PYRONIN Y Chemical Properties

Melting point:

250-260 °C(lit.)

storage temp. 

room temp

solubility 

Soluble in water; sparingly soluble in ethanol, ethylene glycol, methyl cellosolve

Colour Index 

45005

form 

Powder/Solid

color 

Pink

Water Solubility 

Soluble in water. Also soluble in methyl cellosolve (9 mg/mL) and ethanol (3 mg/mL).

λmax

548 nm

Merck 

13,8097

BRN 

3795789

Stability:

Stable. Incompatible with strong oxidizing agents.

Biological Applications

Antimalarial agent; antiviral agent; nucleic acid sequencing; treating protozoan infections; herbicides

InChIKey

INCIMLINXXICKS-UHFFFAOYSA-M

CAS DataBase Reference

92-32-0

EPA Substance Registry System

Pyronine Y (92-32-0)

Safety Information

Hazard Codes 

Xn,Xi

Risk Statements 

68-36/37/38

Safety Statements 

22-24/25-36-26

WGK Germany 

3

RTECS 

BQ1450000

TSCA 

Yes

HS Code 

32041300

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS

PYRONIN Y Usage And Synthesis

Chemical Properties

green crystalline solid

Uses

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

Uses

Pyronin Y has been used:

• in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
• as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
• staining of CD34⁺ cells

Uses

Bacterial and biological stain.

Definition

ChEBI: An organic chloride salt having 6-(dimethylamino)-N,N-dimethyl-3H-xanthen-3-iminium as the cation. Used with methyl green to selectively demonstrate RNA (red) in contrast to DNA (green) with the Unna Pappenheim method.

General Description

Pyronin Y (C.I. 45005) is an aminoxanthene dye that is present as a weakly lipophilic cation in neutral aqueous solutions. It is also known as pyronine G.

Biochem/physiol Actions

Pyronin Y is important in histochemistry and is combined with methyl green for staining of RNA. The mixture results in bright red/green staining of nucleic acids.

storage

Room temperature

Purification Methods

Commercial material may contain a large quantity of zinc. Purify it by dissolving 1g in 50mL of hot water containing 5g NaEDTA. Cool to 0o, filter, evaporate to dryness and the residue is extracted with EtOH. The solution is evaporated to 5-10mL, filtered, and the dye is precipitated by addition of excess of dry diethyl ether. It is centrifuged, and the crystals are washed with dry ether. The procedure is repeated, then the product is dissolved in CHCl3, filtered and evaporated. The dye is stored in a vacuum. [Beilstein 18 H 596, 18 III/IV 7361, 18/10 V 181.]

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