N-methylisatoic anhydride Chemical Properties

Melting point:

165 °C (dec.)(lit.)

Boiling point:

309.06°C (rough estimate)

Density 

1.3312 (rough estimate)

vapor density 

6.1 (vs air)

refractive index 

1.5200 (estimate)

storage temp. 

Inert atmosphere,Room Temperature

solubility 

Soluble in DMSO

form 

Solid

pka

-2.24±0.20(Predicted)

color 

White to yellow

Sensitive 

Moisture Sensitive

InChI

InChI=1S/C9H7NO3/c1-10-7-5-3-2-4-6(7)8(11)13-9(10)12/h2-5H,1H3

InChIKey

KJMRWDHBVCNLTQ-UHFFFAOYSA-N

SMILES

N1(C)C2=CC=CC=C2C(=O)OC1=O

CAS DataBase Reference

10328-92-4(CAS DataBase Reference)

EPA Substance Registry System

2H-3,1-Benzoxazine-2,4(1H)-dione, 1-methyl- (10328-92-4)

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-37/39

WGK Germany 

3

RTECS 

DM3140000

TSCA 

Yes

HS Code 

2934.99.4400

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS
• Language:English Provider:ALFA

N-methylisatoic anhydride Usage And Synthesis

Chemical Properties

brownish chunks

Uses

N-Methylisatoic anhydride is a 2'-OH selective acylation agent of RNAs, and is widely used for resolving secondary RNA structures using the SHAPE (Selective 2'-Hydroxyl Acylation Analyzed by Primer Extension) technology.

Definition

ChEBI: N-methylisatoic anhydride is a 3,1-benzoxazin-1,4-dione having an N-methyl substituent.

Application

N-Methylisatoic anhydride was first condensed with ethanolamine to give N-(2-hydroxyethyl)-2-methylaminobenzamide. Cyclization of the latter with ethyl pyruvate gave quinazoline derivatives which, upon hydrolysis and dehydrative cyclization with l-methyl-2-chloropyridinium iodide, afforded the l,4-oxazines[3,4-]quinazoline.

Description

Melanoma Inhibitory Activity Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain consisting of 108 amino having a total molecular mass of 12237 Dalton.
The MIA is purified by proprietary chromatographic techniques.

Source

Escherichia Coli

Background

The Melanoma Inhibitory protein (MIA) was identified as an inhibitor of in vitro growth of malignant melanoma cells. The protein contains a SH3 domain.
MIA acts as a potent tumor cell growth inhibitor for malignant melanoma cells and some other neuroectodermal tumors, including gliomas, in an autocrine fashion. In a study of human melanoma cell lines with different metastatic capacity MIA mRNA expression appeared to be inversely correlated with pigmentation. MIA has been shown to represent a very sensitive and specific serum marker for systemic malignant melanoma that might be useful for staging of primary melanomas, detection of progression from localized to metastatic disease during follow-up, and monitoring therapy of advanced melanomas.

N-methylisatoic anhydride(10328-92-4)Related Product Information

• 5-Chloro-N-methylisatoic anhydride
• N-methylisatoic anhydride
• Ethyl 4-dimethylaminobenzoate
• Isatoic Anhydride
• 1-ISOPROPYL-1H-BENZO[D][1,3]OXAZINE-2,4-DIONE
• N-BENZYLISATOIC ANHYDRIDE
• 5-CHLORO-1-METHYL-1H-BENZO[D][1,3]OXAZINE-2,4-DIONE
• 3-METHOXY-N-METHYLISATOIC ANHYDRIDE
• N-ALLYLISATOIC ANHYDRIDE
• 6-Methylisatoic anhydride
• 5-METHYLISATOIC ANHYDRIDE
• 7-FLUORO-2-METHYLISATOIC ANHYDRIDE
• AMILORIDE, 5-(N-METHYL-N-ISOBUTYL) (MIA) >97% EXTREMELY POTE,MIA
• 6-CHLORO-N-METHYLISATOIC ANHYDRIDE
• N-METHYLISATOIC ANHYDRIDE POLYSTYRENE (100-200 MESH, 3 MMOL/G)
• HUMAN MIA
• KG POA 5-METHYLISATOIC ANHYDRIDE
• MIA protein