diSulfo-Cy5 azide Chemical Properties

solubility 

Soluble in Water, DMSO, DMF, DCM

Appearance

Red powder

ex/em

550/566 nm (PBS buffer)

ε(extinction coefficient)

162000 L⋅mol−1⋅cm−1

Φ(quantum yield)

0.1

diSulfo-Cy5 azide Usage And Synthesis

Description

diSulfo-Cy3 azide is a water-soluble dye containing an azide group, which enables Click Chemistry. Absorbance and emission of the dye are identical to Cy3 fluorophore. The reagent is designed for the labeling of sensitive molecules such as proteins, and even intact biological objects in water phase.

Chemical Properties

Appearance: Red powder
ex/em: 550/566 nm (PBS buffer)
Extinction coefficient (ε): 162000 L??mol??1??cm??1
Quantum yield (Φ): 0.1

Uses

Sulfo-cyanine3 azide sodium a water-soluble azide cyanine dye and fluorescent probe (Ex/Em=548/563 nm). Sulfo-cyanine3 azide sodium can be involved in synthesis of fused tricyclic heterocycles, produces immunostimulatory agents[1][2]. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.

References

[1] Hsieh PY, et al. Harnessing Fluorescent Moenomycin A Antibiotics for Bacterial Cell Wall Imaging Studies. Chembiochem. 2021 Dec 10;22(24):3462-3468. DOI:10.1002/cbic.202100433
[2] David, Sunil A, et al. Preparation of fused tricyclic heterocycles as therapeutic compounds and methods of use thereof: World Intellectual Property Organization, WO2020009946[P]. 2020-01-09.

Abs/Em Maxima

548/563 nm

Fluoresene quantum yield

0.1

Extinction Coefficient

162000

CF260

0.03

CF280

0.06

diSulfo-Cy5 azide(2055138-89-9)Related Product Information

• 3H-IndoliuM, 2-[5-[1-[6-[(3-azidopropyl)aMino]-6-oxohexyl]-1,3-dihydro-3,3-diMethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1,3,3-triMethyl-5-sulfo-, inner salt
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• diSulfo-Cy3 alkyne
• Trisulfo-Cy5.5-Alkyne
• Cy5.5 Acid (tri SO3)
• disulfo-ICG-DBCO
• disulfo-ICG-azide
• triSulfo-Cy5.5 DBCO
• trisulfo-Cyanine3.5-carboxylic acid
• Cyanine 7 tetrazine