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D-Luciferin potassium salt

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D-Luciferin potassium salt Basic information

Product Name:
D-Luciferin potassium salt
Synonyms:
  • D-(-)-2-[6'-HYDROXY-2'-BENZOTHIAZOLYL]DELTA-THIAZOLINE-4-CARBOXYLIC ACID POTASSIUM SALT
  • D-LUCIFERIN FIREFLY, POTASSIUM SALT
  • D-LUCIFERIN, MONOPOTASSIUM SALT
  • D-LUCIFERIN POTASSIUM SALT
  • BEETLE LUCIFERIN, POTASSIUM SALT
  • 4,5-DIHYDRO-2-(6-HYDROXY-2-BENZOTHIAZOLYL)-4-THIAZOLECARBOXYLIC ACID POTASSIUM SALT
  • D-LUCIFERIN K SALT
  • D -LUCIFERIN SYNTHETIC POTASSIUM SALT
CAS:
115144-35-9
MF:
C11H9KN2O3S2
MW:
320.42
EINECS:
1592732-453-0
Mol File:
115144-35-9.mol
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D-Luciferin potassium salt Chemical Properties

storage temp. 
Inert atmosphere,Store in freezer, under -20°C
solubility 
DMF:16.7(Max Conc. mg/mL);52.45(Max Conc. mM)
DMSO:7.5(Max Conc. mg/mL);23.55(Max Conc. mM)
Ethanol:0.25(Max Conc. mg/mL);0.79(Max Conc. mM)
PBS (pH 7.2):10.0(Max Conc. mg/mL);0.31(Max Conc. mM)
Water:32.0(Max Conc. mg/mL);100.5(Max Conc. mM)
form 
A crystalline solid
color 
Light yellow
BRN 
5416262
InChI
InChI=1/C11H8N2O3S2.K.H/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16;;/h1-3,7,14H,4H2,(H,15,16);;/t7-;;/s3
InChIKey
UMBKGTQQGYPQBE-OGFXRTJISA-M
SMILES
C([C@H]1CSC(C2=NC3C=CC(O)=CC=3S2)=N1)(=O)O.[KH] |&1:1,r|
CAS DataBase Reference
115144-35-9(CAS DataBase Reference)
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Safety Information

WGK Germany 
3
HS Code 
29342000
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D-Luciferin potassium salt Usage And Synthesis

Description

D-Luciferin potassium salt(115144-35-9) is a water soluble form of D-Luciferin. D-Luciferin is a common bioluminescent substrate used for imaging of firefly luciferase expression with peak chemiluminescent emission at 560 nm. The ATP dependant luciferin oxidation is catalyzed by the Luciferase enzyme encoded by lucgene.

Chemical Properties

Yellow powder

Uses

D-Luciferin Potassium Salt is a class of light-emitting heterocyclic compounds found in organisms that cause bioluminescence, such as firefly. This compound creates light through the process of oxidative decarboxylation by luciferase while ATP is present. In cell culture, D-Luciferin Potassium Salt is used to achieve highly sensitive bioluminescence detection. Recently, Luciferin was used to measure the uptake of mRNA in preparation of the vaccine against COVID-19.

Application

D-Luciferin may be used as a substrate in luciferase-based (EC 1.13.12.7) bioluminescence imaging and cell-based high-throughput screening applications. ATP can be measured with a reagent made up of D-luciferin and luciferase from firefly. D-Luciferin may be used and studied as a partial agonist for G protein-coupled receptor-35 (GPR35). Used for imaging the expression of the LUC marker gene and luciferase-fusion conjugates in living cells, tissues and organisms. Extensively utilized in reporter gene assays, immunoassays and hygiene monitoring.

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