636000), and iis more sensitive to Ca2+ than is the native complex, thus providing a valuable tool for measuring small changes in Ca2+ concentrations.

Definition

ChEBI: Renilla luciferin is a member of phenols and an imidazopyrazine. It has a role as a luciferin. It derives from a hydride of an imidazo[1,2-a]pyrazine.

General Description

Coelenterazine is an imidazopyrazinone derivative. It is a substrate for bioluminescence in many luminous marine organisms.

Biochem/physiol Actions

10 times higher luminescence intensity than native coelenterazine.

References

1) Shimomura?et al. (1989),?Semi-synthetic aequorins with improved sensitivity to Ca2+ ions; Biochem. J.,?261?913 2) Nakajima-Shimada?et al.?(1991),?Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system; Proc. Natl. Acad. Sci. USA,?88?6878 3) Inouye?et al. (1992),?Monitoring gene expression in Chinese hamster ovary cells using secreted apoaequorin; Anal. Biochem.,?201?114

COELENTERAZINE HSupplier

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