H-8 DIHYDROCHLORIDE Chemical Properties

Melting point:

221-222℃ (methanol water )

storage temp. 

2-8°C

solubility 

ethanol: 25 mg/mL, clear, colorless

form 

Lyophilized

color 

White to Off-White

Water Solubility 

water: 10mg/mL

InChI

InChI=1S/C12H15N3O2S.2ClH/c1-13-7-8-15-18(16,17)12-4-2-3-10-9-14-6-5-11(10)12;;/h2-6,9,13,15H,7-8H2,1H3;2*1H

InChIKey

RJJLZYZEVNCZIW-UHFFFAOYSA-N

SMILES

C12=CC=NC=C1C=CC=C2S(=O)(=O)NCCNC.Cl.Cl

CAS DataBase Reference

113276-94-1(CAS DataBase Reference)

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

HS Code 

29334990

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS

H-8 DIHYDROCHLORIDE Usage And Synthesis

Description

The H series isoquinolinesulfonamide protein kinase (PK) inhibitors are widely used to block signaling pathways to elucidate mechanisms of cellular regulation and signal transduction. H-8, an isoquinolinesulfonamide protein kinase (PK) inhibitor, is a potent inhibitor of PKA and PKG and shows moderate inhibition for PKC and MLCK with K_i values of 1.2, 0.48, 15, and 68 μM, respectively. H-8 can disrupt transcriptional elongation by inhibiting cyclin C/Cdk8 and cyclin H/Cdk7/p36 CTD kinase activity with IC₅₀ values of 47 and 6.2 μM, respectively.

Chemical Properties

white to off-white crystalline solid

Uses

Active inhibitor of cyclic-nucleotide-dependent protein kinases.

Biochem/physiol Actions

cAMP and cGMP-dependent protein kinase inhibitor.

References

[1] R A ENGH. Crystal structures of catalytic subunit of cAMP-dependent protein kinase in complex with isoquinolinesulfonyl protein kinase inhibitors H7, H8, and H89. Structural implications for selectivity.[J]. The Journal of Biological Chemistry, 1996, 271 42: 26157-26164. DOI: 10.1074/jbc.271.42.26157
[2] HIROYOSHI HIDAKA. Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide-dependent protein kinase and protein kinase C[J]. Biochemistry Biochemistry, 1984, 23 21: 5036-5041. DOI: 10.1021/bi00316a032
[3] M HAGIWARA  H H  M Inagaki. Specific binding of a novel compound, N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide (H-8) to the active site of cAMP-dependent protein kinase.[J]. Molecular Pharmacology, 1987, 31 5: 523-528.
[4] P RICKERT  E L  J L Corden. Cyclin C/CDK8 and cyclin H/CDK7/p36 are biochemically distinct CTD kinases.[J]. Oncogene, 1999, 18 4: 1093-1102. DOI: 10.1038/sj.onc.1202399

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