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Fuchsin Acid

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Fuchsin Acid Basic information

Product Name:
Fuchsin Acid
Synonyms:
  • FUCHSIN TRISULFONATE
  • FUCHSIN VIOLET
  • FUCHSIN ACID
  • FUCHSINE ACID
  • FUCHSIN ACIDIC DISODIUM SALT
  • CI NO 42685
  • CI 42685
  • MAGENTA ACID
CAS:
3244-88-0
MF:
C20H17N3Na2O9S3
MW:
585.54
EINECS:
221-816-5
Product Categories:
  • marker
  • Triphenylmethane
Mol File:
3244-88-0.mol
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Fuchsin Acid Chemical Properties

Melting point:
>250°C
storage temp. 
Store at RT.
solubility 
H2O: soluble1mg/mL
form 
Solid
Colour Index 
42685
color 
Very dark green
PH
3-4 (10g/l, H2O, 20℃)
PH Range
5(pink)-8(yellow)
Water Solubility 
14.3 g/100 mL
λmax
546nm
Merck 
14,107
BRN 
4111656
Stability:
Stable. Incompatible with strong oxidizing agents.
Biological Applications
Detecting enzyme activity,proteins,tumor cells
Major Application
Nanocomposite, recording materials, sol-gel matrix, decoder system, color filter, inks, paints, markers, highlighters, corrosion inhibitors, explosives, packaging system, textiles, hair dyes, make-up, cosmetics, food storage, dental bleaching, detecting turmor cells, determine bacterial growth, cancer chemopreventive activity
EPA Substance Registry System
C.I. Acid Violet 19 (3244-88-0)
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Safety Information

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26-36-24/25
WGK Germany 
3
RTECS 
DD4737000
TSCA 
Yes
HS Code 
32041200

MSDS

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Fuchsin Acid Usage And Synthesis

Description

Fuchsin acid (Acid magenta) is a red, acid dye of the triphenylmethane series, consisting of the sodium salts of the sulphonic acids of basic fuchsin or of the individual dyes (pararosaniline, rosaniline and new fuchsin), of which the latter is a mixture. The composition of fuchsin acid, like fuchsin basic, can vary with the country of origin and from one manufacturer to another.
Industrial uses of the dye include the colouring of crepe tissues, soap and photographic films and the dyeing of leather, which it penetrates easily.
A 1 % solution of Microme No. 5 in deionized or distilled water (previously adjusted to pH 7-0 with alkali) has a pH of 5-0.
Fuchsin acid is decolorized in solution by the addition of NaOH and in this form it is used as an indicator (known as Andrade's indicator) : this finds application in bacteriology for detecting acid-forming organisms.
Very dilute aqueous solutions of the dye in London tap water (pH about 8-1) fade within two or three seconds. In deionized water they retain their colour considerably longer, however.

Chemical Properties

dark green crystalline powder

Uses

As pH indicator; biological stain.

Biotechnological Applications

Fuchsin acid has very many applications in biological microtechnique. Gray (1954) describes around two hundred of these.
It was discovered some years ago (MacConaill and Gurr, 1960; Gurr, 1962) that fuchsin acid forms complexes with light green SF (yellowish), conjugation taking place apparently between the amino groups of the first and the sulphonic groups of the second dye. These interactions were first discovered by Professor M .Conaill of University College, Cork, in 1958. About two years later one of these complexes (a blue dye which was given the name of Trifalgic acid; synonyms: MG blue, Falg blue) was synthesized in the solid state by the writer and found useful application in clinical pathology as a differential stain for proteins in electrophoresis (Bodman, 1960).
MacConailPs discovery was made during the course of an investigation into the chemical and physical nature of certain substances present in mammalian tissues and cells. Histological sections were stained first with the red anionic dye, fuchsin acid, then counterstained with the anionic dye, light green SF (yellowish). It was naturally expected that these dyes would impart a dichrome picture, in red and green. However, when he came to examine the sections under the microscope the picture seen was not in red and green but scarlet, various shades of violet and pure blue. The blue coloration excited his interest in particular. Although he considered at first that its presence was probably due to physical causes, subsequent tests carried out by him appeared to suggest that chemical interactions between the red and the green dye had taken place with the formation of at least three new dyes, one of which was blue. Cumulative and circumstantial evidence resulting from histological, physical and chemical experiments, since carried out over a period of years, leave no room for doubt that fuchsin acid and similar anionic dyes which have basic colligators (e.g. primary amino groups), can and do, under appropriate conditions, interact both in vitro and in tissues with other anionic dyes to form compounds which are polyanionic dyes.

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