Sulfo-Cyanine5.5 azide Chemical Properties

solubility 

Soluble in methanol, DMF and other organic solvents, easily soluble in water

Appearance

Dark blue solid

ex/em

678/706 nm (PBS buffer)

ε(extinction coefficient)

211000 L⋅mol−1⋅cm−1

Φ(quantum yield)

0.21

Sulfo-Cyanine5.5 azide Usage And Synthesis

Description

sulfo-Cyanine5.5 is a water-soluble, hydrophilic fluorophore with emission in the far-red area of the spectrum, an analog of Cy5.5^®. Like other cyanine dyes, sulfo-Cyanine5.5 has an outstanding molar extinction coefficient, giving rise to its bright fluorescence. The molecule contains four sulfo groups that provide hydrophilicity and negative charge to the fluorophore — this minimizes non-specific binding.

The azide group of Sulfo-Cyanine5.5 azide can be conjugated with terminal alkynes in the presence of copper(I) catalyst or with cycloalkynes in copper-free strain-promoted reaction.

The reagent possesses high hydrophilicity and is recommended for labeling biomolecules in an aqueous environment.

Chemical Properties

Appearance: Dark blue solid
ex/em: 678/706 nm (PBS buffer)
Extinction coefficient (ε): 211000 L??mol??1??cm??1
Quantum yield (Φ): 0.21

Uses

Sulfo-Cyanine5.5 azide tripotassium, a near-infrared (NIR) fluorescent dye, is an azide-functionalized Sulfo-Cyanine5.5 (Ex=678 nm, Em=694 nm). Sulfo-Cyanine5.5 azide tripotassium can react with alkynes for Click Chemistry labeling[1]. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.

References

[1] Katrin Spinnler, et al. An Alkyne-functionalized Arginine for Solid-Phase Synthesis Enabling "Bioorthogonal" Peptide Conjugation. ACS Med Chem Lett. 2019 Sep 30;11(3):334-339. DOI:10.1021/acsmedchemlett.9b00388

Abs/Em Maxima

673/691 nm

Fluoresene quantum yield

0.21

Extinction Coefficient

211000

CF260

0.09

CF280

0.11

Sulfo-Cyanine5.5 azide(2382994-65-0)Related Product Information

• Sulfo-Cyanine5 carboxylic acid
• Cy7
• Sulfo-Cy7.5 maleimide
• Sulfo-Cy7.5 carboxylic acid
• Sulfo-Cyanine5.5 carboxylic acid
• Anti-STAT3 Antibody
• Sulfo-CY7-PEG2K-NHS
• Sulfo-Cy7 azide
• Sulfo-CY7-Thiol
• Sulfo-Cy7.5 NHS este
• Cyanine5.5 amine
• Cyanine5.5 maleimide
• diSulfo-Cy5 alkyne
• 1H-Benz[e]indolium, 2-[5-[3-[6-[(3-azidopropyl)amino]-6-oxohexyl]-1,3-dihydro-1,1-dimethyl-2H-benz[e]indol-2-ylidene]-1,3-pentadien-1-yl]-1,1,3-trimethyl-, chloride (1:1)
• Sulfo-Cyanine5.5 maleimide
• Sulfo-Cy5.5 NHS ester
• Sulfo-Cy5 bis-NHS ester
• Sulfo-Cyanine5.5 alkyne