sulfo-Cyanine3.5 NHS ester CAS#: 2231670-91-8

sulfo-Cyanine3.5 NHS ester Basic information

Product Name:

sulfo-Cyanine3.5 NHS ester

Synonyms:

sulfo-Cyanine3.5 NHS ester
Sulfo cy3.5 NHS ester
sulfo Cy3.5(Me)SE.TEA
3-(6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-1,1-dimethyl-2-(3-(1,1,3-trimethyl-6,8-disulfonato-1,3-dihydro-2H-benzo[e]indol-2-ylidene)prop-1-en-1-yl)-1H-benzo[e]indol-3-ium-6,8-disulfonate
Sulfo-Cy3.5-M SE (Orange Red)

CAS:

2231670-91-8

MF:

C42H40N3O16S4(-3)

MW:

971.03

Mol File:

2231670-91-8.mol

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sulfo-Cyanine3.5 NHS ester Chemical Properties

solubility: Easily soluble in methanol, DMF and other organic solvents, very soluble in water
Appearance: Dark purple solid
ex/em: 584/604 nm (PBS buffer)
ε(extinction coefficient): 139000 L⋅mol−1⋅cm−1
Φ(quantum yield): 0.11

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sulfo-Cyanine3.5 NHS ester Usage And Synthesis

Description

Cyanine3.5 is a fluorescent dye with absorption and emission intermediate between Cyanine3 and Cyanine5. The sulfonated version contains four sulfo groups, which ensure high solubility and hydrophilicity of the fluorophore and labeled conjugates.

The NHS ester reactive group allows labeling of primary and secondary amine groups.

Chemical Properties

Appearance: Dark purple solid
ex/em: 584/604 nm (PBS buffer)
Extinction coefficient (ε): 139000 L??mol??1??cm??1
Quantum yield (Φ): 0.11

Abs/Em Maxima

576/603 nm

Fluoresene quantum yield

0.11

Extinction Coefficient

139000

CF260

0.16

CF280

0.17

sulfo-Cyanine3.5 NHS esterSupplier

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