diSulfo-Cyanine3 hydrazide Chemical Properties

solubility 

soluble in water, DMF, DMSO

Appearance

dark red solid

diSulfo-Cyanine3 hydrazide Usage And Synthesis

Description

sulfo-Cyanine3 is a sulfated derivative of Cyanine3 dye, well soluble in water because of two negatively charged sulfo groups in its structure. By its spectral characteristics, this yellow-orange fluorescent dye is an analog of Cy™3.

Hydrazides efficiently react with aldehydes and ketones resulting in hydrazones, so this compound can be used for conjugation with carbonyl derivatives of biomolecules.

The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with sulfo-Cyanine3 hydrazide). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can be also conjugated with sulfo-Cyanine3 hydrazide in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.

Uses

Sulfo-Cy3 hydrazide is a Cyanine 3 (Cy3) (HY-D0822) dye derivative with hydrazine functionality. The sulfonate ion increases the water solubility of the compound, making it suitable for use in aqueous solutions. Cy3 is a fluorescent dye with a fluorescence spectrum typically in the green to orange wavelength range. The hydrazide group of Sulfo-Cy3 hydrazide can form hydrazinone coupling with molecules containing aldehydes or ketones to form covalent bonds. Therefore, Cy3 azide plus can bind to biomolecules such as proteins and antibodies to track their location and dynamic changes in biological samples.

Abs/Em Maxima

548/563 nm

Fluoresene quantum yield

0.1

Extinction Coefficient

162000

CF260

0.03

CF280

0.06

diSulfo-Cyanine3 hydrazide(2144762-62-7)Related Product Information

• Cy3
• Sulfo Cyanine3 NHS ester
• diSulfo-Cy3 alkyne
• Sulfo Cy3 Carboxylic acids(methyl)
• diSulfo-Cy3 DBCO(Methyl)
• diSulfo-Cy3 amine
• Gadoteridol
• diSulfo-Cy5 maleimide
• ICG-Alkyne
• diSulfo-Cy3 maleimide
• diSulfo-Cy5 Hydrazide
• diSulfo-Cyanine3 Me-tetrazine
• triSulfo-Cy5.5 Me-Tetrazine
• Sulfo-Cyanine3 maleimide
• diSulfo-Cyanine5 Me-tetrazine
• Sulfo-Cyanine3 amine
• Sulfo-Cyanine3 DBCO